Cited by 279Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

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Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode

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Balaji, J., Ryan, T. A.·2007·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0707574105PMC PMC2154473

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Abstract

The nature of synaptic vesicle recycling at nerve terminals has been a subject of considerable debate for >35 years. Here, we report the use of an optical strategy that allows the exocytosis and retrieval of synaptic components to be tracked in real time at single-molecule sensitivity in living nerve terminals. This approach has allowed us to examine the recycling of synaptic vesicles in response to single action potentials. Our results show that, after exocytosis, individual synaptic vesicles are retrieved by a stochastic process with an exponential distribution of delay times, with a mean time of approximately 14 s. We propose that evidence for fast endocytosis, such as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic nature of a slower process.

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Authors: Balaji, J., & Ryan, T. A.
Affiliations: Weill Cornell Medicine (US)
ORCID: Balaji, J.
Ryan, T. A.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2007-12-18
Volume: 104
Issue: 51
Pages: 20576-20581
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0707574105
PMC: PMC2154473
OpenAlex: W2008643711
Cited by: 279
References: 24
Citations by year: 2026: 5 · 2025: 12 · 2024: 11 · 2023: 7 · 2022: 18 · 2021: 12 · 2020: 14 · 2019: 17 · 2018: 18 · 2017: 19 · 2016: 12 · 2015: 14 · 2014: 23 · 2013: 20 · 2012: 10
FWCI: 7.01
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
University of California, San Francisco
National Institute of Neurological Disorders and Stroke (R01NS036942)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords: synaptic vesicle recycling, exocytosis, endocytosis, kiss-and-run, single-vesicle imaging, single-molecule tracking, presynaptic terminals, synaptic transmission
MeSH terms: Animals, Cells, Cultured, Endocytosis, Exocytosis, Microscopy, Fluorescence, Synaptic Vesicles, Rats, Sprague-Dawley, Photobleaching, Green Fluorescent Proteins, Rats

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Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode

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