The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Micromagnetic selection of aptamers in microfluidic channels
Open accessAbstract
Aptamers are nucleic acid molecules that have been selected in vitro to bind to their molecular targets with high affinity and specificity. Typically, the systematic evolution of ligands by exponential enrichment (SELEX) process is used for the isolation of specific, high-affinity aptamers. SELEX, however, is an iterative process requiring multiple rounds of selection and amplification that demand significant time and labor. Here, we describe an aptamer discovery system that is rapid, highly efficient, automatable, and applicable to a wide range of targets, based on the integration of magnetic bead-based SELEX process with microfluidics technology. Our microfluidic SELEX (M-SELEX) method exploits a number of unique phenomena that occur at the microscale and implements a design that enables it to manipulate small numbers of beads precisely and isolate high-affinity aptamers rapidly. As a model to demonstrate the efficiency of the M-SELEX process, we describe here the isolation of DNA aptamers that tightly bind to the light chain of recombinant Botulinum neurotoxin type A (with low-nanomolar dissociation constant) after a single round of selection.
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Cites
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
Tuerk, C., & Gold, L. (1990) · Science · doi:10.1126/science.2200121 · Cited by 10,405
In vitro selection of RNA molecules that bind specific ligands
Ellington, A. D., & Szostak, J. W. (1990) · Nature · doi:10.1038/346818a0 · Cited by 9,812
Selection of single-stranded DNA molecules that bind and inhibit human thrombin
Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H., & Toole, J. J. (1992) · Nature · doi:10.1038/355564a0 · Cited by 2,469
Aptamers: An Emerging Class of Therapeutics
Nimjee, S. M., Rusconi, C. P., & Sullenger, B. A. (2005) · Annual Review of Medicine · doi:10.1146/annurev.med.56.062904.144915 · Cited by 937
Electronic Aptamer‐Based Sensors
Willner, I., & Zayats, M. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200604524 · Cited by 818
Label‐Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer‐Based Sensor
Xiao, Y., Lubin, A. A., Heeger, A. J., & Plaxco, K. W. (2005) · Angewandte Chemie International Edition · doi:10.1002/anie.200500989 · Cited by 812
Self-citationDIVERSITY OF OLIGONUCLEOTIDE FUNCTIONS
Gold, L., Polisky, B., Uhlenbeck, O., & Yarus, M. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.003555 · Cited by 805
(1997) · General Pharmacology: The Vascular System · doi:10.1016/s0306-3623(97)89864-3 · Cited by 750
Daniels, D. A., Chen, H., Hicke, B. J., Swiderek, K. M., & Gold, L. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2136683100 · Cited by 567
Cited by
Keefe, A. D., Pai, S., & Ellington, A. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3141 · Cited by 2,289
Nucleic Acid Aptamers for Molecular Diagnostics and Therapeutics: Advances and Perspectives
Li, L., Xu, S., Yan, H., Li, X., Yazd, H. S., Li, X., Huang, T., Cui, C., Jiang, J., & Tan, W. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202003563 · Cited by 432
Empowering Exosomes with Aptamers for Precision Theranostics
Zheng, L., Li, J., Li, Y., Sun, W., Ma, L., Qu, F., & Tan, W. (2025) · Small Methods · doi:10.1002/smtd.202400551 · Cited by 21
Metadata
- Authors
- Lou, X., Qian, J., Xiao, Y., Viel, L., Gerdon, A. E., Lagally, E. T., Atzberger, P., Tarasow, T. M., Heeger, A. J., & Soh, H. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-03-03
- Volume
- 106
- Issue
- 9
- Pages
- 2989-2994
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2017060659
- Cited by
- 349
- References
- 33
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Lou, X., Qian, J., Xiao, Y., Viel, L., Gerdon, A. E., Lagally, E. T., Atzberger, P., Tarasow, T. M., Heeger, A. J., & Soh, H. T. (2009). Micromagnetic selection of aptamers in microfluidic channels. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0813135106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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