Cited by 3,605Angiogenesis and VEGF in CancerLymphatic System and DiseasesCancer, Hypoxia, and Metabolism

Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo

Kim, K. Jin, Li, Bing, Winer, Jane, Armanini, Mark, Gillett, Nancy, Phillips, Heidi S., Ferrara, Napoleone·1993·Nature

Abstract

The development of new blood vessels (angiogenesis) is required for many physiological processes including embryogenesis, wound healing and corpus luteum formation. Blood vessel neoformation is also important in the pathogenesis of many disorders, particularly rapid growth and metastasis of solid tumours. There are several potential mediators of tumour angiogenesis, including basic and acidic fibroblast growth factors, tumour necrosis factor-alpha and transforming factors-alpha and -beta. But it is unclear whether any of these agents actually mediates angiogenesis and tumour growth in vivo. Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen and an angiogenesis inducer released by a variety of tumour cells and expressed in human tumours in situ. To test whether VEGF may be a tumour angiogenesis factor in vivo, we injected human rhabdomyosarcoma, glioblastoma multiforme or leiomyosarcoma cell lines into nude mice. We report here that treatment with a monoclonal antibody specific for VEGF inhibited the growth of the tumours, but had no effect on the growth rate of the tumour cells in vitro. The density of vessels was decreased in the antibody-treated tumours. These findings demonstrate that inhibition of the action of an angiogenic factor spontaneously produced by tumour cells may suppress tumour growth in vivo.

Integrity signals

Registry

Crossref · 10.1038/362841a0

Cites

31 of 31 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In 1993, anti-VEGF monoclonal antibodies significantly reduced vessel density and delayed tumor growth in nude mice carrying rhabdomyosarcoma, glioblastoma multiforme, and leiomyosarcoma xenografts.

    “In 1993, it was reported that treatment with anti‐VEGF monoclonal antibodies resulted in a significant reduction in vessel density and a significant delay in tumor growth in nude mice carrying rhabdomyosarcoma, glioblastoma multiforme (GBM), and leiomyosarcoma xenografts.”

Cites

Flagged first, then by citations

1 of the 32 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Kim, K. J., Li, B., Winer, J., Armanini, M., Gillett, N., Phillips, H. S., & Ferrara, N.
Affiliations
Genentech (US)
Venue
Nature
Published
1993-04
Volume
362
Issue
6423
Pages
841-844
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W1969328947
Cited by
3,605
References
32
Citations by year
2026: 22 · 2025: 25 · 2024: 43 · 2023: 47 · 2022: 53 · 2021: 55 · 2020: 55 · 2019: 54 · 2018: 89 · 2017: 101 · 2016: 81 · 2015: 78 · 2014: 98 · 2013: 110 · 2012: 137
FWCI
32.75
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Keywords
vascular endothelial growth factor, angiogenesis, tumor angiogenesis, tumor growth inhibition, monoclonal antibodies, rhabdomyosarcoma, glioblastoma, leiomyosarcoma, tumor vasculature, nude mice, tumour metastasis
MeSH terms
Animals, Antibodies, Monoclonal, Cell Division, Female, Glioblastoma, Humans, Leiomyosarcoma, Lymphokines, Mice, Nude, Neoplasm Transplantation, Neoplasms, Experimental, Neovascularization, Pathologic, Rhabdomyosarcoma, Tumor Cells, Cultured, Endothelial Growth Factors, Vascular Endothelial Growth Factors, Vascular Endothelial Growth Factor A, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review