Small-angle X-ray scattering-derived structure of the HIV-1 5′ UTR reveals 3D tRNA mimicry
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Significance: A highly conserved region of the HIV-1 RNA genome is responsible for regulating numerous steps of the retroviral life cycle, including initiation of reverse transcription. A complete understanding of the mechanisms controlling HIV-1 replication requires structural characterization of this RNA; unfortunately, however, its large size and conformational flexibility makes common methods of solving structures, such as X-ray crystallography and NMR, exceedingly difficult. The present study uses a solution technique, small-angle X-ray scattering coupled with computational molecular modeling, to characterize three ∼100-nucleotide RNAs that play central roles in HIV-1 replication. One of these domains mimics the L-shaped fold of tRNA, providing a structural basis for understanding how this genomic RNA coordinates interactions with a tRNA-binding host factor to facilitate initiation of reverse transcription.
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The HIV-1 5'UTR contains a tRNA-like element (TLE) that resembles the anticodon loop of tRNA Lys,3 in both sequence and 3D structure.
“The 5ЈUTR of the HIV-1 genome contains a tRNA-like element (TLE) that resembles the anticodon loop of tRNA Lys,3 in both sequence and 3D structure (70 -72).”
From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [71]
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- Authors
- Jones, C. P., Cantara, W. A., Olson, E. D., & Musier-Forsyth, K.
- Affiliations
- The Ohio State University (US)
- Department of Chemistry and Biochemistry, Center for Retrovirus Research, and Center for RNA Biology, The Ohio State University, Columbus, OH 43210
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2014-02-18
- Volume
- 111
- Issue
- 9
- Pages
- 3395-3400
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3948283
- OpenAlex
- W2099779927
- Cited by
- 71
- References
- 60
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 8 · 2021: 9 · 2020: 9 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 12 · 2015: 4 · 2014: 3
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy
- Ohio State University
- Pelotonia
- National Institutes of Health
- National Institute of General Medical Sciences (T32GM008512, R01GM105404, R01GM113887)
- National Institute of Allergy and Infectious Diseases (R01AI077387)
- Basic Energy Sciences
- Biological and Environmental Research
- Lawrence Berkeley National Laboratory
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- RNA tertiary structure, tRNA mimicry, primer binding site, RNA conformational dynamics
- MeSH terms
- Base Sequence, Chromatography, Gel, Models, Molecular, Molecular Sequence Data, RNA, Transfer, HIV-1, Base Pairing, 5' Untranslated Regions, Scattering, Small Angle, Molecular Dynamics Simulation
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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