The TEAD/TEF Family Protein Scalloped Mediates Transcriptional Output of the Hippo Growth-Regulatory Pathway
Open accessAbstract
The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.
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YAP predominantly exerts its transcriptional functions through interaction with the TEAD family of transcription factors.
“YAP predominantly exerts its transcriptional functions through interaction with the TEAD family of transcription factors, thereby promoting the expression of target genes12,13.”
From HBO1 functions as an epigenetic barrier to hepatocyte plasticity and reprogramming during liver injury (2025) · cites this paper as [12]
The Introduction reports that YAP interacts with TEAD/TEF transcription factors, and the Discussion suggests that the growth-regulatory activity of the fly Sd–Yki complex is conserved in mammals. The paper does not establish that YAP uses TEAD for *most* of its transcriptional functions; it also names other YAP-interacting transcription factors. Page numbers are unavailable in our copy. (LLM, conf=0.84)
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Toll Receptor-Mediated Hippo Signaling Controls Innate Immunity in Drosophila
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The Hippo Pathway in Liver Homeostasis and Pathophysiology
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- Authors
- Wu, S., Liu, Y., Zheng, Y., Dong, J., & Pan, D.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Pan, Duojia
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2008-03
- Volume
- 14
- Issue
- 3
- Pages
- 388-398
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W1978746441
- Cited by
- 640
- References
- 47
- Citations by year
- 2026: 20 · 2025: 28 · 2024: 35 · 2023: 27 · 2022: 30 · 2021: 34 · 2020: 43 · 2019: 35 · 2018: 38 · 2017: 32 · 2016: 42 · 2015: 44 · 2014: 42 · 2013: 37 · 2012: 37
- FWCI
- 16.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1964 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Developmental Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (EY015708)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- Hippo signaling pathway, Scalloped, Yorkie, DIAP1, transcriptional regulation, tissue growth regulation, tissue overgrowth, enhancers, protein-protein interaction, Drosophila
- MeSH terms
- YAP-Signaling Proteins, Hippo Kinases, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, DNA, Drosophila, Epistasis, Genetic, Models, Biological, Molecular Sequence Data, Mutation, Nuclear Proteins, Organ Size, Transcription Factors, Transcription, Genetic, Wings, Animal, Signal Transduction, Trans-Activators, Genes, Insect, Protein Serine-Threonine Kinases, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Gene Dosage, Drosophila Proteins, Animals, Genetically Modified, Intracellular Signaling Peptides and Proteins, Inhibitor of Apoptosis Proteins
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