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Involvement of Human Micro-RNA in Growth and Response to Chemotherapy in Human Cholangiocarcinoma Cell Lines
Open accessAbstract
Background & aims Micro-RNA (miRNA) are endogenous regulatory RNA molecules that modulate gene expression. Alterations in miRNA expression can contribute to tumor growth by modulating the functional expression of critical genes involved in tumor cell proliferation or survival. Our aims were to identify specific miRNA involved in the regulation of cholangiocarcinoma growth and response to chemotherapy. Methods miRNA expression in malignant and nonmalignant human cholangiocytes was assessed using a microarray. Expression of selected miRNA and their precursors was evaluated by Northern blots and real-time polymerase chain reaction, respectively. The effect of selected miRNA on cell growth and response to chemotherapy was assessed using miRNA-specific antisense oligonucleotides to decrease miRNA expression or with precursor miRNA to increase cellular expression. Results miRNA expression was markedly different in malignant cholangiocytes, with decreased expression of many miRNA compared with nonmalignant cells. A cluster of miRNA, including miR-320, miR-200b, miR-21, miR-23a, miR-141, miR-27a, and miR-34a, were expressed in all cell lines. MiR-21, miR-141, and miR-200b were highly over-expressed in malignant cholangiocytes. Inhibition of miR-21 and miR-200b increased sensitivity to gemcitabine, whereas inhibition of miR-141 decreased cell growth. Treatment of tumor cell xenografts with systemic gemcitabine altered the expression of a significant number of miRNA. miR-21 modulates gemcitabine-induced apoptosis by phosphatase and tensin homolog deleted on chromosome 10 (PTEN)-dependent activation of PI 3-kinase signaling. Potential target genes that were modulated by selected miRNA were identified. Conclusions Alterations in miRNA expression contribute to tumor growth and response to chemotherapy. Aberrantly expressed miRNA or their targets will provide mechanistic insight and therapeutic targets for cholangiocarcinoma.
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“Three miR-21 targets have, thus far, been described: phosphatase and tensin homologue (PTEN), programmed cell death 4 (PDCD4) and Tropomyosin 1 (TPM1; refs.10,[14][15][16].”
From MicroRNA-21 Targets a Network of Key Tumor-Suppressive Pathways in Glioblastoma Cells (2008) · cites this paper as [10]
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Cites
MicroRNA expression profiles classify human cancers
Lü, J., Getz, G. A., Miska, E. A., Alvarez-Saavedra, E., Lamb, J., Peck, D., Sweet-Cordero, A., Ebert, B. L., Mak, R. H., Ferrando, A. A., et al. (2005) · Nature · doi:10.1038/nature03702 · Cited by 9,637
Calin, G. A., Dumitru, C. D., Shimizu, M., Bichi, R., Zupo, S., Noch, E., Aldler, H., Rattan, S., Keating, M., Rai, K., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.242606799 · Cited by 5,111
TM4: a free, open-source system for microarray data management and analysis
Saeed, A. I., Sharov, V., White, J., Li, J., Liang, W., Bhagabati, N., Braisted, J., Klapa, M., Currier, T., Thiagarajan, M., et al. (2003) · BioTechniques · doi:10.2144/03342mt01 · Cited by 4,837
Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers
Calin, G. A., Sevignani, C., Dumitru, C. D., Hyslop, T., Noch, E., Yendamuri, S., Shimizu, M., Rattan, S., Bullrich, F., Negrini, M., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307323101 · Cited by 4,096
A microRNA polycistron as a potential human oncogene
He, L., Thomson, J. M., Hemann, M. T., Hernando, E., Mu, D., Goodson, S. G., Powers, S., Cordon‐Cardo, C., Lowe, S. W., Hannon, G. J., et al. (2005) · Nature · doi:10.1038/nature03552 · Cited by 3,642
An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans
Lau, N. C., Lim, L. P., Weinstein, E. G., & Bartel, D. P. (2001) · Science · doi:10.1126/science.1065062 · Cited by 3,345
microRNAs as oncogenes and tumor suppressors
Zhang, B., Pan, X., Cobb, G. P., & Anderson, T. A. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.08.028 · Cited by 2,782
Modulation of Hepatitis C Virus RNA Abundance by a Liver-Specific MicroRNA
Jopling, C. L., Yi, M., Lancaster, A. M., Lemon, S. M., & Sarnow, P. (2005) · Science · doi:10.1126/science.1113329 · Cited by 2,580
MicroRNA-21 Is an Antiapoptotic Factor in Human Glioblastoma Cells
Chan, J. A., Krichevsky, A. M., & Kosik, K. S. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-0137 · Cited by 2,557
c-Myc-regulated microRNAs modulate E2F1 expression
O'Donnell, K. A., Wentzel, E. A., Zeller, K. I., Dang, C. V., & Mendell, J. T. (2005) · Nature · doi:10.1038/nature03677 · Cited by 2,347
Self-citationSpecificity of microRNA target selection in translational repressionPreprint
Doench, J. G., & Sharp, P. A. (2004) · Genes & Development · doi:10.1101/gad.1184404 · Cited by 1,685
Reduced accumulation of specific microRNAs in colorectal neoplasia.
