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Cited by 262Microbial Fuel Cells and BioremediationBacterial Genetics and BiotechnologyMetalloenzymes and iron-sulfur proteins

Bacterial Na + -translocating ferredoxin:NAD + oxidoreductase

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Biegel, Eva, Müller, Volker·2010·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1010318107PMC PMC2964206
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Abstract

The anaerobic acetogenic bacterium Acetobacterium woodii carries out a unique type of Na + -motive, anaerobic respiration with caffeate as electron acceptor, termed “caffeate respiration.” Central, and so far the only identified membrane-bound reaction in this respiration pathway, is a ferredoxin:NAD + oxidoreductase (Fno) activity. Here we show that inverted membrane vesicles of A. woodii couple electron transfer from reduced ferredoxin to NAD + with the transport of Na + from the outside into the lumen of the vesicles. Na + transport was electrogenic, and accumulation was inhibited by sodium ionophores but not protonophores, demonstrating a direct coupling of Fno activity to Na + transport. Results from inhibitor studies are consistent with the hypothesis that Fno activity coupled to Na + translocation is catalyzed by the Rnf complex, a membrane-bound, iron–sulfur and flavin-containing electron transport complex encoded by many bacterial and some archaeal genomes. Fno is a unique type of primary Na + pump and represents an early evolutionary mechanism of energy conservation that expands the redox range known to support life. In addition, it explains the lifestyle of many anaerobic bacteria and gives a mechanistic explanation for the enigma of the energetic driving force for the endergonic reduction of ferredoxin with NADH plus H + as reductant in a number of aerobic bacteria.

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Crossref · 10.1073/pnas.1010318107

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  • Fdred is the substrate for a membrane-bound protein called Rnf that generates NADH and pumps about one Na+ ion per electron transferred, thus generating the ion gradient harnessed by the ATPase, which requires about 4 Na+ ions per ATP.

    “Fdred is the substrate for a membrane-bound protein called Rnf [147] that generates NADH and pumps, in the process, about one Na+ ion per electron transferred, thus generating the ion gradient that is harnessed by the ATPase, which requires about 4 Na+ ions per ATP.”

    From Early bioenergetic evolution (2013) · cites this paper as [147]

  • Oxidation of Fdred by a pumping complex such as Rnf or the Ech hydrogenase couples the electron bifurcation mechanism to chemiosmotic energy conservation.

    “Oxidation of Fdred by a pumping complex such as Rnf [147] or the Ech hydrogenase [159] couples the electron bifurcation mechanism to chemiosmotic energy conservation (figure 4).”

    From Early bioenergetic evolution (2013) · cites this paper as [147]

  • Electron transfer from ferredoxin to NAD+ drives the extrusion of Na+ via Rnf.

    “Electron transfer from ferredoxin to NAD+ drives the extrusion of Na+ via Rnf [147].”

    From Early bioenergetic evolution (2013) · cites this paper as [147]

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Authors
Biegel, E., & Müller, V.
Affiliations
Goethe University Frankfurt (DE)
Corresponding author
Müller, Volker
ORCID
Müller, Volker
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2010-10-04
Volume
107
Issue
42
Pages
18138-18142
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1010318107
PMC
PMC2964206
OpenAlex
W2035252706
Cited by
262
References
1
Citations by year
2026: 7 · 2025: 7 · 2024: 7 · 2023: 15 · 2022: 18 · 2021: 14 · 2020: 19 · 2019: 21 · 2018: 14 · 2017: 10 · 2016: 28 · 2015: 25 · 2014: 21 · 2013: 20 · 2012: 17
FWCI
17.22
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
Keywords
Acetobacterium woodii, anaerobic respiration, sodium transport, electrogenic transport, inverted membrane vesicles, Rnf complex, iron-sulfur flavoprotein, electron transfer, energy conservation, anaerobic bacteria
MeSH terms
Biological Evolution, Ferredoxins, NADH Dehydrogenase, Sodium, Ion Transport, Acetobacterium

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