The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.
Open accessAbstract
Algae have reemerged as potential next-generation feedstocks for biofuels, but strain improvement and progress in algal biology research have been limited by the lack of advanced molecular tools for most eukaryotic microalgae. Here we describe the development of an efficient transformation method for Nannochloropsis sp., a fast-growing, unicellular alga capable of accumulating large amounts of oil. Moreover, we provide additional evidence that Nannochloropsis is haploid, and we demonstrate that insertion of transformation constructs into the nuclear genome can occur by high-efficiency homologous recombination. As examples, we generated knockouts of the genes encoding nitrate reductase and nitrite reductase, resulting in strains that were unable to grow on nitrate and nitrate/nitrite, respectively. The application of homologous recombination in this industrially relevant alga has the potential to rapidly advance algal functional genomics and biotechnology.
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Cites
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879
Valuable Products from Biotechnology of Microalgae
Pulz, O., & Gross, W. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1647-x · Cited by 2,132 · cited 1×
“Many algae have high photoautotrophic growth rates and can accumulate more than half their dry weight biomass as lipids (5), including triacylglycerol and a number of high-value pharmaceutical and nutraceutical products (6).”
BackgroundGenetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
Keeling, P. J., Burger, G., Durnford, D. G., Lang, B. F., Lee, R. W., Pearlman, R. E., Roger, A. J., & Gray, M. W. (2005) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2005.09.005 · Cited by 579
Future prospects of microalgal biofuel production systems
Stephens, E., Ross, I. L., Mussgnug, J. H., Wagner, L. D., Borowitzka, M. A., Posten, C., Kruse, O., & Hankamer, B. (2010) · Trends in Plant Science · doi:10.1016/j.tplants.2010.06.003 · Cited by 340
Cited by
High-value products from microalgae—their development and commercialisation
Borowitzka, M. A. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-9983-9 · Cited by 1,585
Microalgae as a future food source
Torres-Tiji, Y., Fields, F. J., & Mayfield, S. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107536 · Cited by 559
Vieler, A., Wu, G., Tsai, C.-H., Bullard, B., Cornish, A. J., Harvey, C., Reca, I.-B., Thornburg, C., Achawanantakun, R., Buehl, C. J., et al. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1003064 · Cited by 458
Self-citationGenome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Daboussi, F., Leduc, S., Maréchal, A., Dubois, G., Guyot, V., Perez-Michaut, C., Amato, A., Falciatore, A., Juillerat, A., Beurdeley, M., et al. (2014) · Nature Communications · doi:10.1038/ncomms4831 · Cited by 455
CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
Shin, S.-E., Lim, J.-M., Koh, H. G., Kim, E. K., Kang, N. K., Jeon, S., Kwon, S., Shin, W.-S., Lee, B., Hwangbo, K., et al. (2016) · Scientific Reports · doi:10.1038/srep27810 · Cited by 423
Metadata
- Authors
- Kilian, O., Benemann, C. S. E., Niyogi, K. K., & Vick, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-11-28
- Volume
- 108
- Issue
- 52
- Pages
- 21265-21269
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W4247025394
- Cited by
- 421
- References
- 5
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Kilian, O., Benemann, C. S. E., Niyogi, K. K., & Vick, B. (2011). High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1105861108
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