Postprandial Mononuclear NF-κB Activation is Independent of the AGE-content of a Single Meal
Abstract
OBJECTIVE: Dietary uptake of Advanced Glycation Endproducts (AGE) is supposed to potentially contribute to inflammatory reactions linked to vascular dysfunction and late diabetic complications. One mechanism by which dietary AGE might exert these effects is by activation of the proinflammatory transcription factor NF-kappa-B. The aim of this study was to analyze the postprandial effects of a casein meal with low or high AGE content on postprandial NF-kappaB activation in peripheral blood mononuclear cells (pBMC) of healthy volunteers. RESEARCH DESIGN AND METHODS: Casein was heated for 40 h at 50 degrees C in the presence of sorbitol or glucose, resulting in either minimal (Sorbitol [S]-casein) or large (glucose [G]-casein) amounts of AGE-modified casein. Nine healthy volunteers ate 250 g of both types of casein, whereas both meals were separated at least by 2 weeks. Plasma and pBMC were taken before and 2 h after each meal. Thereafter, the defined AGE carboxymethyllysine (CML) was determined by ELISA and Western blot. NF-kappaB activation in pBMC was assayed using Electrophoretic Mobility Shift Assays (EMSA) and Western blot analysis. RESULTS: S-casein contained only minor amounts of CML and no pentosidine, while G-casein contained large amounts of both. 2 h after ingestion, the S-casein or the G-casein-meal, both, resulted in a non-significant increase in plasma CML and in the intracellular CML-content of pBMC. This was paralleled by a highly significant increase in postprandial mononuclear NF-kappaB-binding activity. Remarkably, neither the extent of NF-kappaB induction (178% for S-casein, 188% for G-casein), nor composition of the NF-kappaB heterodimer (mainly consisting of NF-kappaB p50/p65) were significantly different after intake of S-casein or G-casein. Consistently, Western blots confirmed an increased NF-kappaBp65 nuclear translocation and a decrease of NF-kappaBp65 in the cytoplasm, while no difference in postprandial NF-kappaB nuclear translocation was observed following intake of S-casein or G-casein. CONCLUSION: Postprandial mononuclear NF-kappaB activation after a single meal is independent of the AGE-content of the ingested protein.
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After adjusting for body fat, no associations between cardiovascular fitness or physical activity and inflammatory markers were observed in 9-year-old Swedish children.
“Nevertheless, once body fat was entered into the regression models, no associations with cardiovascular fitness or physical activity and the inflammatory markers measured were observed (257) .”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [257]
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- Authors
- Schiekofer, S., Franke, Andrassy, M., Chen, Rudofsky, G., Schneider, von Eynatten, M., Wendt, Morcos, M., Kientsch-Engel, Stein, G., Schleicher, Nawroth, P., & Bierhaus, A.
- Affiliations
- Heidelberg University (DE)
- Baylor College of Medicine (US)
- Friedrich Schiller University Jena (DE)
- University of Tübingen (DE)
- Venue
- Experimental and Clinical Endocrinology & Diabetes
- Publisher
- Georg Thieme Verlag KG
- Published
- 2006-05-17
- Volume
- 114
- Issue
- 04
- Pages
- 160-167
- ISSN
- 0947-7349 (ISSN-L)
- 1439-3646
- OpenAlex
- W2018658316
- Cited by
- 19
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 3
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental and Clinical Endocrinology & Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Eli Lilly and Company
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Keywords
- advanced glycation end products, NF-κB activation, postprandial inflammation, peripheral blood mononuclear cells, casein, carboxymethyllysine, pentosidine, p65 nuclear translocation, sorbitol, glucose, diabetes complications, healthy volunteers
- MeSH terms
- Administration, Oral, Caseins, Cell Nucleus, Food, Formulated, Humans, Leukocytes, Mononuclear, Lysine, Male, Vascular Diseases, NF-kappa B, Glycation End Products, Advanced, Active Transport, Cell Nucleus, Diabetes Complications
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