The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Nanometer-localized multiple single-molecule fluorescence microscopy
Open accessAbstract
Fitting the image of a single molecule to the point spread function of an optical system greatly improves the precision with which single molecules can be located. Centroid localization with nanometer precision has been achieved when a sufficient number of photons are collected. However, if multiple single molecules reside within a diffraction-limited spot, this localization approach does not work. This paper demonstrates nanometer-localized multiple single-molecule (NALMS) fluorescence microscopy by using both centroid localization and photobleaching of the single fluorophores. Short duplex DNA strands are used as nanoscale “rulers” to validate the NALMS microscopy approach. Nanometer accuracy is demonstrated for two to five single molecules within a diffraction-limited area. NALMS microscopy will greatly facilitate single-molecule study of biological systems because it covers the gap between fluorescence resonance energy transfer-based (<10 nm) and diffraction-limited microscopy (>100 nm) measurements of the distance between two fluorophores. Application of NALMS microscopy to DNA mapping with <10-nm (i.e., 30-base) resolution is demonstrated.
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Cites
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Cited by
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Super-resolution fluorescence microscopy
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Switchable Fluorophores for Single-Molecule Localization Microscopy
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Metadata
- Authors
- Qu, X., Wu, D., Mets, L., & Scherer, N. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-07-26
- Volume
- 101
- Issue
- 31
- Pages
- 11298-11303
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2148283489
- Cited by
- 304
- References
- 24
- FWCI
- 9.48
- Type
- journal article
- Language
- en
- Open access
- green
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