Cited by 195Osteoarthritis Treatment and MechanismsEstrogen and related hormone effectsEffects and risks of endocrine disrupting chemicals

Expression of genes for estrogen receptors α and β in human articular chondrocytes

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Ushiyama, T., Ueyama, H., Inoue, K., Ohkubo, I., Hukuda, S.·1999·Osteoarthritis and Cartilage

Abstract

Objective To investigate the gene expression of estrogen receptor (ER) alpha and ERbeta in human articular chondrocytes.Methods 16 articular cartilage specimens were obtained from 15 patients during surgery. Three of the specimens were from men and 13 from women; three from hip joints and 13 from knee joints; four were normal and 12 showed osteoarthritic cartilage. Total RNA was extracted from the articular chondrocytes and the expression of both ERalpha and ERbeta genes was investigated by the reverse transcription-polymerase chain reaction (RT-PCR) method.Results Gene expressions of ERalpha were detected in all specimens and those of ERbeta were found in 15 specimens by the RT-PCR method. There was a significant correlation between the amounts of ERalpha and ERbeta. Expression levels of both genes were significantly higher in men than in women. There were no significant differences in the expression levels of both ER genes between the hip and knee joint sites, nor between normal and osteoarthritic tissues.Conclusion This study is to our knowledge the first to demonstrate the gene expression of both ERalpha and ERbeta in human articular chondrocytes. Since there are some functional differences between the two receptors, the effects of estrogen on cartilage metabolism should be elucidated by two different receptor mechanisms.

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Crossref · 10.1053/joca.1999.0260

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  • Circulating estrogens act on estrogen receptors expressed by auricular chondrocytes to elevate hyaluronic acid levels and pliability of the ear cartilage.

    “These circulating estrogens act on estrogen receptors expressed by auricular chondrocytes to elevate hyaluronic acid levels and pliability of the ear cartilage.4–6”

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Authors
Ushiyama, T., Ueyama, H., Inoue, K., Ohkubo, I., & Hukuda, S.
Affiliations
Shiga University of Medical Science (JP)
Venue
Osteoarthritis and Cartilage
Publisher
Elsevier BV
Published
1999-11
Volume
7
Issue
6
Pages
560-566
ISSN
1063-4584 (ISSN-L)
OpenAlex
W2023917616
Cited by
195
References
42
Citations by year
2026: 4 · 2025: 6 · 2024: 8 · 2023: 9 · 2022: 5 · 2021: 4 · 2020: 8 · 2019: 7 · 2018: 7 · 2017: 10 · 2016: 8 · 2015: 7 · 2014: 4 · 2013: 11 · 2012: 13
FWCI
0.85
Type
journal article
Language
en
Open access
Locations
  • Osteoarthritis and Cartilage (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
Keywords
estrogen receptor alpha, estrogen receptor beta, articular chondrocytes, articular cartilage, gene expression, osteoarthritis, sex differences
MeSH terms
Adult, Aged, Cartilage, Articular, Female, Humans, Male, Middle Aged, Osteoarthritis, Receptors, Estrogen, RNA, Messenger, Sex Factors, Gene Expression, Chondrocytes, Reverse Transcriptase Polymerase Chain Reaction, Estrogen Receptor alpha, Estrogen Receptor beta

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