The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Structural and functional analysis of the YAP-binding domain of human TEAD2
Open accessAbstract
The Hippo pathway controls organ size and suppresses tumorigenesis in metazoans by blocking cell proliferation and promoting apoptosis. The TEAD1-4 proteins (which contain a DNA-binding domain but lack an activation domain) interact with YAP (which lacks a DNA-binding domain but contains an activation domain) to form functional heterodimeric transcription factors that activate proliferative and prosurvival gene expression programs. The Hippo pathway inhibits the YAP-TEAD hybrid transcription factors by phosphorylating and promoting cytoplasmic retention of YAP. Here we report the crystal structure of the YAP-binding domain (YBD) of human TEAD2. TEAD2 YBD adopts an immunoglobulin-like β-sandwich fold with two extra helix-turn-helix inserts. NMR studies reveal that the TEAD-binding domain of YAP is natively unfolded and that TEAD binding causes localized conformational changes in YAP. In vitro binding and in vivo functional assays define an extensive conserved surface of TEAD2 YBD as the YAP-binding site. Therefore, our studies suggest that a short segment of YAP adopts an extended conformation and forms extensive contacts with a rigid surface of TEAD. Targeting a surface-exposed pocket of TEAD might be an effective strategy to disrupt the YAP-TEAD interaction and to reduce the oncogenic potential of YAP.
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Cites
PHENIX : building new software for automated crystallographic structure determination
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Self-citationYAP1 increases organ size and expands undifferentiated progenitor cells
Camargo, F. D., Gokhale, S., Johnnidis, J. B., Fu, D., Bell, G. W., Jaenisch, R., & Brummelkamp, T. R. (2007) · Current Biology · doi:10.1016/j.cub.2007.10.039 · Cited by 1,252
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Wu, S., Liu, Y., Zheng, Y., Dong, J., & Pan, D. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.01.007 · Cited by 640 · cited 1×
“Similar to the fly pathway, the effectors of the mammalian Hippo pathway are transcription factors, including YAP (Yes-associated protein), and TEAD, the mammalian homologs of Yki and Sd, respectively (16, 18, 22, 23).”
BackgroundSelf-citationThe TEAD/TEF family of transcription factor Scalloped mediates Hippo signaling in organ size control
Zhang, L., Ren, F., Zhang, Q., Chen, Y., Wang, B., & Jiang, J. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.01.006 · Cited by 630
The Salvador–Warts–Hippo pathway — an emerging tumour-suppressor network
Harvey, K., & Tapon, N. (2007) · Nature Reviews Cancer · doi:10.1038/nrc2070 · Cited by 628
Cited by
The Hippo Signaling Pathway in Development and Cancer
Pan, D. (2010) · Developmental Cell · doi:10.1016/j.devcel.2010.09.011 · Cited by 2,269
Self-citationYAP/TAZ at the Roots of Cancer
Zanconato, F., Cordenonsi, M., & Piccolo, S. (2016) · Cancer Cell · doi:10.1016/j.ccell.2016.05.005 · Cited by 1,970
The Hippo Signaling Pathway in Development and Disease
Zheng, Y., & Pan, D. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.06.003 · Cited by 867
Self-citationCellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway
Mo, J.-S., Meng, Z., Kim, Y. C., Park, H. W., Hansen, C. G., Kim, S., Lim, D.-S., & Guan, K.-L. (2015) · Nature Cell Biology · doi:10.1038/ncb3111 · Cited by 557
YAP1 Exerts Its Transcriptional Control via TEAD-Mediated Activation of Enhancers
Stein, C., Bardet, A. F., Roma, G., Bergling, S., Clay, I., Ruchti, A., Agarinis, C., Schmelzle, T., Bouwmeester, T., Schübeler, D., et al. (2015) · PLOS Genetics · doi:10.1371/journal.pgen.1005465 · Cited by 414
Metadata
- Authors
- Tian, W., Yu, J., Tomchick, D. R., Pan, D., & Luo, X.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-04-05
- Volume
- 107
- Issue
- 16
- Pages
- 7293-7298
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2091198887
- Cited by
- 169
- References
- 7
- FWCI
- 3.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Tian, W., Yu, J., Tomchick, D. R., Pan, D., & Luo, X. (2010). Structural and functional analysis of the YAP-binding domain of human TEAD2. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1000293107
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