The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Biochemical evidence for the association of fragile X mental retardation protein with brain polyribosomal ribonucleoparticles
Open accessAbstract
Fragile X syndrome is caused by the absence of the fragile X mental retardation protein (FMRP). This RNA-binding protein is widely expressed in human and mouse tissues, and it is particularly abundant in the brain because of its high expression in neurons, where it localizes in the cell body and in granules throughout dendrites. Although FMRP is thought to regulate trafficking of repressed mRNA complexes and to influence local protein synthesis in synapses, it is not known whether it has additional functions in the control of translation in the cell body. Here, we have used recently developed approaches to investigate whether FMRP is associated with the translation apparatus. We demonstrate that, in the brain, FMRP is present in actively translating polyribosomes, and we show that this association is acutely sensitive to the type of detergent required to release polyribosomes from membranous structures. In addition, proteomic analyses of purified brain polyribosomes reveal the presence of several RNA-binding proteins that, similarly to FMRP, have been previously localized in neuronal granules. Our findings highlight the complex roles of FMRP both in actively translating polyribosomes and in repressed trafficking ribonucleoparticle granules.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“FMRP is a polyribosome-associated neuronal RNA-binding protein, suggesting that it plays a key role in regulating neuronal translation, but there has been little consensus regarding either its RNA targets or mechanism of action.”
From FMRP Stalls Ribosomal Translocation on mRNAs Linked to Synaptic Function and Autism (2011) · cites this paper as Khandjian et al., 2004
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation
Gingras, A., Raught, B., & Sonenberg, N. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.913 · Cited by 2,103
Messenger-RNA-binding proteins and the messages they carry
Dreyfuss, G., Kim, V. N., & Kataoka, N. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm760 · Cited by 1,457
Brown, V., Jin, P., Ceman, S., Darnell, J. C., O'Donnell, W. T., Tenenbaum, S. A., Jin, X., Feng, Y., Wilkinson, K. D., Keene, J. D., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00568-2 · Cited by 1,109
Abnormal dendritic spines in fragile X knockout mice: Maturation and pruning deficits
Comery, T. A., Harris, J. B., Willems, P. J., Oostra, B. A., Irwin, S. A., Weiler, I. J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5401 · Cited by 1,046
Devys, D., Lutz, Y., Rouyer, N., Bellocq, J., & Mandel, J. (1993) · Nature Genetics · doi:10.1038/ng0893-335 · Cited by 781
Ribosomal Aggregate Engaged in Protein Synthesis: Characterization of the Ergosome
Wettstein, F. O., Staehelin, T., & Noll, H. (1963) · Nature · doi:10.1038/197430a0 · Cited by 745
Gene-Specific Regulation by General Translation Factors
Dever, T. E. (2002) · Cell · doi:10.1016/s0092-8674(02)00642-6 · Cited by 730
Zalfa, F., Giorgi, M., Primerano, B., Moro, A., Di Penta, A., Reis, S., Oostra, B., & Bagni, C. (2003) · Cell · doi:10.1016/s0092-8674(03)00079-5 · Cited by 676
Weiler, I. J., Irwin, S. A., Klintsova, A. Y., Spencer, C. M., Brazelton, A. D., Miyashiro, K., Comery, T. A., Patel, B., Eberwine, J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5395 · Cited by 644
Jin, P., Zarnescu, D. C., Ceman, S., Nakamoto, M., Mowrey, J., Jongens, T. A., Nelson, D. L., Moses, K., & Warren, S. T. (2004) · Nature Neuroscience · doi:10.1038/nn1174 · Cited by 604
Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
Kim, J., Krichevsky, A. M., Grad, Y. H., Hayes, G. D., Kosik, K. S., Church, G. M., & Ruvkun, G. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2333854100 · Cited by 586
Evidence that fragile X mental retardation protein is a negative regulator of translation
