Two Flavonols from Artemisa annua which Potentiate the Activity of Berberine and Norfloxacin Against a Resistant Strain of Staphylococcus aureus
Abstract
Bioassay-guided fractionation of an extract of Artemisia annua L. (Asteraceae) was conducted in order to assess the possible presence in the plant material of inhibitors of bacterial multidrug resistance pumps. Fractions were tested for Staphylococcus aureus growth inhibition in the presence of a subinhibitory dose of the weak antibacterial alkaloid berberine. Active fractions yielded the flavones chrysosplenol-D and chrysoplenetin, which themselves had very weak growth inhibitory action, but which made a potent combination with berberine. In comparison with work on other flavonols, it is likely that potentiation is due to the inhibition of an S. aureus multidrug resistance (MDR) pump. These same two flavonols were earlier reported to potentiate the activity of artemisinin against Plasmodium falciparum.
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The more abundant methoxylated flavonoids from A. annua include casticin (227), artemetin (228), chrysosplenol D (225) and chrysoplenetin (226).
“These flavones include: casticin (227), artemetin (228) [91], chrysosplenol D (225) and chrysoplenetin (226) [92]”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [92]
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- Authors
- Stermitz, F. R., Scriven, L. N., Tegos, G., & Lewis, K.
- Affiliations
- Colorado State University (US)
- Northeastern University (US)
- Venue
- Planta Medica
- Publisher
- Georg Thieme Verlag KG
- Published
- 2002-12
- Volume
- 68
- Issue
- 12
- Pages
- 1140-1141
- ISSN
- 0032-0943 (ISSN-L)
- 1439-0221
- OpenAlex
- W2039587632
- Cited by
- 114
- References
- 0
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 10 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 9
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Planta Medica (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Colorado State University
- National Institutes of Health (5r01gm059903-03)
- National Institute of General Medical Sciences (R01 GM 59903)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- Artemisia annua, flavonols, berberine, norfloxacin, Staphylococcus aureus, multidrug resistance, efflux pump inhibitors, bioassay-guided fractionation
- MeSH terms
- Anti-Infective Agents, Berberine, Drug Synergism, Flavonoids, Norfloxacin, Plant Extracts, Staphylococcus aureus, Molecular Structure, Drug Resistance, Bacterial, Artemisia annua
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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