The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo
Open accessAbstract
Silencing of gene expression by small interfering RNAs (siRNAs) is rapidly becoming a powerful tool for genetic analysis and represents a potential strategy for therapeutic product development. However, there are no reports of systemic delivery for siRNAs toward treatment of bone-metastatic cancer. Accordingly, we report here that i.v. injection of GL3 luciferase siRNA complexed with atelocollagen showed effective reduction of luciferase expression from bone-metastatic prostate tumor cells developed in mouse thorax, jaws, and/or legs. We also show that the siRNA/atelocollagen complex can be efficiently delivered to tumors 24 h after injection and can exist intact at least for 3 days. Furthermore, atelocollagen-mediated systemic administration of siRNAs such as enhancer of zeste homolog 2 and phosphoinositide 3′-hydroxykinase p110-α-subunit, which were selected as candidate targets for inhibition of bone metastasis, resulted in an efficient inhibition of metastatic tumor growth in bone tissues. In addition, upregulation of serum IL-12 and IFN-α levels was not associated with the in vivo administration of the siRNA/atelocollagen complex. Thus, for treatment of bone metastasis of prostate cancer, an atelocollagen-mediated systemic delivery method could be a reliable and safe approach to the achievement of maximal function of siRNA.
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1 retracted
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Cited by
Knocking down barriers: advances in siRNA delivery
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Lipid-like materials for low-dose, in vivo gene silencing
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In vivo imaging of siRNA delivery and silencing in tumors
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Progress towards in Vivo Use of siRNAs
Behlke, M. A. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.01.001 · Cited by 677
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Metadata
- Authors
- Takeshita, F., Minakuchi, Y., Nagahara, S., Honma, K., Sasaki, H., Hirai, K., Teratani, T., Namatame, N., Yamamoto, Y., Hanai, K., Kato, T., Sano, A., & Ochiya, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-08-09
- Volume
- 102
- Issue
- 34
- Pages
- 12177-12182
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2112962172
- Cited by
- 355
- References
- 33
- FWCI
- 10.37
- Type
- journal article
- Language
- en
- Open access
- green
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- Takeshita, F., Minakuchi, Y., Nagahara, S., Honma, K., Sasaki, H., Hirai, K., Teratani, T., Namatame, N., Yamamoto, Y., Hanai, K., Kato, T., Sano, A., & Ochiya, T. (2005). Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0501753102
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