Laggerbauer, B. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.4.329 · Cited by 571
Abnormal Development of Dendritic Spines in FMR1 Knock-Out Mice
Nimchinsky, E. A., Oberlander, A. M., & Svoboda, K. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-14-05139.2001 · Cited by 560
Krichevsky, A. M., & Kosik, K. S. (2001) · Neuron · doi:10.1016/s0896-6273(01)00508-6 · Cited by 536
Feng, Y., Gutekunst, C.-A., Eberhart, D. E., Yi, H., Warren, S. T., & Hersch, S. M. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-05-01539.1997 · Cited by 532
Analysis of neocortex in three males with the Fragile X syndrome
Hinton, V. J., Brown, W. T., Wisniewski, K., & Rudelli, R. D. (1991) · American Journal of Medical Genetics · doi:10.1002/ajmg.1320410306 · Cited by 527
Feng, Y., Absher, D., Eberhart, D. E., Brown, V., Malter, H. E., & Warren, S. T. (1997) · Molecular Cell · doi:10.1016/s1097-2765(00)80012-x · Cited by 511
The pleiotropic functions of the Y‐box‐binding protein, YB‐1
Kohno, K., Izumi, H., Uchiumi, T., Ashizuka, M., & Kuwano, M. (2003) · BioEssays · doi:10.1002/bies.10300 · Cited by 502
RNA cargoes associating with FMRP reveal deficits in cellular functioning in Fmr1 null mice
Miyashiro, K. Y., Beckel-Mitchener, A., Purk, T. P., Becker, K. G., Barret, T., Liu, L., Carbonetto, S., Weiler, I. J., Greenough, W. T., & Eberwine, J. (2003) · Neuron · doi:10.1016/s0896-6273(03)00034-5 · Cited by 495
A Decade of Molecular Studies of Fragile X Syndrome
O'Donnell, W. T., & Warren, S. T. (2002) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.25.112701.142909 · Cited by 487
The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif
Schaeffer, C., Bardoni, B., Mandel, J., Ehresmann, B., Ehresmann, C., & Moine, H. (2001) · The EMBO Journal · doi:10.1093/emboj/20.17.4803 · Cited by 471
Self-citationAdam, S. A., Nakagawa, T. Y., Swanson, M. S., Woodruff, T. K., & Dreyfuss, G. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.8.2932 · Cited by 465
Studies on free and membrane-bound ribosomes in rat liver
Blobel, G., & Potter, V. R. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90297-5 · Cited by 456
The fragile X mental retardation protein inhibits translation via interacting with mRNA
Li, Z. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.11.2276 · Cited by 448
mRNA localization: message on the move
Jansen, R. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35067016 · Cited by 414
Antar, L. N., Afroz, R., Dictenberg, J. B., Carroll, R. C., & Bassell, G. J. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.0099-04.2004 · Cited by 411
Eberhart, D. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.8.1083 · Cited by 390
Axonal Protein Synthesis Provides a Mechanism for Localized Regulation at an Intermediate Target
Brittis, P. A., Lü, Q., & Flanagan, J. G. (2002) · Cell · doi:10.1016/s0092-8674(02)00813-9 · Cited by 381
Compartmentalized Synthesis and Degradation of Proteins in Neurons
Steward, O., & Schuman, E. M. (2003) · Neuron · doi:10.1016/s0896-6273(03)00635-4 · Cited by 377
Mazrouï, R. (2002) · Human Molecular Genetics · doi:10.1093/hmg/11.24.3007 · Cited by 369
Synaptic regulation of protein synthesis and the fragile X protein
Greenough, W. T., Klintsova, A. Y., Irwin, S. A., Galvez, R., Bates, K. E., & Weiler, I. J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.141145998 · Cited by 317
Local protein synthesis and its role in synapse-specific plasticity
Martin, K. C., Barad, M., & Kandel, E. R. (2000) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(00)00128-8 · Cited by 262
The fragile X mental retardation protein is associated with ribosomes
Khandjian, E. W., Corbin, F., Woerly, S., & Rousseau, F. (1996) · Nature Genetics · doi:10.1038/ng0196-91 · Cited by 251
Corbin, F. (1997) · Human Molecular Genetics · doi:10.1093/hmg/6.9.1465 · Cited by 239
mRNA and cytoskeletal filaments
Bassell, G. J. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80159-7 · Cited by 218
