The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Activity of natural antimicrobial compounds against Escherichia coli and Salmonella enterica serovar Typhimurium
Abstract
AIMS: The objective of this study was to evaluate the inhibitory activity of several natural organic compounds alone or in combination with nisin against Escherichia coli and Salmonella Typhimurium. METHODS AND RESULTS: The minimum inhibitory concentration (MIC) of five natural organic compounds were determined, and the effect of their combinations with nisin was evaluated by the checkerboard assay using the Bioscreen C. As expected, nisin by itself showed no inhibition against either of the Gram-negative bacteria. Thymol was found to be the most effective with the lowest MIC values of 1.0 and 1.2 mmol 1-1 against Salm. Typhimurium and E. coli, respectively. After thymol, the antimicrobial order of the natural organic compounds was carvacrol > eugenol > cinnamic acid > diacetyl. However, the combination of nisin with the natural organic compounds did not result in the enhancement of their antimicrobial activities. On the contrary, combination of nisin with diacetyl against Salm. Typhimurium resulted in an antagonism of diacetyl activity. CONCLUSIONS: While the individual natural organic compounds showed inhibitory activity against the two Gram-negatives, their combinations with nisin showed no improvement of antimicrobial activity. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows the potential of the natural organic compounds to control E. coli and Salm. Typhimurium.
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“Diacetyl has been shown to exert antifungal and antibacterial effects at low pH [167,168].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [168]
Citations
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BackgroundActivity and mechanisms of action of selected biocidal agents on Gram-positive and-negative bacteria
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Items 201–218 of 218
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Gilling, D. H. (2011) · UA Campus Repository (The University of Arizona)
Lima, J. P. (2007) · doi:10.11606/t.9.2007.tde-15022009-210130
“A nisina e os óleos essenciais agem sobre a membrana citoplasmática e um efeito aditivo ou sinérgico pode ser esperado, permitindo aplicação de ambos antimicrobianos em menor teor sem diminuir o efeito inibitório (NAZER et al., 2005; OLASUPO et al., 2003).”
BackgroundRomano, D. L. (2008) · VTechWorks (Virginia Tech)
Passos, J. A. S., da Silva, L., Célia, R., & Freitas‐Silva, O. (2016)
Hayouni, E. A., Bouix, M., Brahim, N. B., Leveau, J.-Y., & Hamdi, M. (2009)
Antibacterial Potential of Essential Oils Against Ground Beef Isolates
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Kumar, P., Mohapatra, P., Chanotiya, C. S., Bawitlung, L., Pal, A., Bhargava, S. K., Periasamy, S., Sabri, Y. M., Semwal, M., & Rout, P. K. (2025) · Applied Catalysis O Open · doi:10.1016/j.apcato.2025.207053
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“This selectivity may also be a result of the distinctions in cell wall structures between Gram-positive and Gram-negative bacteria, reflecting variations in cell surface [52] and the outer membrane structure [53].”
The rapid analysis of fungal growth in the presence of inhibitory effects
Williams, T. (2011) · CERES (Cranfield University)
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den Hertog, W. E. (2009) · Utrecht University Repository (Utrecht University)
Metadata
- Authors
- Olasupo, N. A., Fitzgerald, D. J., Gasson, M. J., & Narbad, A.
- Affiliations
- Lagos State University (NG)
- Norwich Research Park (GB)
- Corresponding author
- Narbad, A.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-12
- Volume
- 37
- Issue
- 6
- Pages
- 448-451
- ISSN
- 0266-8254 (ISSN-L)
- 1365-2673
- OpenAlex
- W1970033253
- Cited by
- 218
- References
- 22
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 7 · 2022: 11 · 2021: 19 · 2020: 11 · 2019: 12 · 2018: 16 · 2017: 13 · 2016: 8 · 2015: 9 · 2014: 10 · 2013: 12 · 2012: 12
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- natural antimicrobials, Escherichia coli, Salmonella typhimurium, nisin, thymol, carvacrol, eugenol, cinnamic acid, diacetyl, minimum inhibitory concentration, synergistic and antagonistic effects, Gram-negative bacteria, food safety
- MeSH terms
- Cymenes, Anti-Bacterial Agents, Cinnamates, Diacetyl, Drug Therapy, Combination, Escherichia coli, Eugenol, Microbial Sensitivity Tests, Nisin, Salmonella typhimurium, Thymol, Monoterpenes, In Vitro Techniques
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