Dynamics of the leading process, nucleus, and Golgi apparatus of migrating cortical interneurons in living mouse embryos
Open accessAbstract
Precisely arranged cytoarchitectures such as layers and nuclei depend on neuronal migration, of which many in vitro studies have revealed the mode and underlying mechanisms. However, how neuronal migration is achieved in vivo remains unknown. Here we established an imaging system that allows direct visualization of cortical interneuron migration in living mouse embryos. We found that during nucleokinesis, translocation of the Golgi apparatus either precedes or occurs in parallel to that of the nucleus, suggesting the existence of both a Golgi/centrosome-dependent and -independent mechanism of nucleokinesis. Changes in migratory direction occur when the nucleus enters one of the leading process branches, which is accompanied by the retraction of other branches. The nucleus occasionally swings between two branches before translocating into one of them, the occurrence of which is most often preceded by Golgi apparatus translocation into that branch. These in vivo observations provide important insight into the mechanisms of neuronal migration and demonstrate the usefulness of our system for studying dynamic events in living animals.
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Interneurons born in the MGE, CGE and preoptic region use largely the same cellular mechanisms to move to the cortex and show strong transcriptional similarity at that stage.
“Interneurons from the MGE, CGE and preoptic region use essentially the same cellular mechanisms to translocate to the cortex (Marín et al., 2006; Martini et al., 2009; Yanagida et al., 2012), and it is therefore perhaps not surprising that they exhibit great transcriptional similarity at this stage (Mayer et al., 2018).”
From Development and Functional Diversification of Cortical Interneurons (2018) · cites this paper as Yanagida et al., 2012
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Development and Functional Diversification of Cortical Interneurons
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- Authors
- Yanagida, M., Miyoshi, R., Toyokuni, R., Zhu, Y., & Murakami, F.
- Affiliations
- The University of Osaka (JP)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-09-24
- Volume
- 109
- Issue
- 41
- Pages
- 16737-16742
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3478636
- OpenAlex
- W1970232029
- Cited by
- 58
- References
- 44
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 10 · 2019: 1 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 6 · 2013: 3
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (24650166, 23570226)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- neuronal migration, cortical interneurons, leading process, centrosome, migratory direction, mouse embryo, in vivo imaging
- MeSH terms
- Animals, Cell Movement, Cell Nucleus, Cerebral Cortex, Embryo, Mammalian, Female, Golgi Apparatus, Immunohistochemistry, Interneurons, Kinetics, Male, Mice, Inbred ICR, Pregnancy, Time Factors, Centrosome, Microscopy, Confocal, Microscopy, Fluorescence, Multiphoton, Green Fluorescent Proteins, Mice
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