Ultrabithorax function in butterfly wings and the evolution of insect wing patterns
Weatherbee, S. D., Nijhout, H. F., Grunert, L. W., Halder, G. A., Galant, R., Selegue, J. E., & Carroll, S. B. (1999) · Current Biology · doi:10.1016/s0960-9822(99)80064-5 · Cited by 217
Molecular Insights into mRNA Transport and Local Translation in the Mammalian Nervous System
Kiebler, M. A., & DesGroseillers, L. (2000) · Neuron · doi:10.1016/s0896-6273(00)80868-5 · Cited by 202
Preparation of mammalian polyribosomes with the detergent Nonidet P-40
Borun, T. W., Scharff, M. D., & Robbins, E. S. (1967) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(67)90715-0 · Cited by 199
Valášek, L., Mathew, A., Shin, B., Nielsen, K. H., Szamecz, B., & Hinnebusch, A. (2003) · Genes & Development · doi:10.1101/gad.1065403 · Cited by 164
Jansen, R. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.3.455 · Cited by 150
Transport of Fragile X Mental Retardation Protein via Granules in Neurites of PC12 Cells
de Diego‐Otero, Y., Severijnen, L.-A. W. F. M., van Cappellen, G. W. A., Schrier, M., Oostra, B. A., & Willemsen, R. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.23.8332-8341.2002 · Cited by 149
Khandjian, E. W., Anny, F., Thibodeau, A., Tremblay, S., Côté, F., Devys, D., Mandel, J.-L., & Rousseau, F. (1995) · Human Molecular Genetics · doi:10.1093/hmg/4.5.783 · Cited by 145
Self-citationBardoni, B., & Mandel, J. (2002) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(02)00300-3 · Cited by 129
Self-citationDuchaîne, T. F., Hemraj, I., Furic, L., Deitinghoff, A., Kiebler, M. A., & DesGroseillers, L. (2002) · Journal of Cell Science · doi:10.1242/jcs.115.16.3285 · Cited by 128
Antar, L. N., & Bassell, G. J. (2003) · Neuron · doi:10.1016/s0896-6273(03)00090-4 · Cited by 116
Novel isoforms of the fragile X related protein FXR1P are expressed during myogenesis
Khandjian, E. W. (1998) · Human Molecular Genetics · doi:10.1093/hmg/7.13.2121 · Cited by 114
Self-citationDavydova, E. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.14.2911 · Cited by 100
Dendritic localization of mRNAs
Kuhl, D., & Skehel, P. (1998) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(98)80087-1 · Cited by 97
Mazrouï, R. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddg335 · Cited by 82
Bardoni, B. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddg181 · Cited by 79
Self-citationThe fragile X mental retardation protein has nucleic acid chaperone properties
Gabus, C. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh535 · Cited by 74
Selective translation of mRNAs at synapses
Richter, J. D. (2002) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(02)00318-5 · Cited by 68
Bardoni, B. (2003) · Experimental Cell Research · doi:10.1016/s0014-4827(03)00222-2 · Cited by 66
Self-citationCharacterization of Protein Bound to Rapidly‐Labelled RNA in Polyribosomes from Rat Liver
Olsnes, S. (1970) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1970.tb01029.x · Cited by 64
Particules ribonucléoprotéiques 40s des noyaux de foie de rat
MOULÉ, Y., & Chauveau, J. (1968) · Journal of Molecular Biology · doi:10.1016/0022-2836(68)90203-9 · Cited by 61
Gene Expression Profiles in a Transgenic Animal Model of Fragile X Syndrome
D’Agata, V., Warren, S. T., Zhao, W., Torre, E. R., Alkon, D. L., & Cavallaro, S. (2002) · Neurobiology of Disease · doi:10.1006/nbdi.2002.0506 · Cited by 54
Protein synthesis in axons and its possible functions
Campenot, R. B., & Eng, H. (2000) · Journal of Neurocytology · doi:10.1023/a:1010939307434 · Cited by 50
Olsnes, S. (1970) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(70)90016-x · Cited by 47
Ramsey, J. C., & Steele, W. J. (1977) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1977.tb10422.x · Cited by 44
Grange, J., Boyer, V., Fabian‐Fine, R., Fredj, N. B., Sadoul, R., & Goldberg, Y. (2004) · Journal of Neuroscience Research · doi:10.1002/jnr.20003 · Cited by 24
Cataract extraction in high myopic eyes.
