Cited by 569Microbial Natural Products and BiosynthesisMarine Sponges and Natural ProductsSynthesis and Biological Activity

Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni , the cyanobacterial symbiont of Lissoclinum patella

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Schmidt, Eric W., Nelson, James T., Rasko, David A., Sudek, Sebastian, Eisen, Jonathan A., Haygood, Margo G., Ravel, Jacques·2005·Proceedings of the National Academy of Sciences

Abstract

Prochloron spp. are obligate cyanobacterial symbionts of many didemnid family ascidians. It has been proposed that the cyclic peptides of the patellamide class found in didemnid extracts are synthesized by Prochloron spp., but studies in which host and symbiont cells are separated and chemically analyzed to identify the biosynthetic source have yielded inconclusive results. As part of the Prochloron didemni sequencing project, we identified patellamide biosynthetic genes and confirmed their function by heterologous expression of the whole pathway in Escherichia coli . The primary sequence of patellamides A and C is encoded on a single ORF that resembles a precursor peptide. We propose that this prepatellamide is heterocyclized to form thiazole and oxazoline rings, and the peptide is cleaved to yield the two cyclic patellamides, A and C. This work represents the full sequencing and functional expression of a marine natural-product pathway from an obligate symbiont. In addition, a related cluster was identified in Trichodesmium erythraeum IMS101, an important bloom-forming cyanobacterium.

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  1. Piel, J., Hui, D., Wen, G., Butzke, D., Platzer, M., Fusetani, N. & Matsunaga, S. (2004) Proc. Natl. Acad. Sci. USA 101, 16222-16227.
  2. Withers, N. W., Alberte, R. S., Lewin, R. A., Thornber, J. P., Britton, G. & Goodwin, T. W. (1978) Proc. Natl. Acad. Sci. USA 75, 2301-2305.
  3. Yorgey, P., Lee, J., Kordel, J., Vivas, E., Warner, P., Jebaratnam, D. & Kolter, R. (1994) Proc. Natl. Acad. Sci. USA 91, 4519-4523.

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Authors
Schmidt, E. W., Nelson, J. T., Rasko, D. A., Sudek, S., Eisen, J. A., Haygood, M. G., & Ravel, J.
Affiliations
Scripps Institution of Oceanography (US)
Venue
Proceedings of the National Academy of Sciences
Published
2005-05-09
Volume
102
Issue
20
Pages
7315-7320
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2162481919
Cited by
567
References
53
Citations by year
2026: 12 · 2025: 14 · 2024: 14 · 2023: 12 · 2022: 27 · 2021: 22 · 2020: 22 · 2019: 28 · 2018: 26 · 2017: 33 · 2016: 26 · 2015: 27 · 2014: 38 · 2013: 32 · 2012: 41
FWCI
18.29
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion, other-oa)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, other-oa)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
National Science Foundation (0412226)
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Marine Sponges and Natural Products
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Synthesis and Biological Activity
Keywords
cyanobacterial symbionts, Lissoclinum patella, heterocyclization, biosynthetic gene clusters, Trichodesmium erythraeum, marine natural products, heterologous expression
MeSH terms
Cyanobacteria, Amino Acid Sequence, Animals, Bacteriocins, Chromatography, High Pressure Liquid, Escherichia coli, Molecular Sequence Data, Peptides, Cyclic, Symbiosis, Urochordata, Open Reading Frames, Sequence Analysis, DNA, DNA Primers, Palau, Prochloron

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