Cited by 640Hippo pathway signaling and YAP/TAZDevelopmental Biology and Gene RegulationPI3K/AKT/mTOR signaling in cancer

The TEAD/TEF Family Protein Scalloped Mediates Transcriptional Output of the Hippo Growth-Regulatory Pathway

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Wu, Shian, Liu, Yi, Zheng, Yonggang, Dong, Jixin, Pan, Duojia·2008·Developmental Cell

Abstract

The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.

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Crossref · 10.1016/j.devcel.2008.01.007

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  • YAP predominantly exerts its transcriptional functions through interaction with the TEAD family of transcription factors.

    “YAP predominantly exerts its transcriptional functions through interaction with the TEAD family of transcription factors, thereby promoting the expression of target genes12,13.”

    From HBO1 functions as an epigenetic barrier to hepatocyte plasticity and reprogramming during liver injury (2025) · cites this paper as [12]

    The Introduction reports that YAP interacts with TEAD/TEF transcription factors, and the Discussion suggests that the growth-regulatory activity of the fly Sd–Yki complex is conserved in mammals. The paper does not establish that YAP uses TEAD for *most* of its transcriptional functions; it also names other YAP-interacting transcription factors. Page numbers are unavailable in our copy. (LLM, conf=0.84)

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Authors
Wu, S., Liu, Y., Zheng, Y., Dong, J., & Pan, D.
Affiliations
Johns Hopkins University (US)
Corresponding author
Pan, Duojia
Venue
Developmental Cell
Publisher
Elsevier BV
Published
2008-03
Volume
14
Issue
3
Pages
388-398
ISSN
1534-5807 (ISSN-L)
OpenAlex
W1978746441
Cited by
640
References
47
Citations by year
2026: 20 · 2025: 28 · 2024: 35 · 2023: 27 · 2022: 30 · 2021: 34 · 2020: 43 · 2019: 35 · 2018: 38 · 2017: 32 · 2016: 42 · 2015: 44 · 2014: 42 · 2013: 37 · 2012: 37
FWCI
16.93
Type
journal article
Language
en
Open access
Locations
  • Developmental Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (EY015708)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
Hippo signaling pathway, Scalloped, Yorkie, DIAP1, transcriptional regulation, tissue growth regulation, tissue overgrowth, enhancers, protein-protein interaction, Drosophila
MeSH terms
YAP-Signaling Proteins, Hippo Kinases, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, DNA, Drosophila, Epistasis, Genetic, Models, Biological, Molecular Sequence Data, Mutation, Nuclear Proteins, Organ Size, Transcription Factors, Transcription, Genetic, Wings, Animal, Signal Transduction, Trans-Activators, Genes, Insect, Protein Serine-Threonine Kinases, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Gene Dosage, Drosophila Proteins, Animals, Genetically Modified, Intracellular Signaling Peptides and Proteins, Inhibitor of Apoptosis Proteins

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