Effect of culture conditions on the growth rate and lipid production of microalgae Nannochloropsis gaditana
Abstract
Microalgae Nannochloropsis gaditana (CCMP527) is an ideal candidate as a renewable and sustainable alternative oil source to traditional fossil fuel. It has advantages including high oil content, short growth cycle, and high biomass yield. Experimental data showed that with 5% CO2 enriched air and tris-HCl buffered f/2-Si medium, a maximum growth rate of 0.48 day−1 and a lipid yield of 0.32 g/l were achieved. Various carbon and nitrogen sources were investigated to determine the range of substrates that may be feasible for cultivation with minimal impact on productivity. The sources evaluated cover some of the compounds found in fertilizers and groundwater. When glucose was added into the medium, microalgae grew faster mixotrophically but the lipid yield was reduced by 86%. Adding high concentrations of nitrogen, 10 mM N in the form of nitrate, ammonium sulfate, glycine, and urea, resulted in variations in lipid yield from 0.02 g/l to 0.27 g/l. However, the growth rate increased slightly to 0.52 day−1 at the higher nitrogen concentration when nitrate and urea were the nitrogen sources. The major fatty acids were C16:0 and C16:1 regardless of the carbon and nitrogen sources, which are readily converted to fuels. While the amount of nitrogen affects productivity, the types of fatty acids produced are not affected hence making cultivation on impaired waters feasible.
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Research confirms the high lipid accumulation potential of Nannochloropsis gaditana (e.g., reaching 0.32 g/L lipid yield under specific enriched CO2 lab conditions) and the focus of genetic engineering on optimizing these metabolic pathways, while specific quantitative comparisons of CO2-to-lipid conversion efficiencies between genetically modified strains and wild-type counterparts require进一步整合数据
“While specific quantitative comparisons of CO 2 -to-lipid conversion efficiencies between genetically modified strains (e.g., Nannochloropsis gaditana) and their wild-type counterparts require further consolidated data, research confirms the high lipid accumulation potential of species like N. gaditana (e.g., reaching 0.32 g/L lipid yield under specific enriched CO 2 lab conditions) and the focus”
From Biofuels, E-Fuels, and Waste-Derived Fuels: Advances, Challenges, and Future Directions (2025) · cites this paper as [167]
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- Authors
- Ren, M., Ogden, K., & Lian, B.
- Affiliations
- University of Arizona (US)
- ORCID
- Ogden, Kimberly
- Venue
- Journal of Renewable and Sustainable Energy
- Publisher
- AIP Publishing
- Published
- 2013-11-01
- Volume
- 5
- Issue
- 6
- ISSN
- 1941-7012 (ISSN-L)
- OpenAlex
- W2084435632
- Cited by
- 21
- References
- 36
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 3 · 2015: 3 · 2014: 3
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Renewable and Sustainable Energy (publishedVersion)
- Funders
- DOE (DE-EE0003046)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Nannochloropsis gaditana, microalgae cultivation, lipid production, growth rate, carbon source, nitrogen source, elevated CO2, mixotrophic growth, glucose, fatty acid composition, C16:0, biofuel feedstock, nitrogen fertilization, groundwater
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