The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells
Abstract
Infections with hepatitis C virus (HCV) are marked by frequent viral persistence, chronic liver disease, and extraordinary viral genetic diversity. Although much has been learned about HCV since its discovery, progress has been slowed by a lack of permissive cell culture systems supporting its replication. Productive infections have been achieved recently with genotype 2a virus, but cirrhosis and liver cancer are typically associated with genotype 1 HCV, which is more prevalent and relatively resistant to IFN therapy. We describe production of infectious genotype 1a HCV in cells transfected with synthetic RNA derived from a prototype virus (H77-S). Viral proteins accumulated more slowly in H77-S transfected cells than in cells transfected with genotype 2a (JFH-1) RNA, but substantially more H77-S RNA was secreted into supernatant fluids. Most secreted RNA was noninfectious, banding in isopycnic gradients at a density of 1.04–1.07 gm∕cm 3 , but infectivity was associated with H77-S particles possessing a density of 1.13–1.14 gm∕cm 3 . The specific infectivity of H77-S particles (5.4 × 10 4 RNA copies per focus-forming unit) was significantly lower than JFH-1 virus (1.4 × 10 2 RNA copies per focus-forming unit). Infection with either virus was blocked by CD81 antibody. Sera from genotype 1a-infected individuals neutralized H77-S virus, but had little activity against genotype 2a virus, suggesting that these genotypes represent different serotypes. The ability of this genotype 1a virus to infect cultured cells will substantially benefit antiviral and vaccine discovery programs.
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Cites
The HCV Life Cycle: In vitro Tissue Culture Systems and Therapeutic Targets
Gerold, G., & Pietschmann, T. (2014) · Digestive Diseases · doi:10.1159/000360830 · Cited by 4,854
Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line
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Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
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Self-citationComplete Replication of Hepatitis C Virus in Cell Culture
Lindenbach, B. D., Evans, M. J., Syder, A. J., Wölk, B., Tellinghuisen, T. L., Liu, C. C., Maruyama, T., Hynes, R. O., Burton, D. R., McKeating, J. A., et al. (2005) · Science · doi:10.1126/science.1114016 · Cited by 2,238
Binding of Hepatitis C Virus to CD81
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Robust hepatitis C virus infection in vitro
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Self-citationConsensus Proposals for a Unified System of Nomenclature of Hepatitis C Virus Genotypes *
Simmonds, P., Bukh, J., Combet, C., Deléage, G., Enomoto, N., Feinstone, S., Halfon, P., Inchauspé, G., Kuiken, C., Maertens, G., et al. (2005) · Hepatology · doi:10.1002/hep.20819 · Cited by 1,516
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Highly Permissive Cell Lines for Subgenomic and Genomic Hepatitis C Virus RNA Replication
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Appel, N., Zayas, M., Miller, S., Krijnse-Locker, J., Schaller, T., Friebe, P., Kallis, S., Engel, U., & Bartenschlager, R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000035 · Cited by 466
Mitchell, J. K., Midkiff, B. R., Israelow, B., Evans, M. J., Lanford, R. E., Walker, C. M., Lemon, S. M., & McGivern, D. R. (2017) · mBio · doi:10.1128/mbio.00121-17 · Cited by 38
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Metadata
- Authors
- Yi, M., Villanueva, R. A., Thomas, D. L., Wakita, T., & Lemon, S. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-02-06
- Volume
- 103
- Issue
- 7
- Pages
- 2310-2315
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2119303435
- Cited by
- 357
- References
- 24
- FWCI
- 29.04
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells. https://app.cochat.ai/papers/10.1073/pnas.0510727103
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- Yi, M., Villanueva, R. A., Thomas, D. L., Wakita, T., & Lemon, S. M. (2006). Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0510727103
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