Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes

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RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

Mutations affecting segment number and polarity in Drosophila

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One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

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High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells

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RNA-programmed genome editing in human cells

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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

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An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases

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Rabs in Signaling and Embryonic Development

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Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes

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Increased acetylation of microtubules rescues human tau-induced microtubule defects and neuromuscular junction abnormalities in Drosophila

Mao, C., Wen, X., Jin, S., & Zhang, Y. (2017) · Disease Models & Mechanisms · doi:10.1242/dmm.028316 · Cited by 30

Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche

Patel, A., Wu, Y., Han, X., Su, Y. P., Maugel, T. K., Shroff, H., & Roy, S. (2022) · Nature Communications · doi:10.1038/s41467-022-28587-z · Cited by 32

Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching

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mRNA localization in theDrosophilagermline

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CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

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Transneuronal Dpr12/DIP‐δ interactions facilitate compartmentalized dopaminergic innervation of Drosophila mushroom body axons

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Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia

Yap, Z. Y., Efthymiou, S., Seiffert, S., Parra, K. V., Lee, S., Nasca, A., Maroofian, R., Schrauwen, I., Pendziwiat, M., Jung, S., et al. (2021) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2021.11.003 · Cited by 32

A blow to the fly — Lucilia cuprina draft genome and transcriptome to support advances in biology and biotechnology

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Application of the CRISPR gene‐editing technique in insect functional genome studies – a review

Cui, Y., Sun, J., & Yu, L. (2017) · Entomologia Experimentalis et Applicata · doi:10.1111/eea.12530 · Cited by 31

The auxin‐inducible degradation system enables conditional PERIOD protein depletion in the nervous system of Drosophila melanogaster

Chen, W., Werdann, M., & Zhang, Y. (2018) · FEBS Journal · doi:10.1111/febs.14677 · Cited by 26

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jin, J., Xu, Y., Huo, L., Ma, L., Scott, A. W., Pizzi, M. P., Li, Y., Wang, Y., Yao, X., Song, S., et al. (2020) · PLoS ONE · doi:10.1371/journal.pone.0228910 · Cited by 31

Copy number changes in co-expressed odorant receptor genes enable selection for sensory differences in drosophilid species

Auer, T. O., Álvarez-Ocaña, R., Cruchet, S., Benton, R., & Arguello, J. R. (2022) · Nature Ecology & Evolution · doi:10.1038/s41559-022-01830-y · Cited by 31

Unique patterns of organization and migration of FGF-expressing cells during Drosophila morphogenesis

Du, L., Zhou, A., Patel, A., Rao, M., Anderson, K. C., & Roy, S. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.05.009 · Cited by 31

Optogenetic activation of SIFamide (SIFa) neurons induces a complex sleep-promoting effect in the fruit fly Drosophila melanogaster

Huang, H., Possidente, D. R., & Vecsey, C. G. (2021) · Physiology & Behavior · doi:10.1016/j.physbeh.2021.113507 · Cited by 30

Systematic analysis of RhoGEF/GAP localizations uncovers regulators of mechanosensing and junction formation during epithelial cell division

di Pietro, F., Osswald, M., Heras, J. M. D. L., Cristo, I., López-Gay, J. M., Wang, Z., Pelletier, S., Gaugué, I., Leroy, A., Martin, C. A., et al. (2023) · Current Biology · doi:10.1016/j.cub.2023.01.028 · Cited by 30

Lapsyn controls branch extension and positioning of astrocyte-like glia in the Drosophila optic lobe

Richier, B., de Miguel, C., Mackensen, S., & Salecker, I. (2017) · Nature Communications · doi:10.1038/s41467-017-00384-z · Cited by 30

Harnessing model organisms to study insecticide resistance

Perry, T., & Batterham, P. (2018) · Current Opinion in Insect Science · doi:10.1016/j.cois.2018.03.005 · Cited by 30

A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues

Huynh, N., Zeng, J., Liu, W., & King‐Jones, K. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200539 · Cited by 30

