Cited by 147Axon Guidance and Neuronal SignalingNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

Biased selection of leading process branches mediates chemotaxis during tangential neuronal migration

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Martini, Francisco J., Valiente, Manuel, López Bendito, Guillermina, Szabó, Gábor, Moya, Fernando, Valdeolmillos, Miguel, Marín, Oscar·2009·Development

Abstract

Current models of chemotaxis during neuronal migration and axon guidance propose that directional sensing relies on growth cone dynamics. According to this view, migrating neurons and growing axons are guided to their correct targets by steering the growth cone in response to attractive and repulsive cues. Here, we have performed a detailed analysis of the dynamic behavior of individual neurons migrating tangentially in telencephalic slices using high-resolution time-lapse videomicroscopy. We found that cortical interneurons consistently display branched leading processes as part of their migratory cycle, a feature that seems to be common to many other populations of GABAergic neurons in the brain and spinal cord. Analysis of the migratory behavior of individual cells suggests that interneurons respond to chemoattractant signals by generating new leading process branches that are better aligned with the source of the gradient, and not by reorienting previously existing branches. Moreover, experimental evidence revealed that guidance cues influence the angle at which new branches emerge. This model is further supported by pharmacological experiments in which inhibition of branching blocked chemotaxis, suggesting that this process is an essential component of the mechanism controlling directional guidance. These results reveal a novel guidance mechanism during neuronal migration that might be extensively used in brain development.

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Crossref · 10.1242/dev.025502

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Authors
Martini, F. J., Valiente, M., López Bendito, G., Szabó, G., Moya, F., Valdeolmillos, M., & Marín, O.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, 03550 Sant Joan d'Alacant, Spain.
  • Institute of Experimental Medicine, 1083 Budapest, Hungary.
Venue
Development
Published
2009-01-01
Volume
136
Issue
1
Pages
41-50
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W1985485745
Cited by
147
References
36
Citations by year
2026: 3 · 2025: 7 · 2024: 7 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 13 · 2019: 7 · 2018: 15 · 2017: 10 · 2016: 7 · 2015: 4 · 2014: 11 · 2013: 11 · 2012: 11
FWCI
3.46
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
neuronal migration, chemotaxis, tangential migration, cortical interneurons, GABAergic neurons, axon guidance, growth cone dynamics, chemoattractant signaling, directional sensing, telencephalon, brain development
MeSH terms
Amides, Animals, Central Nervous System, Chlorocebus aethiops, Chemotaxis, Interneurons, Nerve Tissue Proteins, Neurons, Pyridines, Selection, Genetic, COS Cells, Neuregulin-1, Cell Surface Extensions, Mice

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