Cited by 477Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity

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Kawasaki, Hiroshi, Suemori, Hirofumi, Mizuseki, Kenji, Watanabe, Kiichi, Urano, Fumi, Ichinose, Hiroshi, Haruta, Masatoshi, Takahashi, Masayo +4 more·2002·Proceedings of the National Academy of Sciences

Abstract

We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH(+)) dopaminergic neurons, from mouse embryonic stem cells. We report here that SDIA induces efficient neural differentiation also in primate embryonic stem cells. Induced neurons contain TH(+) neurons at a frequency of 35% and produce a significant amount of dopamine. Interestingly, differentiation of TH(+) neurons from undifferentiated embryonic cells occurs much faster in vitro (10 days) than it does in the embryo (approximately 5 weeks). In addition, 8% of the colonies contain large patches of Pax6(+)-pigmented epithelium of the retina. The SDIA method provides an unlimited source of primate cells for the study of pathogenesis, drug development, and transplantation in degenerative diseases such as Parkinson's disease and retinitis pigmentosa.

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Crossref · 10.1073/pnas.032662199

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  • ESCs and iPSCs can spontaneously differentiate along the neural lineage and then to RPE cells, which are readily identified by pigmentation and cobblestone appearance.

    “ESCs and iPSCs can spontaneously differentiate along the neural lineage, and then to RPE cells, which are readily identified by virtue of pigmentation and cobblestone appearance (Kawasaki et al., 2002; Buchholz et al., 2009) (Fig. 4).”

    From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Kawasaki et al., 2002

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Authors
Kawasaki, H., Suemori, H., Mizuseki, K., Watanabe, K., Urano, F., Ichinose, H., Haruta, M., Takahashi, M., Yoshikawa, K., Nishikawa, S.-I., Nakatsuji, N., & Sasai, Y.
Affiliations
Fujita Health University (JP)
Venue
Proceedings of the National Academy of Sciences
Published
2002-01-29
Volume
99
Issue
3
Pages
1580-1585
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2161264600
Cited by
477
References
41
Citations by year
2026: 1 · 2025: 4 · 2024: 6 · 2023: 10 · 2022: 10 · 2021: 11 · 2020: 12 · 2019: 8 · 2018: 10 · 2017: 20 · 2016: 7 · 2015: 13 · 2014: 17 · 2013: 20 · 2012: 26
FWCI
13.36
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (submittedVersion)
  • Tokyo Tech Research Repository (Tokyo Institute of Technology) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
primate embryonic stem cells, dopaminergic neurons, tyrosine hydroxylase, neuronal differentiation, retinal pigment epithelium, Pax6, dopamine synthesis, Parkinson's disease, retinitis pigmentosa, cell transplantation
MeSH terms
Animals, Cell Differentiation, Cells, Cultured, Dopamine, Epithelial Cells, Macaca fascicularis, Neurons, Primates, Stem Cells, Time Factors, Transplantation, Heterologous, Tyrosine 3-Monooxygenase, Biomarkers, Mice, SCID, Stromal Cells, Cell Transplantation, Neural Cell Adhesion Molecules, Reverse Transcriptase Polymerase Chain Reaction, Mice

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