Conditional Synaptic Vesicle Markers forDrosophila

Williams, J. L., Shearin, H. K., & Stowers, R. S. (2019) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200975 · Cited by 30

A programmable sequence of reporters for lineage analysis

García‐Marqués, J., Espinosa-Medina, I., Ku, K., Yang, C.-P., Koyama, M., Yu, H., & Lee, T. (2020) · Nature Neuroscience · doi:10.1038/s41593-020-0676-9 · Cited by 30

Engineered reproductively isolated species drive reversible population replacement

Buchman, A. B., Shriner, I., Yang, T., Liu, J., Antoshechkin, I. A., Marshall, J. M., Perry, M., & Akbari, O. S. (2021) · Nature Communications · doi:10.1038/s41467-021-23531-z · Cited by 30

The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity

Sauvola, C. W., Akbergenova, Y., Cunningham, K. L., Aponte-Santiago, N. A., & Littleton, J. T. (2021) · eLife · doi:10.7554/elife.72841 · Cited by 30

An Enhanced Gene Targeting Toolkit forDrosophila: Golic+

Chen, H.-M., Huang, Y., Pfeiffer, B. D., Yao, X., & Lee, T. (2015) · Genetics · doi:10.1534/genetics.114.173716 · Cited by 30

Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids

Andersson-Rolf, A., Merenda, A., Mustata, R. C., Li, T., Dietmann, S., & Koo, B. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.10.016 · Cited by 27

CRISPR/Cas9-mediated deletion of EcMIH shortens metamorphosis time from mysis larva to postlarva of Exopalaemon carinicauda

Zhang, J., Song, F., Sun, Y., Yu, K., & Xiang, J. (2018) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2018.04.002 · Cited by 30

Duox activation in Drosophila Malpighian tubules stimulates intestinal epithelial renewal through a countercurrent flow

Liu, Z., Zhang, H., Lemaître, B., & Li, X. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114109 · Cited by 29

Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults

Pratt, M. B., Titlow, J., Davis, I., Barker, A. R., Dawe, H. R., Raff, J. W., & Roque, H. (2016) · Journal of Cell Science · doi:10.1242/jcs.194621 · Cited by 29

Pyridoxine/pyridoxamine 5′‐phosphate oxidase (Sgll/PNPO) is important for DNA integrity and glucose homeostasis maintenance in Drosophila

Mascolo, E., Amoroso, N., Saggio, I., Merigliano, C., & Vernı̀, F. (2019) · Journal of Cellular Physiology · doi:10.1002/jcp.28990 · Cited by 29

Low affinity binding sites in an activating CRM mediate negative autoregulation of the Drosophila Hox gene Ultrabithorax

Delker, R. K., Ranade, V., Loker, R., Voutev, R., & Mann, R. S. (2019) · PLoS Genetics · doi:10.1371/journal.pgen.1008444 · Cited by 29

Mutations in NlInR1 affect normal growth and lifespan in the brown planthopper Nilaparvata lugens

Zhao, Y. J., Huang, G., & Zhang, W. (2019) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2019.103246 · Cited by 29

Gene activation by dCas9-CBP and the SAM system differ in target preference

Sajwan, S., & Mannervik, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-54179-x · Cited by 29

ebony Affects Pigmentation Divergence and Cuticular Hydrocarbons in Drosophila americana and D. novamexicana

Lamb, A. M., Wang, Z., Simmer, P., Chung, H., & Wittkopp, P. J. (2020) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2020.00184 · Cited by 26

A unified mechanism for the control of Drosophila wing growth by the morphogens Decapentaplegic and Wingless

Zecca, M., & Struhl, G. (2021) · PLoS Biology · doi:10.1371/journal.pbio.3001111 · Cited by 24

Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila

Koreman, G. T., Xu, Y., Hu, Q., Zhang, Z., Allen, S. E., Wolfner, M. F., Wang, B., & Han, C. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014255118 · Cited by 29

C-terminal residues specific to Vasa among DEAD-box helicases are required for its functions in piRNA biogenesis and embryonic patterning

