The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Induction of a bystander mutagenic effect of alpha particles in mammalian cells
Open accessAbstract
Ever since the discovery of X-rays was made by Röntgen more than a hundred years ago, it has always been accepted that the deleterious effects of ionizing radiation such as mutation and carcinogenesis are attributable mainly to direct damage to DNA. Although evidence based on microdosimetric estimation in support of a bystander effect appears to be consistent, direct proof of such extranuclear/extracellular effects are limited. Using a precision charged particle microbeam, we show here that irradiation of 20% of randomly selected A L cells with 20 alpha particles each results in a mutant fraction that is 3-fold higher than expected, assuming no bystander modulation effect. Furthermore, analysis by multiplex PCR shows that the types of mutants induced are significantly different from those of spontaneous origin. Pretreatment of cells with the radical scavenger DMSO had no effect on the mutagenic incidence. In contrast, cells pretreated with a 40 μM dose of lindane, which inhibits cell–cell communication, significantly decreased the mutant yield. The doses of DMSO and lindane used in these experiments are nontoxic and nonmutagenic. We further examined the mutagenic yield when 5–10% of randomly selected cells were irradiated with 20 alpha particles each. Results showed, likewise, a higher mutant yield than expected assuming no bystander effects. Our studies provide clear evidence that irradiated cells can induce a bystander mutagenic response in neighboring cells not directly traversed by alpha particles and that cell–cell communication process play a critical role in mediating the bystander phenomenon.
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Cites
A simple salting out procedure for extracting DNA from human nucleated cells
Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.3.1215 · Cited by 20,330
Induction of sister chromatid exchanges by extremely low doses of alpha-particles.
Nagasawa, H., & Little, J. B. (1992) · PubMed · Cited by 995
Pelletier, J., Bruening, W., Kashtan, C. E., Mauer, S. M., Manivel, J. C., Striegel, J. E., Houghton, D. C., Junien, C., Habib, R., & Fouser, L. (1991) · Cell · doi:10.1016/0092-8674(91)90194-4 · Cited by 954
Lung Cancer Risk From Residential Radon: Meta-analysis of Eight Epidemiologic Studies
Lubin, J. H., & Boice, J. D. (1997) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/89.1.49 · Cited by 541
Azzam, E. I., de Toledo, S. M., Gooding, T., & Little, J. B. (1998) · Radiation Research · doi:10.2307/3579865 · Cited by 521
Narayanan, P. K., Goodwin, E. H., & Lehnert, B. E. (1997) · PubMed · Cited by 461
Targeted cytoplasmic irradiation with alpha particles induces mutations in mammalian cells
Wu, L.-J., Randers-Pehrson, G., Xu, A., Waldren, C. A., Geard, C. R., Yu, Z., & Hei, T. K. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.9.4959 · Cited by 444
Self-citation23 Genetic instability induced by the tumor microenvironment
Glazer, P. M., Reynolds, T. Y., & Rockwell, S. (1997) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/s0360-3016(97)80581-0 · Cited by 427
Mothersill, C., & Seymour, C. B. (1998) · Radiation Research · doi:10.2307/3579958 · Cited by 427
Mutagenicity of arsenic in mammalian cells: Role of reactive oxygen species
Hei, T. K., Liu, S. X., & Waldren, C. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.14.8103 · Cited by 422
Self-citation
Cited by
Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses
Rothkamm, K., & Löbrich, M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0830918100 · Cited by 1,645
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Sgouros, G., Roeske, J. C., McDevitt, M. R., Palm, S., Allen, B. J., Fisher, D. R., Brill, A. B., Song, H., Howell, R. W., & Akabani, G. (2010) · Journal of Nuclear Medicine · doi:10.2967/jnumed.108.058651 · Cited by 552
Radiation-Induced Bystander Effects: Past History and Future Directions
Mothersill, C., & Seymour, C. (2001) · Radiation Research · doi:10.1667/0033-7587(2001)155[0759:ribeph]2.0.co;2 · Cited by 507
DNA damage foci: Meaning and significance
Rothkamm, K., Barnard, S., Moquet, J., Ellender, M., Rana, Z., & Burdak‐Rothkamm, S. (2015) · Environmental and Molecular Mutagenesis · doi:10.1002/em.21944 · Cited by 335
Metadata
- Authors
- Zhou, H., Randers-Pehrson, G., Waldren, C. A., Vannais, D., Hall, E. J., & Hei, T. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-02-11
- Volume
- 97
- Issue
- 5
- Pages
- 2099-2104
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2132305988
- Cited by
- 477
- References
- 33
- FWCI
- 24.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Zhou, H., Randers-Pehrson, G., Waldren, C. A., Vannais, D., Hall, E. J., & Hei, T. K. (2000). Induction of a bystander mutagenic effect of alpha particles in mammalian cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.030420797
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