Cited by 71RNA and protein synthesis mechanismsHIV Research and TreatmentDNA and Nucleic Acid Chemistry

Small-angle X-ray scattering-derived structure of the HIV-1 5′ UTR reveals 3D tRNA mimicry

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Jones, Christopher P., Cantara, William A., Olson, Erik D., Musier-Forsyth, Karin·2014·Proceedings of the National Academy of Sciences

Abstract

Significance: A highly conserved region of the HIV-1 RNA genome is responsible for regulating numerous steps of the retroviral life cycle, including initiation of reverse transcription. A complete understanding of the mechanisms controlling HIV-1 replication requires structural characterization of this RNA; unfortunately, however, its large size and conformational flexibility makes common methods of solving structures, such as X-ray crystallography and NMR, exceedingly difficult. The present study uses a solution technique, small-angle X-ray scattering coupled with computational molecular modeling, to characterize three ∼100-nucleotide RNAs that play central roles in HIV-1 replication. One of these domains mimics the L-shaped fold of tRNA, providing a structural basis for understanding how this genomic RNA coordinates interactions with a tRNA-binding host factor to facilitate initiation of reverse transcription.

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Crossref · 10.1073/pnas.1319658111

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  • The HIV-1 5'UTR contains a tRNA-like element (TLE) that resembles the anticodon loop of tRNA Lys,3 in both sequence and 3D structure.

    “The 5ЈUTR of the HIV-1 genome contains a tRNA-like element (TLE) that resembles the anticodon loop of tRNA Lys,3 in both sequence and 3D structure (70 -72).”

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Authors
Jones, C. P., Cantara, W. A., Olson, E. D., & Musier-Forsyth, K.
Affiliations
  • The Ohio State University (US)
  • Department of Chemistry and Biochemistry, Center for Retrovirus Research, and Center for RNA Biology, The Ohio State University, Columbus, OH 43210
Venue
Proceedings of the National Academy of Sciences
Published
2014-02-18
Volume
111
Issue
9
Pages
3395-3400
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2099779927
Cited by
71
References
60
Citations by year
2026: 2 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 8 · 2021: 9 · 2020: 9 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 12 · 2015: 4 · 2014: 3
FWCI
2.87
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Energy
  • Ohio State University
  • Pelotonia
  • National Institutes of Health
  • National Institute of General Medical Sciences (T32GM008512, R01GM105404, R01GM113887)
  • National Institute of Allergy and Infectious Diseases (R01AI077387)
  • Basic Energy Sciences
  • Biological and Environmental Research
  • Lawrence Berkeley National Laboratory
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
RNA tertiary structure, tRNA mimicry, primer binding site, RNA conformational dynamics
MeSH terms
Base Sequence, Chromatography, Gel, Models, Molecular, Molecular Sequence Data, RNA, Transfer, HIV-1, Base Pairing, 5' Untranslated Regions, Scattering, Small Angle, Molecular Dynamics Simulation

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