Upregulation of α‐synuclein by lipopolysaccharide and interleukin‐1 in human macrophages
Abstract
α‐Synuclein was originally identified as the presynaptic nerve terminal protein. Recently, we reported that α‐synuclein is also expressed in cultured human astrocytes and that its levels are increased by stimulation with interleukin‐1β, suggesting that it may be involved in inflammatory processes. We therefore investigated the effect of inflammatory stimuli on α‐synuclein expression in human macrophages. α‐Synuclein mRNA and protein were detected in cultured human macrophages and levels of α‐synuclein protein were increased by stimulation with lipopolysaccharide and interleukin‐1β in a time‐ and concentration‐dependent manner. Immunofluorescent staining showed that α‐synuclein protein was expressed within the cytoplasm and nucleus. Furthermore, α‐synuclein immunoreactivity was present in alveolar macrophages from human lung tissues. These findings suggest that the function of α‐synuclein is not exclusive to the nervous system and that α‐synuclein may play a role in inflammatory processes and immune responses.
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Endotoxin increases α-synuclein production by macrophages and drives α-synuclein fibrillization.
“Endotoxin increases α-synuclein production by macrophages [100] and drives α-synuclein fibrillization [70, 71].”
From The endotoxin hypothesis of neurodegeneration (2019) · cites this paper as [100]
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- Authors
- Tanji, K., Mori, F., Imaizumi, T., Yoshida, H., Matsumiya, T., Tamo, W., Yoshimoto, M., Odagiri, H., Sasaki, M., Takahashi, H., Satoh, K., & Wakabayashi, K.
- Affiliations
- Hirosaki University (JP)
- Taisho Pharmaceutical (Japan) (JP)
- Brain Research Institute, Niigata University (JP)
- Corresponding author
- Tanji, Kunikazu
- ORCID
- Venue
- Pathology International
- Publisher
- Wiley
- Published
- 2002-09
- Volume
- 52
- Issue
- 9
- Pages
- 572-577
- ISSN
- 1320-5463 (ISSN-L)
- 1440-1827
- OpenAlex
- W2050991927
- Cited by
- 55
- References
- 24
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pathology International (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Naito Foundation
- Uehara Memorial Foundation
- Fondation pour la Recherche Médicale
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- alpha-synuclein, lipopolysaccharide, IL-1β, human macrophages, alveolar macrophages, neuroinflammation, gene upregulation, inflammatory stimuli, immune response, subcellular localization
- MeSH terms
- Cells, Cultured, Dose-Response Relationship, Drug, Fluorescent Antibody Technique, Humans, Immunohistochemistry, Interleukin-1, Lipopolysaccharides, Lung, Macrophages, Nerve Tissue Proteins, Proteins, RNA, Messenger, Blotting, Western, Up-Regulation, Macrophages, Alveolar, Synucleins, alpha-Synuclein
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