In vitro Acetylcholinesterase Inhibitory Activity of the Essential Oil from Acorus calamus and its Main Constituents

Mukherjee, Pulok; Kumar, Venkatesan; Mal, Mainak; Houghton, Peter

doi:10.1055/s-2007-967114

Cited by 136Cholinesterase and Neurodegenerative DiseasesEssential Oils and Antimicrobial ActivityMedicinal Plants and Neuroprotection

In vitro Acetylcholinesterase Inhibitory Activity of the Essential Oil from Acorus calamus and its Main Constituents

Mukherjee, Pulok, Kumar, Venkatesan, Mal, Mainak, Houghton, Peter·2007·Planta Medica

DOI 10.1055/s-2007-967114

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Abstract

The in vitro acetylcholinesterase (AChE) inhibitory potential of the hydroalcoholic extract and of the essential oil from Acorus calamus (AC) rhizomes and that of its major constituents were evaluated based on the Ellman's method. GC/MS analysis of the oil revealed that the major constituents were beta-asarone (79.54%) and alpha-asarone (8.47%). The IC50 values were obtained for the hydroalcoholic extract, the essential oil, beta-asarone and alpha-asarone and were 182.31+/-16.78 microg/mL, 10.67+/-0.81 microg/mL, 3.33+/-0.02 microM and 46.38+/-2.69 microM, respectively. Physostigmine was used as standard inhibitor with an IC50 value of 0.28+/-0.015 microM. The experimental observations revealed that the AC essential oil and its constituents have significant AChE inhibitory potential. beta-Asarone, the major phytoconstituent present in the essential oil, showed the maximum inhibitory potential.

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β-asarone showed maximum inhibition against AChE among Acorus calamus constituents.
“The β-asarone showed the maximum inhibition against AChE [95].”
From Acetylcholinesterase Inhibitory Potential of Various Sesquiterpene Analogues for Alzheimer’s Disease Therapy (2021) · cites this paper as [95]

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“the inhibitory activity of ache activity was assessed in brain tissues as described previously [51, 52]. the absorbance of the inhibitory activity of ache against enzyme and time was plotted. using the slope of the line obtained the activity was calculated as a percentage in comparison to an assay…”
Method

Authors: Mukherjee, P., Kumar, V., Mal, M., & Houghton, P.
Affiliations: Jadavpur University (IN)
King's College London (GB)
ORCID: Mukherjee, Pulok
Mal, Mainak
Houghton, Peter
Venue: Planta Medica
Publisher: Georg Thieme Verlag KG
Published: 2007-03
Volume: 73
Issue: 3
Pages: 283-285
ISSN: 0032-0943 (ISSN-L)
1439-0221
DOI: 10.1055/s-2007-967114
OpenAlex: W2086672378
Cited by: 136
References: 0
Citations by year: 2026: 3 · 2025: 6 · 2024: 5 · 2023: 5 · 2022: 7 · 2021: 12 · 2020: 11 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 9 · 2015: 8 · 2014: 5 · 2013: 6 · 2012: 11
FWCI: 4.68
Type: journal article
Language: en
Open access: closed
Locations: Planta Medica (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
Keywords: acetylcholinesterase inhibition, Acorus calamus, essential oils, β-asarone, α-asarone, hydroalcoholic extract, rhizome, IC50, physostigmine, Ellman's method, gas chromatography-mass spectrometry
MeSH terms: Allylbenzene Derivatives, Anisoles, Cholinesterase Inhibitors, Humans, Phytotherapy, Plant Oils, Inhibitory Concentration 50, Rhizome, Acorus

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In vitro Acetylcholinesterase Inhibitory Activity of the Essential Oil from Acorus calamus and its Main Constituents

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