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Cited by 80Anaerobic Digestion and Biogas ProductionMicrobial metabolism and enzyme functionMicrobial Fuel Cells and Bioremediation

The complete genome sequence of Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic acetogens

Open access
Poehlein, Anja, Cebulla, Martin, Ilg, Marcus M., Bengelsdorf, Frank R., Schiel-Bengelsdorf, Bettina, Whited, Gregg, Andreesen, Jan R., Gottschalk, Gerhard +2 more·2015·mBio
DOI 10.1128/mbio.01168-15PMC PMC4600107
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Abstract

UNLABELLED: Clostridium aceticum was the first isolated autotrophic acetogen, converting CO2 plus H2 or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.7 kbp. Sequence analysis revealed major differences from other autotrophic acetogens. C. aceticum contains an Rnf complex for energy conservation (via pumping protons or sodium ions). Such systems have also been found in C. ljungdahlii and Acetobacterium woodii. However, C. aceticum also contains a cytochrome, as does Moorella thermoacetica, which has been proposed to be involved in the generation of a proton gradient. Thus, C. aceticum seems to represent a link between Rnf- and cytochrome-containing autotrophic acetogens. In C. aceticum, however, the cytochrome is probably not involved in an electron transport chain that leads to proton translocation, as no genes for quinone biosynthesis are present in the genome. IMPORTANCE: Autotrophic acetogenic bacteria are receiving more and more industrial focus, as CO2 plus H2 as well as syngas are interesting new substrates for biotechnological processes. They are both cheap and abundant, and their use, if it results in sustainable products, also leads to reduction of greenhouse gases. Clostridium aceticum can use both gas mixtures, is phylogenetically not closely related to the commonly used species, and may thus become an even more attractive workhorse. In addition, its energy metabolism, which is characterized here, and the ability to synthesize cytochromes might offer new targets for improving the ATP yield by metabolic engineering and thus allow use of C. aceticum for production of compounds by pathways that currently present challenges for energy-limited acetogens.

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  • Whole genome sequences have been elucidated for a range of acetogens including C. autoethanogenum, C. ljungdahlii, C. aceticum, M. thermoacetica, A. woodii, and E. limosum.

    “These include elucidation of whole genome sequences for a range of acetogens including C. autoethanogenum (Brown et al., 2014; Humphreys et al., 2015; Utturkar et al., 2015), C. ljungdahlii (Köpke et al., 2010), C. aceticum (Poehlein et al., 2015), M. thermoacetica (Pierce et al., 2008), A. woodii (Poehlein et al., 2012), and E. limosum (Roh et al., 2011);”

    From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Poehlein et al., 2015

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  1. [15]Braun M 1981 Charakterisierung von anaeroben autotrophen Essigsäurebildnern und Untersuchungen zur Essigsäurebildung aus Wasserstoff und Kohlendioxid durch Clostridium aceticum. Ph.D. thesis University of Göttingen, Göttingen, Germany.

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Authors
Poehlein, A., Cebulla, M., Ilg, M. M., Bengelsdorf, F. R., Schiel-Bengelsdorf, B., Whited, G., Andreesen, J. R., Gottschalk, G., Daniel, R., & Dürre, P.
Affiliations
  • University of Göttingen (DE)
  • Universität Ulm (DE)
  • Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Albert-Einstein-Allee 11, Ulm, Germany
  • Genencor Division, Danisco US Inc., Palo Alto, California, USA
  • Martin Luther University Halle-Wittenberg (DE)
Corresponding author
Dürre, Peter
ORCID
Venue
mBio
Publisher
American Society for Microbiology
Published
2015-10-30
Volume
6
Issue
5
Pages
e01168-15
ISSN
  • 2150-7511 (ISSN-L)
  • 2161-2129
DOI
10.1128/mbio.01168-15
PMC
PMC4600107
OpenAlex
W2141949626
Cited by
80
References
55
Citations by year
2025: 5 · 2024: 2 · 2023: 7 · 2022: 6 · 2021: 5 · 2020: 13 · 2019: 5 · 2018: 15 · 2017: 8 · 2016: 11 · 2015: 3
FWCI
16.78
Type
journal article
Language
en
Open access
Licence
Locations
  • mBio (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by-nc-sa)
  • GRO (submittedVersion, cc-by-nc-sa)
Funders
Bundesministerium für Bildung und Forschung
Topics
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
Keywords
Clostridium aceticum, genome sequence, Rnf complex, cytochromes, proton translocation, energy conservation, plasmids, syngas conversion, acetate production, quinone synthesis, metabolic engineering, Clostridium ljungdahlii, Acetobacterium woodii, Moorella thermoacetica
MeSH terms
Chromosomes, Bacterial, Clostridium, Cytochromes, DNA, Bacterial, Energy Metabolism, Molecular Sequence Data, Plasmids, Genome, Bacterial, Sequence Analysis, DNA, Metabolic Networks and Pathways

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