Dehghani, M., & Lasko, P. F. (2016) · Development Genes and Evolution · doi:10.1007/s00427-016-0560-5 · Cited by 27

Olfactory sensory neuron population expansions influence projection neuron adaptation and enhance odour tracking

Takagi, S., Sancer, G., Abuin, L., Stupski, S. D., Arguello, J. R., Prieto-Godino, L. L., Stern, D. L., Cruchet, S., Álvarez-Ocaña, R., Wienecke, C. F. R., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-50808-w · Cited by 29

Tissue-specific tagging of endogenous loci in Drosophila melanogaster

Koles, K., Yeh, A. R., & Rodal, A. A. (2015) · Biology Open · doi:10.1242/bio.016089 · Cited by 28

A phylogenetic profiling approach identifies novel ciliogenesis genes in Drosophila and C. elegans

Dobbelaere, J., Su, T. Y., Érdi, B., Schleiffer, A., & Dammermann, A. (2023) · The EMBO Journal · doi:10.15252/embj.2023113616 · Cited by 22

Hippo Stabilises Its Adaptor Salvador by Antagonising the HECT Ubiquitin Ligase Herc4

Aerne, B. L., Gailite, I., Sims, D., & Tapon, N. (2015) · PLoS ONE · doi:10.1371/journal.pone.0131113 · Cited by 27

Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila

Zhang, S., Wu, S. H., Yao, R., Wei, X., Ohlstein, B., & Guo, Z. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.11.016 · Cited by 26

A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila

Tang, Q., Rui, M., Bu, S., Wang, Y., Chew, L. Y., & Yu, F. (2020) · The EMBO Journal · doi:10.15252/embj.2019103549 · Cited by 28

The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction

Herédia, F., Volonté, Y., Pereirinha, J., Fernandez-Acosta, M., Casimiro, A. P., Belém, C. G., Viegas, F., Tanaka, K., Menezes, J., Arana, M., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23218-5 · Cited by 24

Damage-responsive neuro-glial clusters coordinate the recruitment of dormant neural stem cells in Drosophila

Simões, A. R., Neto, M., Alves, C. S., Santos, M. B., Fernández‐Hernández, I., Veiga‐Fernandes, H., Brea, D., Durá, I., Encinas, J. M., & Rhiner, C. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.05.015 · Cited by 29

Establishment of highly efficient transgenic system for black soldier fly (Hermetia illucens)

Kou, Z., Luo, X., Jiang, Y., Chen, B., Song, Y. F., Wang, Y., Xu, J. Q., Tomberlin, J. K., & Huang, Y. (2022) · Insect Science · doi:10.1111/1744-7917.13147 · Cited by 27

A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels

Dutta, D., Kanca, O., Byeon, S. K., Marcogliese, P. C., Zuo, Z., Shridharan, R. V., Park, J. H., Undiagnosed Diseases Network, Lin, G., Ge, M. J., et al. (2023) · Nature Metabolism · doi:10.1038/s42255-023-00873-0 · Cited by 26

UsingDrosophilato study mechanisms of hereditary hearing loss

Li, T., Bellen, H. J., & Groves, A. K. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.031492 · Cited by 27

Chemoreceptor Co-Expression in Drosophila Olfactory NeuronsPreprint

Task, D., Lin, C., Vulpe, A., Afify, A., Ballou, S., Brbić, M., Schlegel, P., Jefferis, G. S. X. E., Li, H., Menuz, K., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.11.07.355651 · Cited by 26

Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene

Stratmann, J., & Thor, S. (2017) · PLoS Genetics · doi:10.1371/journal.pgen.1006729 · Cited by 25

Ret and Substrate-Derived TGF-β Maverick Regulate Space-Filling Dendrite Growth in Drosophila Sensory Neurons

Hoyer, N., Zielke, P., Hu, C., Petersen, M., Sauter, K., Scharrenberg, R., Peng, Y. W., Kim, C. C., Han, C., Parrish, J. Z., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.07.092 · Cited by 25

