Neurotrophin secretion from hippocampal neurons evoked by long-term-potentiation-inducing electrical stimulation patterns
Open accessAbstract
The neurotrophin (NT) brain-derived neurotrophic factor (BDNF) plays an essential role in the formation of long-term potentiation (LTP). Here, we address whether this modulation by BDNF requires its continuous presence, or whether a local increase in BDNF is necessary during a specific time period of LTP initiation. Using electrical field stimulation of primary cultures of hippocampal neurons, we demonstrate that short high-frequency bursts of stimuli that induce LTP evoke also an instantaneous secretion of BDNF. In contrast, stimuli at low frequencies, inducing long-term depression, do not enhance BDNF secretion, suggesting that BDNF is specifically present, and thus required, at the time of LTP induction. The field-stimulation-mediated BDNF secretion depends on the formation of action potentials and is induced by IP(3)-mediated Ca(2+) release from intracellular stores. Experiments, aimed at determining the sites of NT secretion that use NT6, showed similar patterns of surface labeling by field stimulation to those shown previously by high potassium.
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BDNF secretion is not induced by a 1-Hz spike train alone.
“Because BDNF secretion is not induced by a 1-Hz spike train alone (22, 23), our data suggest that the secretion of this neurotrophin is responsive to the synchrony of synaptic input and postsynaptic spikes.”
From Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines (2008) · cites this paper as [22]
The secretion of BDNF is responsive to the synchrony of synaptic input and postsynaptic spikes.
“Because BDNF secretion is not induced by a 1-Hz spike train alone (22, 23), our data suggest that the secretion of this neurotrophin is responsive to the synchrony of synaptic input and postsynaptic spikes.”
From Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines (2008) · cites this paper as [22]
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- Authors
- Gärtner, A., & Staiger, V.
- Affiliations
- Max Planck Institute of Neurobiology (DE)
- Max Planck Society (DE)
- ORCID
- Gärtner, Annette
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2002-04-30
- Volume
- 99
- Issue
- 9
- Pages
- 6386-6391
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC122958
- OpenAlex
- W2071680607
- Cited by
- 195
- References
- 3
- Citations by year
- 2025: 5 · 2024: 7 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 8 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 12 · 2014: 14 · 2013: 13 · 2012: 10
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion, cc-by)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- brain-derived neurotrophic factor, neurotrophin secretion, long-term potentiation, hippocampal neurons, electrical field stimulation, long-term depression, IP3 receptor, intracellular calcium release, action potential, high potassium stimulation, synaptic plasticity
- MeSH terms
- Cyclic AMP, Adenoviridae, Animals, Calcium, Cells, Cultured, Electric Stimulation, Electrophysiology, Enzyme-Linked Immunosorbent Assay, Hippocampus, Microscopy, Electron, Nerve Growth Factors, Neurons, Time Factors, Rats, Wistar, Long-Term Potentiation, Brain-Derived Neurotrophic Factor, Rats
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