The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei
Open accessAbstract
Bacterial symbionts have long been suspected to be the true producers of many drug candidates isolated from marine invertebrates. Sponges, the most important marine source of biologically active natural products, have been frequently hypothesized to contain compounds of bacterial origin. This symbiont hypothesis, however, remained unproven because of a general inability to cultivate the suspected producers. However, we have recently identified an uncultured Pseudomonas sp. symbiont as the most likely producer of the defensive antitumor polyketide pederin in Paederus fuscipes beetles by cloning the putative biosynthesis genes. Here we report closely related genes isolated from the highly complex metagenome of the marine sponge Theonella swinhoei , which is the source of the onnamides and theopederins, a group of polyketides that structurally resemble pederin. Sequence features of the isolated genes clearly indicate that it belongs to a prokaryotic genome and should be responsible for the biosynthesis of almost the entire portion of the polyketide structure that is correlated with antitumor activity. Besides providing further proof for the role of the related beetle symbiont-derived genes, these findings raise intriguing ecological and evolutionary questions and have important general implications for the sustainable production of otherwise inaccessible marine drugs by using biotechnological strategies.
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Cites
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Molecular Evidence for a Uniform Microbial Community in Sponges from Different Oceans
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Piel, J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.222481399 · Cited by 609
Self-citationMetabolites from symbiotic bacteria
Piel, J. (2009) · Nat. Prod. Rep. · doi:10.1039/b703499g · Cited by 577
Self-citationEdwards, D. J., Marquez, B. L., Nogle, L. M., McPhail, K., Goeger, D. E., Roberts, M. A., & Gerwick, W. H. (2004) · Chemistry & Biology · doi:10.1016/j.chembiol.2004.03.030 · Cited by 506
Genome fragment of Wolbachia endosymbiont transferred to X chromosome of host insect
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Piel, J. (2004) · Natural Product Reports · doi:10.1039/b310175b · Cited by 311
Self-citationDiscovery of the Novel Candidate Phylum “ Poribacteria ” in Marine Sponges
Fieseler, L., Horn, M., Wagner, M., & Hentschel, U. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.6.3724-3732.2004 · Cited by 301
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Cited by
Natural products as sources of new drugs over the last 25 years
Newman, D. J., & Cragg, G. M. (2007) · Journal of Natural Products · doi:10.1021/np068054v · Cited by 5,517
Faulkner, D. J. (1990) · Natural Product Reports · doi:10.1039/np9900700269 · Cited by 4,866
Pathogenic fungus harbours endosymbiotic bacteria for toxin production
Partida-Martinez, L. P., & Hertweck, C. (2005) · Nature · doi:10.1038/nature03997 · Cited by 677
Genomic insights into the marine sponge microbiome
Hentschel, U., Piel, J., Degnan, S. M., & Taylor, M. W. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2839 · Cited by 658
Self-citationSchmidt, E. W., Nelson, J. T., Rasko, D. A., Sudek, S., Eisen, J. A., Haygood, M. G., & Ravel, J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501424102 · Cited by 567
Metadata
- Authors
- Piel, J., Hui, D., Wen, G., Butzke, D., Platzer, M., Fusetani, N., & Matsunaga, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-11
- Volume
- 101
- Issue
- 46
- Pages
- 16222-16227
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2011757239
- Cited by
- 543
- References
- 28
- FWCI
- 22.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Piel, J., Hui, D., Wen, G., Butzke, D., Platzer, M., Fusetani, N., & Matsunaga, S. (2004). Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0405976101
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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