The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis
Abstract
The B3 domain protein LEAFY COTYLEDON2 (LEC2) is required for several aspects of embryogenesis, including the maturation phase, and is sufficient to induce somatic embryo development in vegetative cells. Here, we demonstrate that LEC2 directly controls a transcriptional program involved in the maturation phase of seed development. Induction of LEC2 activity in seedlings causes rapid accumulation of RNAs normally present primarily during the maturation phase. Several RNAs encode proteins with known roles in maturation processes, including seed-storage and lipid-body proteins. Clustering analyses identified other LEC2-induced RNAs not previously shown to be involved in the maturation phase. We show further that genes encoding these maturation RNAs all possess in their 5′ flanking regions RY motifs, DNA elements bound by other closely related B3 domain transcription factors. Our finding that recombinant LEC2 specifically binds RY motifs from the 5′ flanking regions of LEC2-induced genes provides strong evidence that these genes represent transcriptional targets of LEC2. Although these LEC2-induced RNAs accumulate primarily during the maturation phase, we show that a subset, including AGL15 and IAA30, accumulate in seeds containing zygotes. We discuss how identification of LEC2 target genes provides a potential link between the roles of LEC2 in the maturation phase and in the induction of somatic embryogenesis.
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LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development
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Cited by
Molecular networks regulating Arabidopsis seed maturation, after‐ripening, dormancy and germination
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Metadata
- Authors
- Braybrook, S. A., Stone, S. L., Park, S., Bui, A. Q., Le, B. H., Fischer, R. L., Goldberg, R. B., & Harada, J. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-02-21
- Volume
- 103
- Issue
- 9
- Pages
- 3468-3473
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2153677263
- Cited by
- 373
- References
- 50
- FWCI
- 26.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Braybrook, S. A., Stone, S. L., Park, S., Bui, A. Q., Le, B. H., Fischer, R. L., Goldberg, R. B., & Harada, J. J. (2006). Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0511331103
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