DrosophilaSister-of-Sex-lethal reinforces a male-specific gene expression pattern by controllingSex-lethalalternative splicing

Moschall, R., Raß, M., Roßbach, O., Lehmann, G. J., Kullmann, L., Eichner, N., Strauß, D., Meister, G., Schneuwly, S., Krahn, M. P., et al. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky1284 · Cited by 26

The Hox transcription factor Ubx stabilizes lineage commitment by suppressing cellular plasticity in Drosophila

Domsch, K., Carnesecchi, J., Disela, V., Friedrich, J., Trost, N., Ermakova, O. V., Polychronidou, M., & Lohmann, I. (2019) · eLife · doi:10.7554/elife.42675 · Cited by 27

New Drosophila Circadian Clock Mutants Affecting Temperature Compensation Induced by Targeted Mutagenesis of Timeless

Singh, S., Giesecke, A. V., Damulewicz, M., Fexova, S. K., Mazzotta, G. M., Stanewsky, R., & Doležel, D. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.01442 · Cited by 26

The Drosophila NCAM homolog Fas2 signals independently of adhesion

Neuert, H., Deing, P., Krukkert, K., Naffin, E., Steffes, G., Risse, B., Silies, M., & Klämbt, C. (2019) · Development · doi:10.1242/dev.181479 · Cited by 26

Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS

Lacin, H., Williamson, W. R., Card, G. M., Skeath, J. B., & Truman, J. W. (2020) · eLife · doi:10.7554/elife.55007 · Cited by 26

The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission

Kim, J.-H., Ki, Y., Lee, H., Hur, M. S., Baik, B., Hur, J., Nam, D., & Lim, C. (2020) · Communications Biology · doi:10.1038/s42003-020-0902-8 · Cited by 26

DNA polymerase theta suppresses mitotic crossing over

Carvajal-Garcia, J., Crown, K. N., Ramsden, D. A., & Sekelsky, J. J. (2021) · PLoS Genetics · doi:10.1371/journal.pgen.1009267 · Cited by 26

Olfactory receptor–dependent receptor repression in Drosophila

Mika, K., Cruchet, S., Chai, P. C., Prieto-Godino, L. L., Auer, T. O., Pradervand, S., & Benton, R. (2021) · Science Advances · doi:10.1126/sciadv.abe3745 · Cited by 26

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Viswanatha, R., Mameli, E., Rodiger, J., Merckaert, P., Feitosa-Suntheimer, F., Colpitts, T. M., Mohr, S. E., Hu, Y., & Perrimon, N. (2021) · Nature Communications · doi:10.1038/s41467-021-27129-3 · Cited by 26

Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

Langmüller, A. M., Champer, J., Lapinska, S., Xie, L., Metzloff, M., Champer, S. E., Liu, J., Xu, Y., Du, J., Clark, A. G., et al. (2022) · eLife · doi:10.7554/elife.71809 · Cited by 23

Nephrotic Syndrome Gene TBC1D8B Is Required for Endosomal Maturation and Nephrin Endocytosis in Drosophila

Milosavljevic, J., Lempicki, C., Lang, K., Heinkele, H., Kampf, L. L., Leroy, C., Chen, M., Gerstner, L., Spitz, D., Wang, M., et al. (2022) · Journal of the American Society of Nephrology · doi:10.1681/asn.2022030275 · Cited by 27

Parkinsonism mutations in DNAJC6 cause lipid defects and neurodegeneration that are rescued by Synj1

Jacquemyn, J., Kuenen, S., Swerts, J., Pavie, B., Vijayan, V., Kılıç, A., Chabot, D., Wang, Y.-C., Schoovaerts, N., Corthout, N., et al. (2023) · npj Parkinson s Disease · doi:10.1038/s41531-023-00459-3 · Cited by 28

The role of Evi/Wntless in exporting Wnt proteins

Wolf, L., & Boutros, M. (2023) · Development · doi:10.1242/dev.201352 · Cited by 28

LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance

Kubrak, O. I., Jørgensen, A. F., Koyama, T., Lassen, M., Nagy, S., Hald, J., Mazzoni, G., Madsen, D., Hansen, J. B., Larsen, M. R., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-50468-w · Cited by 30

The glycosphingolipid MacCer promotes synaptic bouton formation in Drosophila by interacting with Wnt

Huang, Y., Huang, S., Scala, C. D., Wang, Q., Wandall, H. H., Fantini, J., & Zhang, Y. (2018) · eLife · doi:10.7554/elife.38183 · Cited by 25

Axonal chemokine-like Orion induces astrocyte infiltration and engulfment during mushroom body neuronal remodeling

Boulanger, A., Thinat, C., Zuchner, S. L., Fradkin, L. G., Lortat‐Jacob, H., & Dura, J. (2021) · Nature Communications · doi:10.1038/s41467-021-22054-x · Cited by 25

Co-option of immune effectors by the hormonal signalling system triggering metamorphosis in Drosophila melanogaster

Nunes, C., Koyama, T., & Sucena, É. (2021) · PLoS Genetics · doi:10.1371/journal.pgen.1009916 · Cited by 25

Regulation of Drosophila courtship behavior by the Tlx/tailless-like nuclear receptor, dissatisfaction

Duckhorn, J. C., Cande, J., Metkus, M. C., Song, H., Altamirano, S., Stern, D. L., & Shirangi, T. R. (2022) · Current Biology · doi:10.1016/j.cub.2022.02.031 · Cited by 20

Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins

Korona, D., Dirnberger, B., Giachello, C. N., Queiroz, R. M. L., Popovic, R., Müller, K. H., Minde, D., Deery, M. J., Johnson, G., Firth, L. C., et al. (2022) · eLife · doi:10.7554/elife.74322 · Cited by 25

DrosophilaTachykininergic Neurons Modulate the Activity of Two Groups of Receptor-Expressing Neurons to Regulate Aggressive Tone

Wohl, M. P., Liu, J., & Asahina, K. (2023) · Journal of Neuroscience · doi:10.1523/jneurosci.1734-22.2023 · Cited by 27

Direct control of somatic stem cell proliferation factors by the Drosophila testis stem cell niche

Albert, E. A., Puretskaia, O. A., Terekhanova, N. V., Labudina, A. A., & Bökel, C. (2018) · Development · doi:10.1242/dev.156315 · Cited by 21

O-GlcNAcase contributes to cognitive function in Drosophila

Muha, V., Fencková, M., Ferenbach, A. T., Catinozzi, M., Eidhof, I., Storkebaum, E., Schenck, A., & van Aalten, D. M. F. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.010312 · Cited by 24

Glycolysis regulates Hedgehog signalling via the plasma membrane potential

Spannl, S., Buhl, T., Nellas, I., Zeidan, S. A., Iyer, K. V., Khaliullina, H., Schultz, C., Nadler, A., Dye, N. A., & Eaton, S. (2020) · The EMBO Journal · doi:10.15252/embj.2019101767 · Cited by 23

Redundant functions of the SLC5A transporters Rumpel, Bumpel, and Kumpel in ensheathing glial cells

Yildirim, K., Winkler, B., Pogodalla, N., Mackensen, S., Baldenius, M., Garcia, L., Naffin, E., Rodrigues, F., & Klämbt, C. (2021) · Biology Open · doi:10.1242/bio.059128 · Cited by 24

Endogenous tagging of Unc-13 reveals nanoscale reorganization at active zones during presynaptic homeostatic potentiation

Dannhäuser, S., Mrestani, A., Gundelach, F., Pauli, M., Komma, F., Kollmannsberger, P., Sauer, M., Heckmann, M., & Paul, M. M. (2022) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2022.1074304 · Cited by 22

Accelerated genome engineering through multiplexing

Bao, Z., Cobb, R. E., & Zhao, H. (2015) · WIREs Systems Biology and Medicine · doi:10.1002/wsbm.1319 · Cited by 24

The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development

Farnworth, M. S., Eckermann, K. N., Ahmed, H. M. M., Mühlen, D., He, B., & Bucher, G. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9732-9_11 · Cited by 21