Ku, W., Chuang, L., & Lai, C. (2002) · PubMed · Cited by 24
Darnell, R. B. (2002) · Cell · doi:10.1016/s0092-8674(02)00937-6 · Cited by 19
Irwin (2001) · doi:10.1002/1096-8628(20010115)98:2
Flagged first, then by citations
Research works printed but not linked: 1
- 10.1128/mcb.6.8.2932-2943.1986 · DOI
Cited by
Sorted by citations
FMRP Stalls Ribosomal Translocation on mRNAs Linked to Synaptic Function and Autism
Darnell, J. C., Van Driesche, S. J., Zhang, C., Hung, K. Y. S., Mele, A., Fraser, C. E., Stone, E. F., Chen, C., Fak, J. J., Chi, S. W., et al. (2011) · Cell · doi:10.1016/j.cell.2011.06.013 · Cited by 2,072
Stress granules: the Tao of RNA triage
Anderson, P., & Kedersha, N. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.12.003 · Cited by 1,161
Fragile X Syndrome: Loss of Local mRNA Regulation Alters Synaptic Development and Function
Bassell, G. J., & Warren, S. T. (2008) · Neuron · doi:10.1016/j.neuron.2008.10.004 · Cited by 1,051
Dendritic mRNA: transport, translation and function
Bramham, C. R., & Wells, D. G. (2007) · Nature reviews. Neuroscience · doi:10.1038/nrn2150 · Cited by 596
Molecular Mechanisms of Fragile X Syndrome: A Twenty-Year Perspective
Santoro, M. R., Bray, S. M., & Warren, S. T. (2012) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathol-011811-132457 · Cited by 528
Metadata
- Authors
- Khandjian, E. W., Huot, M.-E., Tremblay, S., Davidovic, L., Mazroui, R., & Bardoni, B.
- Affiliations
- Unité de Recherche en Génétique Humaine et Moléculaire, Centre de Recherche Hôpital Saint-François d'Assise, Centre Hospitalier Universitaire de Québec, Québec, QC, Canada G1L 3L5; Département de Biologie Médicale, Faculté de Médecine, Université Laval, Québec, QC, Canada G1K 7P4; and Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur, BP 10142, 67404...
- Centre National de la Recherche Scientifique (FR)
- ORCID
- Bardoni, Barbara
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-08-25
- Volume
- 101
- Issue
- 36
- Pages
- 13357-13362
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC516571
- OpenAlex
- W2049676741
- Cited by
- 178
- References
- 63
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 7 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 5 · 2014: 10 · 2013: 12 · 2012: 8
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- FMRP, polysomes, ribonucleoprotein particles, fragile X syndrome, RNA-binding proteins, translational regulation, mRNA trafficking, brain, detergent sensitivity
- MeSH terms
- Animals, Brain, Fragile X Syndrome, Intellectual Disability, Nerve Tissue Proteins, Polyribosomes, Ribonucleoproteins, Protein Biosynthesis, RNA-Binding Proteins, Mice, Fragile X Messenger Ribonucleoprotein 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
