The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Two-photon single-cell optogenetic control of neuronal activity by sculpted light
Open accessAbstract
Recent advances in optogenetic techniques have generated new tools for controlling neuronal activity, with a wide range of neuroscience applications. The most commonly used approach has been the optical activation of the light-gated ion channel channelrhodopsin-2 (ChR2). However, targeted single-cell-level optogenetic activation with temporal precessions comparable to the spike timing remained challenging. Here we report fast (≤1 ms), selective, and targeted control of neuronal activity with single-cell resolution in hippocampal slices. Using temporally focused laser pulses (TEFO) for which the axial beam profile can be controlled independently of its lateral distribution, large numbers of channels on individual neurons can be excited simultaneously, leading to strong (up to 15 mV) and fast (≤1 ms) depolarizations. Furthermore, we demonstrated selective activation of cellular compartments, such as dendrites and large presynaptic terminals, at depths up to 150 μm. The demonstrated spatiotemporal resolution and the selectivity provided by TEFO allow manipulation of neuronal activity, with a large number of applications in studies of neuronal microcircuit function in vitro and in vivo.
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Cites
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Phasic Firing in Dopaminergic Neurons Is Sufficient for Behavioral Conditioning
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Compartmentalized dendritic plasticity and input feature storage in neurons
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Cited by
Optogenetics in neural systems
Yizhar, O., Fenno, L. E., Davidson, T. J., Mogri, M., & Deisseroth, K. (2011) · Neuron · doi:10.1016/j.neuron.2011.06.004 · Cited by 1,881
The development and application of optogenetics
Fenno, L., Yizhar, O., & Deisseroth, K. (2011) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-061010-113817 · Cited by 1,822
Remote Control of Neuronal Signaling
Rogan, S. C., & Roth, B. L. (2011) · Pharmacological Reviews · doi:10.1124/pr.110.003020 · Cited by 339
Two-photon optogenetic toolbox for fast inhibition, excitation and bistable modulation
Prakash, R., Yizhar, O., Grewe, B., Ramakrishnan, C., Wang, N., Goshen, I., Packer, A. M., Peterka, D. S., Yuste, R., Schnitzer, M. J., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2215 · Cited by 334
Genetic Approaches to Neural Circuits in the Mouse
Josh Huang, Z., & Zeng, H. (2013) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062012-170307 · Cited by 200
Metadata
- Authors
- Andrasfalvy, B. K., Zemelman, B. V., Tang, J., & Vaziri, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-06-11
- Volume
- 107
- Issue
- 26
- Pages
- 11981-11986
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1997247894
- Cited by
- 205
- References
- 9
- FWCI
- 7.96
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Andrasfalvy, B. K., Zemelman, B. V., Tang, J., & Vaziri, A. (2010). Two-photon single-cell optogenetic control of neuronal activity by sculpted light. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1006620107
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