Expression of genes for estrogen receptors α and β in human articular chondrocytes
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Objective To investigate the gene expression of estrogen receptor (ER) alpha and ERbeta in human articular chondrocytes.Methods 16 articular cartilage specimens were obtained from 15 patients during surgery. Three of the specimens were from men and 13 from women; three from hip joints and 13 from knee joints; four were normal and 12 showed osteoarthritic cartilage. Total RNA was extracted from the articular chondrocytes and the expression of both ERalpha and ERbeta genes was investigated by the reverse transcription-polymerase chain reaction (RT-PCR) method.Results Gene expressions of ERalpha were detected in all specimens and those of ERbeta were found in 15 specimens by the RT-PCR method. There was a significant correlation between the amounts of ERalpha and ERbeta. Expression levels of both genes were significantly higher in men than in women. There were no significant differences in the expression levels of both ER genes between the hip and knee joint sites, nor between normal and osteoarthritic tissues.Conclusion This study is to our knowledge the first to demonstrate the gene expression of both ERalpha and ERbeta in human articular chondrocytes. Since there are some functional differences between the two receptors, the effects of estrogen on cartilage metabolism should be elucidated by two different receptor mechanisms.
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“These circulating estrogens act on estrogen receptors expressed by auricular chondrocytes to elevate hyaluronic acid levels and pliability of the ear cartilage.4–6”
From A Retrospective Review of Outcomes and Complications after Infant Ear Molding at a Single Institution (2023) · cites this paper as [4]
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Chan, S. L. S., Lim, G. J. S., Por, Y. C., Chiang, M. F., Ho, S., Saffari, S. E., & Chia, H.-L. (2019) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0000000000006057 · Cited by 45 · see citing works
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- Authors
- Ushiyama, T., Ueyama, H., Inoue, K., Ohkubo, I., & Hukuda, S.
- Affiliations
- Shiga University of Medical Science (JP)
- Venue
- Osteoarthritis and Cartilage
- Publisher
- Elsevier BV
- Published
- 1999-11
- Volume
- 7
- Issue
- 6
- Pages
- 560-566
- ISSN
- 1063-4584 (ISSN-L)
- OpenAlex
- W2023917616
- Cited by
- 195
- References
- 42
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 8 · 2023: 9 · 2022: 5 · 2021: 4 · 2020: 8 · 2019: 7 · 2018: 7 · 2017: 10 · 2016: 8 · 2015: 7 · 2014: 4 · 2013: 11 · 2012: 13
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Osteoarthritis and Cartilage (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Keywords
- estrogen receptor alpha, estrogen receptor beta, articular chondrocytes, articular cartilage, gene expression, osteoarthritis, sex differences
- MeSH terms
- Adult, Aged, Cartilage, Articular, Female, Humans, Male, Middle Aged, Osteoarthritis, Receptors, Estrogen, RNA, Messenger, Sex Factors, Gene Expression, Chondrocytes, Reverse Transcriptase Polymerase Chain Reaction, Estrogen Receptor alpha, Estrogen Receptor beta
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