Drosophila Atg9 regulates the actin cytoskeleton via interactions with profilin and Ena

Kiss, V., Jipa, A., Varga, K., Takáts, S., Maruzs, T., Lőrincz, P., Simon‐Vecsei, Z., Szikora, S., Földi, I., Bajusz, C., et al. (2019) · Cell Death and Differentiation · doi:10.1038/s41418-019-0452-0 · Cited by 26

CRISPR/Cas9-Mediated Knockin and Knockout in Zebrafish

Albadri, S., Santis, F. D., Donato, V. D., & Bene, F. D. (2017) · Research and perspectives in neurosciences · doi:10.1007/978-3-319-60192-2_4 · Cited by 23

Drosophila architectural protein CTCF is not essential for fly survival and is able to function independently of CP190

Kyrchanova, O., Klimenko, N. S., Postika, N., Bonchuk, A. N., Zolotarev, N., Maksimenko, O. G., & Georgiev, P. G. (2021) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2021.194733 · Cited by 24

Cas9/Nickase-induced allelic conversion by homologous chromosome-templated repair in Drosophila somatic cells

Roy, S., Juste, S. S., Sneider, M., Auradkar, A., Klanseck, C. F., Li, Z., Julio, A. H. F., Amo, V. L. D., Bier, E., & Guichard, A. (2022) · Science Advances · doi:10.1126/sciadv.abo0721 · Cited by 23

De novo apical domain formation inside the Drosophila adult midgut epithelium

Chen, J., & Johnston, D. S. (2022) · eLife · doi:10.7554/elife.76366 · Cited by 23

Expanding the Drosophila toolkit for dual control of gene expression

Zirin, J. D., Jusiak, B., Lopes, R., Ewen‐Campen, B., Bosch, J. A., Risbeck, A., Forman, C., Villalta, C., Hu, Y., & Perrimon, N. (2023) · eLife · doi:10.7554/elife.94073 · Cited by 23

The Ets protein pointed prevents both premature differentiation and dedifferentiation of Drosophila intermediate neural progenitors

Xie, Y.-G., Li, X., Deng, X., Hou, Y., O’Hara, K., Urso, A., Peng, Y., Chen, L., & Zhu, S. (2016) · Development · doi:10.1242/dev.137281 · Cited by 23

A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity

Hauswirth, A. G., Ford, K., Wang, T., Fetter, R. D., Tong, A., & Davis, G. W. (2018) · eLife · doi:10.7554/elife.31535 · Cited by 23

Harnessing CRISPR/Cas9 technology in cardiovascular disease

Rezaei, H., Khadempar, S., Farahani, N., Hosseingholi, E. Z., Hayat, S. M. G., Sathyapalan, T., & Sahebkar, A. H. (2019) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2019.03.005 · Cited by 24

Yorkie Growth-Promoting Activity Is Limited by Atg1-Mediated Phosphorylation

Tyra, L. K., Nandi, N., Tracy, C., & Krämer, H. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.01.011 · Cited by 26

Genetically Encoded CRISPR Components Yield Efficient Gene Editing in the Invasive Pest Drosophila suzukii

Kandul, N. P., Belikoff, E. J., Liu, J., Buchman, A. B., Li, F., Ah, Y., Yang, T., Shriner, I., Scott, M. J., & Akbari, O. S. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.0032 · Cited by 23

A regulator of amino acid sensing links lipid peroxidation and lipid droplet-dependent antioxidant response

Li, J., Zhang, Y., Peng, J., Yang, W., Pan, Y., Xu, Y., Yao, J., Lin, S., Li, Y., Gu, X., et al. (2025) · Molecular Cell · doi:10.1016/j.molcel.2025.08.009 · Cited by 22

The kinesin motor Klp98A mediates apical to basal Wg transport

Witte, L., Linnemannstöns, K., Schmidt, K., Honemann‐Capito, M., Grawe, F., Wodarz, A., & Gross, J. C. (2020) · Development · doi:10.1242/dev.186833 · Cited by 22

