In vivo imaging of siRNA delivery and silencing in tumors
Abstract
With the increased potential of RNA interference (RNAi) as a therapeutic strategy, new noninvasive methods for detection of siRNA delivery and silencing are urgently needed. Here we describe the development of dual-purpose probes for in vivo transfer of siRNA and the simultaneous imaging of its accumulation in tumors by high-resolution magnetic resonance imaging (MRI) and near-infrared in vivo optical imaging (NIRF). These probes consisted of magnetic nanoparticles labeled with a near-infrared dye and covalently linked to siRNA molecules specific for model or therapeutic targets. Additionally, these nanoparticles were modified with a membrane translocation peptide for intracellular delivery. We show the feasibility of in vivo tracking of tumor uptake of these probes by MRI and optical imaging in two separate tumor models. We also used proof-of-principle optical imaging to corroborate the efficiency of the silencing process. These studies represent the first step toward the advancement of siRNA delivery and imaging strategies, essential for cancer therapeutic product development and optimization.
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Medarova et al. achieved substantial silencing of GFP and survivin in tumors using a SPION-based theranostic nanoplatform for siRNA delivery, comparable or superior to previous studies.
“They achieved substantial silencing (of both GFP and survivin) in tumors, which was comparable or superior to previous published studies. This study thereby provided pioneering proof-of-concept for a SPION-based theranostic nanoplatform for siRNA delivery [239].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [239]
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- Authors
- Medarova, Z., Pham, W., Farrar, C., Petkova, V., & Moore, A.
- Affiliations
- Harvard University (US)
- Beth Israel Deaconess Medical Center (US)
- Corresponding author
- Moore, Anna
- Venue
- Nature Medicine
- Published
- 2007-02-25
- Volume
- 13
- Issue
- 3
- Pages
- 372-377
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2138436760
- Cited by
- 697
- References
- 35
- Citations by year
- 2026: 5 · 2025: 12 · 2024: 15 · 2023: 13 · 2022: 15 · 2021: 20 · 2020: 20 · 2019: 19 · 2018: 24 · 2017: 34 · 2016: 41 · 2015: 36 · 2014: 53 · 2013: 65 · 2012: 63
- FWCI
- 25.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (K25AG029415)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- siRNA delivery, RNA interference, in vivo imaging, magnetic nanoparticles, tumor targeting, intracellular delivery, gene silencing, tumor uptake, cancer therapeutics
- MeSH terms
- Adenocarcinoma, Animals, Humans, Magnetic Resonance Imaging, Colorectal Neoplasms, Molecular Probes, Spectroscopy, Near-Infrared, Gene Silencing, RNA, Small Interfering, Cell Line, Tumor, Mice
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