Michael, M., Connor, S. M. O., Pellekaan, N. V. H., Young, G. P., & James, R. J. (2003) · PubMed · Cited by 1,576
MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias
Calin, G. A., Liu, C., Sevignani, C., Ferracin, M., Felli, N., Dumitru, C. D., Shimizu, M., Cimmino, A., Zupo, S., Dono, M., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404432101 · Cited by 1,279
The Multiple Roles of PTEN in Tumor Suppression
Di Cristofano, A., & Pandolfi, P. P. (2000) · Cell · doi:10.1016/s0092-8674(00)80674-1 · Cited by 1,182
Khan, S. A., Thomas, H. C., Davidson, B. R., & Taylor-Robinson, S. D. (2005) · The Lancet · doi:10.1016/s0140-6736(05)67530-7 · Cited by 1,064
Increasing Incidence and Mortality of Primary Intrahepatic Cholangiocarcinoma in the United States
Patel, T. P. (2001) · Hepatology · doi:10.1053/jhep.2001.25087 · Cited by 1,010
Self-citationAn oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
Liu, C.-G., Calin, G. A., Meloon, B., Gamliel, N., Sevignani, C., Ferracin, M., Dumitru, C. D., Shimizu, M., Zupo, S., Dono, M., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0403293101 · Cited by 948
A combined computational-experimental approach predicts human microRNA targetsPreprint
Kiriakidou, M., Nelson, P. T., Kouranov, A., Fitziev, P., Bouyioukos, C., Mourelatos, Z., & Hatzigeorgiou, A. G. (2004) · Genes & Development · doi:10.1101/gad.1184704 · Cited by 774
MicroRNAs as Oncogenes and Tumor Suppressors
Chen, C. (2005) · New England Journal of Medicine · doi:10.1056/nejmp058190 · Cited by 760
High expression of precursor microRNA‐155/BIC RNA in children with Burkitt lymphoma
Metzler, M., Wilda, M., Busch, K., Viehmann, S. F., & Borkhardt, A. (2003) · Genes Chromosomes and Cancer · doi:10.1002/gcc.10316 · Cited by 543
Real-time expression profiling of microRNA precursors in human cancer cell lines
Jiang, J. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki863 · Cited by 501
Self-citationWorldwide trends in mortality from biliary tract malignancies
Patel, T. C. (2002) · BMC Cancer · doi:10.1186/1471-2407-2-10 · Cited by 491
Self-citationA high-throughput method to monitor the expression of microRNA precursors
Schmittgen, T. D. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh040 · Cited by 484
Self-citationMcManus, M. T. (2003) · Seminars in Cancer Biology · doi:10.1016/s1044-579x(03)00038-5 · Cited by 441
Lazaridis, K. N., & Gores, G. J. (2005) · Gastroenterology · doi:10.1053/j.gastro.2005.03.040 · Cited by 395
Increase in mortality rates from intrahepatic cholangiocarcinoma in England and Wales 1968–1998
Taylor-Robinson, S. D., Toledano, M. B., Arora, S., Keegan, T., Hargreaves, S., Beck, A. J., Khan, S. A., Elliott, P., & Thomas, H. C. (2001) · Gut · doi:10.1136/gut.48.6.816 · Cited by 380
Liang, R. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni019 · Cited by 331
Patel, T. C. (2006) · Nature Clinical Practice Gastroenterology & Hepatology · doi:10.1038/ncpgasthep0389 · Cited by 228
Self-citationPark, J., Tadlock, L., Gores, G. J., & Patel, T. C. (1999) · Hepatology · doi:10.1002/hep.510300522 · Cited by 206
Self-citationProgenetix.net: an online repository for molecular cytogenetic aberration data
Baudis, M., & Cleary, M. L. (2001) · Bioinformatics · doi:10.1093/bioinformatics/17.12.1228 · Cited by 190
Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning
Ambros, V., & Lee, R. C. (2004) · Humana Press eBooks · doi:10.1385/1-59259-775-0:131 · Cited by 186
Regulation of intracellular pH by immortalized human intrahepatic biliary epithelial cell lines
Grubman, S. A., Perrone, R. D., Lee, D. W., Murray, S., Rogers, L. C., Wolkoff, L. I., Mulberg, A. E., Cherington, V., & Jefferson, D. M. (1994) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1994.266.6.g1060 · Cited by 185
Lee, J. T., Steelman, L. S., & McCubrey, J. A. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-1612 · Cited by 156
microRNA-guided posttranscriptional gene regulation
Chen, P., & Meister, G. (2005) · Biological Chemistry · doi:10.1515/bc.2005.139 · Cited by 136