Chromosome separation during Drosophila male meiosis I requires separase-mediated cleavage of the homolog conjunction protein UNO

Weber, J., Kabakci, Z., Chaurasia, S., Brunner, E., & Lehner, C. F. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008928 · Cited by 22

CRISPR/Cas9-engineered Drosophila knock-in models to study VCP diseases

Wall, J. M., Basu, A., Zunica, E. R. M., Dubuisson, O. S., Belmont, K. P., Broussard, J. P., Kirwan, J. P., Axelrod, C. L., & Johnson, A. E. (2021) · Disease Models & Mechanisms · doi:10.1242/dmm.048603 · Cited by 21

Biallelic variants in OGDH encoding oxoglutarate dehydrogenase lead to a neurodevelopmental disorder characterized by global developmental delay, movement disorder, and metabolic abnormalities

Whittle, E. F., Chilian, M., Karimiani, E. G., Progri, H., Buhaş, D., Köse, M., Ganetzky, R. D., Toosi, M. B., Torbati, P. N., Badv, R. S., et al. (2022) · Genetics in Medicine · doi:10.1016/j.gim.2022.11.001 · Cited by 23

A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

Nagarkar-Jaiswal, S., Manivannan, S., Zuo, Z., & Bellen, H. J. (2017) · eLife · doi:10.7554/elife.26420 · Cited by 23

Anterior CNS expansion driven by brain transcription factors

Curt, J. R., Salmani, B. Y., & Thor, S. (2019) · eLife · doi:10.7554/elife.45274 · Cited by 22

cis-regulatory architecture of a short-range EGFR organizing center in the Drosophila melanogaster leg

Newcomb, S., Voutev, R., Jory, A., Delker, R. K., Slattery, M. G., & Mann, R. S. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007568 · Cited by 17

Synthetic reconstruction of the hunchback promoter specifies the role of Bicoid, Zelda and Hunchback in the dynamics of its transcription

Fernandes, G., Tran, H., Andrieu, M., Diaw, Y., Perez-Romero, C. A., Fradin, C., Coppey, M., Walczak, A. M., & Dostatni, N. (2022) · eLife · doi:10.7554/elife.74509 · Cited by 20

Novel dominant and recessive variants in human ROBO1 cause distinct neurodevelopmental defects through different mechanisms

Huang, Y., Ma, M., Mao, X. O., Pehli̇van, D., Kanca, O., Un-Candan, F., Shu, L., Akay, G., Mitani, T., Lu, S., et al. (2022) · Human Molecular Genetics · doi:10.1093/hmg/ddac070 · Cited by 21

Upregulation of ribosome biogenesis via canonical E-boxes is required for Myc-driven proliferation

Zielke, N., Vähärautio, A., Liu, J., Kivioja, T., & Taipale, J. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.03.018 · Cited by 21

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

Gratz, S. J., Cummings, A. M., Nguyen, J. N., Hamm, D. C., Donohue, L. K., Harrison, M. M., Wildonger, J., & O’Connor-Giles, K. M. (2013) · Genetics · doi:10.1534/genetics.113.152710 · Cited by 1,000

CRISPR‐Cas9 Genome Editing in Drosophila

Gratz, S. J., Rubinstein, C. D., Harrison, M. M., Wildonger, J., & O’Connor-Giles, K. M. (2015) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb3102s111 · Cited by 214

CRISPR-/Cas9-Mediated Precise and Efficient Genome Editing in Drosophila

Nyberg, K. G., & Carthew, R. W. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2541-5_6 · Cited by 7

Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila

Koreman, G. T., Xu, Y., Hu, Q., Zhang, Z., Allen, S. E., Wolfner, M. F., Wang, B., & Han, C. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014255118 · Cited by 29

Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila

Gratz, S. J., Ukken, F. P., Rubinstein, C. D., Thiede, G. H., Donohue, L. K., Cummings, A. M., & O’Connor-Giles, K. M. (2014) · Genetics · doi:10.1534/genetics.113.160713 · Cited by 934