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Cited by 113Angiogenesis and VEGF in CancerGlioma Diagnosis and TreatmentCancer, Hypoxia, and Metabolism

The DOI matches its Crossref record; 36 of the 37 references it cites were found in a registry or an index.

Differential downregulation of vascular endothelial growth factor by dexamethasone in normoxic and hypoxic rat glioma cells

Machein, Kullmer, Rönicke, Machein, Krieg, Damert, Breier, Risau, Plate·1999·Neuropathology and Applied Neurobiology
DOI 10.1046/j.1365-2990.1999.00166.x
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Abstract

Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is a mitogen and chemotactic factor for endothelial cells in vitro and an angiogenesis and vascular permeability factor in vivo. Due to its properties, VEGF is a candidate for both angiogenesis and vascular permeability/oedema induction which typically occur in glioblastomas. In this study we test the hypothesis that the antioedema effect of dexamethasone is mediated by downregulation of VEGF or VEGF receptor expression. VEGF mRNA and protein levels of two rat glioma cells lines, C6 and GS‐9L, were determined after incubation with dexamethasone under normoxic and hypoxic conditions. In normoxic C6 and GS9L cells, we observed 50–60% downregulation of VEGF mRNA by dexamethasone (P=0.015 and P=0.01, respectively). This effect was dependent on glucocorticoid‐receptor (GR) function. The inhibitory effect of dexamethasone on VEGF gene expression by tumour cells was markedly reduced by hypoxia which suggests that the upregulation of VEGF driven by hypoxia overcomes the effect of the dexamethasone. Dexamethasone did not alter VEGFR‐2 mRNA levels in human umbilical endothelial cells. In a subcutaneous glioma tumour model, we observed only a 15% decrease in VEGF mRNA expression in dexamethasone treated animals (n=12) compared with controls animals (P=0.24). We conclude that dexamethasone may decrease brain tumour‐associated oedema by reduction of VEGF expression in tumour cells. However, the highly reduced activity on hypoxic tumour cells suggests that dexamethasone efficacy may be limited by hypoxia in rapidly growing tumours.

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Crossref · 10.1046/j.1365-2990.1999.00166.x

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Research works printed but not linked: 4

  1. Criscuolo GR, 1996, The genesis of peritumoural vasogenic brain oedemaand tumour cysts: a hypothetical role for tumour‐derived vascular permeability factor., Yale J Biol Med, 66, 277
  2. Galicich JH, 1961, Use of dexamethasone in treatment of cerebral oedema associated with brain tumours., J Lancet (Minneapolis), 81, 46
  3. Jakeman LB, 1993, Developmental expression of binding sites and messenger ribonucleic acid for vascular endothelial growth factor suggests a role for this protein in vasculogenesis and angiogenesis Endocrinol, 133, 848
  4. Kremer C, 1997, Upregulation of flk‐1/VEGF receptor‐2 by its ligand in a cerebral slice culture system., Cancer Res, 57, 3852

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Metadata

Authors
Machein, Kullmer, Rönicke, Machein, Krieg, Damert, Breier, Risau, & Plate
Corresponding author
Plate
Venue
Neuropathology and Applied Neurobiology
Publisher
Wiley
Published
1999-04
Volume
25
Issue
2
Pages
104-112
ISSN
  • 0305-1846 (ISSN-L)
  • 1365-2990
DOI
10.1046/j.1365-2990.1999.00166.x
OpenAlex
W2133286797
Cited by
113
References
37
Citations by year
2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 6 · 2019: 5 · 2017: 2 · 2016: 5 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 4
FWCI
1.38
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1009 d)
Locations
  • Neuropathology and Applied Neurobiology (publishedVersion)
  • PubMed (publishedVersion)
  • FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Health Sciences › Medicine › Oncology › Glioma Diagnosis and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Keywords
vascular endothelial growth factor, dexamethasone, glioma, hypoxia, glucocorticoid receptor, VEGF receptor 2, angiogenesis, VEGF expression, C6 cells
MeSH terms
Animals, Cell Line, Dexamethasone, Endothelium, Vascular, Estradiol, Glioma, Glucocorticoids, Humans, Lymphokines, Progesterone, RNA, Messenger, Tumor Cells, Cultured, Umbilical Veins, Down-Regulation, Cell Hypoxia, Endothelial Growth Factors, Vascular Endothelial Growth Factors, Vascular Endothelial Growth Factor A, Rats

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