Kandasamy, K., & Srivastava, R. K. (2002) · PubMed · Cited by 131
Cong, F., Spencer, S. D., Côté, J., Wu, Y. L., Tremblay, M. L., Lasky, L. A., & Goff, S. P. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00138-6 · Cited by 128
Park, J., Gores, G. J., & Patel, T. C. (1999) · Hepatology · doi:10.1002/hep.510290423 · Cited by 123
Self-citationDavidson, D., & Veillette, A. (2001) · The EMBO Journal · doi:10.1093/emboj/20.13.3414 · Cited by 118
Yamagiwa, Y., Marienfeld, C. B., Tadlock, L., & Patel, T. C. (2003) · Hepatology · doi:10.1053/jhep.2003.50257 · Cited by 34
Self-citationThe age of high-throughput microRNA profiling
Esquela‐Kerscher, A., & Slack, F. J. (2004) · Nature Methods · doi:10.1038/nmeth1104-106 · Cited by 19
Double-stranded RNA activates a p38 MAPK-dependent cell survival program in biliary epithelia
Tadlock, L., Yamagiwa, Y., Marienfeld, C. B., & Patel, T. C. (2003) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00355.2002 · Cited by 15
Self-citationChariyalertsak, S., Khuhaprema, T., Bhudisawasdi, V., Sripa, B., Wongkham, S., & Petmitr, S. (2005) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-005-0031-2 · Cited by 12
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Sorted by citations
MicroRNA signatures in human cancers
Calin, G. A., & Croce, C. M. (2006) · Nature reviews. Cancer · doi:10.1038/nrc1997 · Cited by 7,668
MicroRNA-21 Regulates Expression of the PTEN Tumor Suppressor Gene in Human Hepatocellular Cancer
Meng, F., Henson, R., Wehbe–Janek, H., Ghoshal, K., Jacob, S. T., & Patel, T. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.05.022 · Cited by 2,773
Self-citationA coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Poliseno, L., Salmena, L., Zhang, J., Carver, B. S., Haveman, W. J., & Pandolfi, P. P. (2010) · Nature · doi:10.1038/nature09144 · Cited by 2,364
The functions and regulation of the PTEN tumour suppressor
Song, M. S., Salmena, L., & Pandolfi, P. P. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3330 · Cited by 1,926
Asangani, I. A., Rasheed, S. A. K., Nikolova, D. A., Leupold, J. H., Colburn, N. H., Post, S., & Allgayer, H. (2008) · Oncogene · doi:10.1038/sj.onc.1210856 · Cited by 1,882
Metadata
- Authors
- Meng, F., Henson, R., Lang, M., Wehbe, H., Maheshwari, S., Mendell, J. T., Jiang, J., Schmittgen, T. D., & Patel, T.
- Affiliations
- Texas A&M University System (US)
- Johns Hopkins University (US)
- The Ohio State University (US)
- Corresponding author
- Patel, Tushar
- Venue
- Gastroenterology
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 130
- Issue
- 7
- Pages
- 2113-2129
- ISSN
- 0016-5085 (ISSN-L)
- OpenAlex
- W1990645475
- Cited by
- 984
- References
- 44
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 13 · 2023: 24 · 2022: 26 · 2021: 27 · 2020: 22 · 2019: 28 · 2018: 27 · 2017: 49 · 2016: 60 · 2015: 64 · 2014: 86 · 2013: 76 · 2012: 65
- FWCI
- 27.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gastroenterology (publishedVersion)
- PubMed (publishedVersion)
- OakTrust (Texas A&M University Libraries) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-069370, R01DK069370)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Surgery › Cholangiocarcinoma and Gallbladder Cancer Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- microRNA, cholangiocarcinoma, miR-21, miR-200b, miR-141, gemcitabine, chemotherapy response, tumor cell proliferation, PTEN, PI3K signaling, apoptosis, miRNA expression profiling, cholangiocytes, xenografts, therapeutic target
- MeSH terms
- Gemcitabine, Animals, Deoxycytidine, Humans, Male, Mice, Nude, Probability, Sampling Studies, Sensitivity and Specificity, Transfection, Transplantation, Heterologous, Blotting, Northern, Blotting, Western, Gene Expression Regulation, Neoplastic, Apoptosis, Cholangiocarcinoma, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Cell Line, Tumor, Cell Proliferation, Mice
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