Evaluation of selected angiogenic and inflammatory markers in endometriosis before and after danazol treatment
Abstract
Angiogenesis and inflammation are pivotal processes in developing endometriosis in the peritoneal cavity. The aim of the present study was to evaluate these two processes in women with endometriosis who had been treated with danazol to determine the sensitivity of a non-invasive test in diagnosing endometriosis. The clinical follow-up study was conducted in a group of 103 women diagnosed laparoscopically with endometriosis. Thirty-five patients qualified for danazol treatment. Pain was assessed using a visual analogue scale, whereas endometriosis was assessed using the revised American Society of Reproductive Medicine (rASRM) scale. Cancer antigen (CA)-125 and C-reactive protein (CRP) concentrations in plasma and peritoneal fluid were determined by immunoenzymatic methods, whereas vascular endothelial growth factor (VEGF) and interleukin (IL)-1ß concentrations in plasma and peritoneal fluid were determined by ELISA. Endometrial expression of IL-8 and platelet-derived growth factor alpha polypeptide (PDGF-A) was determined using real-time polymerase chain reaction (PCR). Women with endometriosis (68.9% of patients) had higher plasma concentrations of CA-125, as well as higher concentrations of both CA-125 and VEGF in the peritoneal fluid. Endometrial expression of IL-8 mRNA was significantly higher, whereas that of PDGF-A was significantly lower, in contrast. After danazol treatment the patients reported lower pain scores; in addition, CA-125 concentrations in the plasma were decreased (P < 0.001), whereas VEGF concentration in the plasma increased (P = 0.009). For the diagnosis of endometriosis, none of the combinations of given markers had a sensitivity >60%. Danazol treatment is highly effective in relieving pain and decreasing CA-125 concentrations in the plasma. Higher plasma concentrations of VEGF after treatment could imply stimulation of angiogenesis.
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Danazol treatment of endometriosis patients resulted in an increased VEGF concentration in plasma.
“In another study, danazol treatment of endometriosis patients resulted in an increased VEGF concentration in plasma [70].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [70]
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- Authors
- Szubert, M., Suzin, J., Duechler, M., Szulawska, A., Czyz, M., & Kowalczyk-Amico, K.
- Affiliations
- Medical University of Lodz (PL)
- Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk (PL)
- CDepartment of Molecular Biology of Cancer of Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
- Corresponding author
- Szubert, M.
- Venue
- Reproduction, Fertility and Development
- Publisher
- CSIRO Publishing
- Published
- 2013-04-02
- Volume
- 26
- Issue
- 3
- Pages
- 414-420
- ISSN
- 1031-3613 (ISSN-L)
- 1448-5990
- OpenAlex
- W2103648079
- Cited by
- 36
- References
- 37
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 2 · 2020: 4 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 1
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Reproduction Fertility and Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundacja na rzecz Nauki Polskiej
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
- Keywords
- endometriosis, danazol, angiogenesis, inflammation, CA125, vascular endothelial growth factor, C-reactive protein, IL-1β, interleukin-8, PDGF-A, endometrial gene expression, endometriosis-associated pain, non-invasive diagnosis, treatment response
- MeSH terms
- Ascitic Fluid, C-Reactive Protein, Danazol, Endometriosis, Enzyme-Linked Immunosorbent Assay, Female, Follow-Up Studies, Humans, Immunoenzyme Techniques, Inflammation, Neovascularization, Pathologic, Pain Measurement, Platelet-Derived Growth Factor, Sensitivity and Specificity, Biomarkers, Interleukin-8, CA-125 Antigen, Vascular Endothelial Growth Factor A, Interleukin-1beta, Real-Time Polymerase Chain Reaction
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