Cited by 171Mitochondrial Function and PathologyAnimal Genetics and ReproductionCRISPR and Genetic Engineering

Gene therapy for progeny of mito-mice carrying pathogenic mtDNA by nuclear transplantation

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Sato, Akitsugu, Kono, Tomohiro, Nakada, Kazuto, Ishikawa, Kaori, Inoue, Shin-Ichi, Yonekawa, Hiromichi, Hayashi, Jun-Ichi·2005·Proceedings of the National Academy of Sciences

Abstract

Pathogenic mutations in mtDNAs have been shown to be responsible for expression of respiration defects and resultant expression of mitochondrial diseases. This study directly addressed the issue of gene therapy of mitochondrial diseases by using nuclear transplantation of zygotes of transmitochondria mice (mito-mice). Mito-mice expressed respiration defects and mitochondrial diseases due to accumulation of mtDNA carrying a large-scale deletion (DeltamtDNA). Second polar bodies were used as biopsy samples for diagnosis of mtDNA genotypes of mito-mouse zygotes. Nuclear transplantation was carried out from mito-mouse zygotes to enucleated normal zygotes and was shown to rescue all of the F(0) progeny from expression of respiration defects throughout their lives. This procedure should be applicable to patients with mitochondrial diseases for preventing their children from developing the diseases.

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Crossref · 10.1073/pnas.0506197102

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  • Previous studies in heteroplasmic mice have suggested the feasibility of pronuclear transfer.

    “Previous studies in heteroplasmic mice have suggested the feasibility of this approach [253].”

    From Mitochondrial DNA mutations and human disease (2010) · cites this paper as [253]

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Authors
Sato, A., Kono, T., Nakada, K., Ishikawa, K., Inoue, S.-I., Yonekawa, H., & Hayashi, J.-I.
Affiliations
  • Tokyo University of Agriculture (JP)
  • Graduate School of Life and Environmental Sciences, Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki 305-8572, Japan; Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan; Department of BioScience, Tokyo University of Agriculture, Tokyo 156-8502, Japan; and Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan
Venue
Proceedings of the National Academy of Sciences
Published
2005-11-07
Volume
102
Issue
46
Pages
16765-16770
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2063371306
Cited by
171
References
22
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 8 · 2022: 3 · 2021: 9 · 2020: 8 · 2019: 6 · 2018: 8 · 2017: 10 · 2016: 12 · 2015: 6 · 2014: 13 · 2013: 10 · 2012: 4
FWCI
3.53
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords
gene therapy, mitochondrial disease, nuclear transplantation, mitochondrial DNA deletions, polar body biopsy, mitochondrial DNA
MeSH terms
Aging, Animals, DNA, Mitochondrial, Genotype, Growth, Mice, Inbred C57BL, Mice, Mutant Strains, Oxygen Consumption, Phenotype, Blotting, Southern, Genetic Therapy, Fetal Development, Mice, Nuclear Transfer Techniques

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