Cited by 1,046CRISPR and Genetic EngineeringRNA regulation and diseaseAnimal Genetics and Reproduction

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

Open access

Port, Fillip, Chen, Hui-Min, Lee, Tzumin, Bullock, Simon L.·2014·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1405500111PMC PMC4115528

Published version of a preprint

Open this paper in CoChat — free account Open PDF

Abstract

Significance: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)-mediated genome engineering promises to revolutionize genetic studies in a variety of systems. Here we describe an optimized set of tools for CRISPR/Cas experiments in the model organism Drosophila melanogaster . These tools can be used for remarkably efficient germline transmission of ( i ) loss-of-function insertion and deletion mutations in essential genes or ( ii ) precise changes in the genome sequence introduced by homology-directed repair. These tools also permit efficient biallelic targeting of genes in somatic cells, thereby demonstrating a novel application of CRISPR/Cas in rapidly revealing mutant phenotypes within the organism. Our work also paves the way for high-throughput genetic screens in Drosophila with CRISPR/Cas.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1073/pnas.1405500111

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
Brand, A. H., & Perrimon, N. (1993) · Development · doi:10.1242/dev.118.2.401 · Cited by 9,510
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Mutations affecting segment number and polarity in Drosophila
Nüsslein‐Volhard, C., & Wieschaus, E. F. (1980) · Nature · doi:10.1038/287795a0 · Cited by 4,370
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.04.025 · Cited by 3,495
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Ran, F. A., Hsu, P. D., Lin, C.-Y., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013) · Cell · doi:10.1016/j.cell.2013.08.021 · Cited by 3,299
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292
RNA-programmed genome editing in human cells
Jinek, M., East, A., Cheng, A., Lin, S., Ma, E., & Doudna, J. (2013) · eLife · doi:10.7554/elife.00471 · Cited by 2,098
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2808 · Cited by 1,993 · cited 1×
“A recent study showed that truncating the target sequence of gRNAs results in improved fidelity of CRISPR/Cas in human cells (38), suggesting a straightforward approach to minimize the risk of off-target effects in Drosophila.”
Background
An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases
Bischof, J., Maeda, R., Hediger, M., Karch, F., & Basler, K. (2007) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0611511104 · Cited by 1,959

Flagged first, then by citations

Show all 39 references

Sorted by citations

Items 201–300 of 999

The Nrf2-Keap1 pathway is activated by steroid hormone signaling to govern neuronal remodeling
Chew, L. Y., Zhang, H., He, J., & Yu, F. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109466 · Cited by 53
CRISPR-Cas Genome Editing for Insect Pest Stress Management in Crop Plants
Moon, T. T., Maliha, I. J., Khan, A. A. M., Chakraborty, M., Uddin, M. S., Amin, M. R., & Islam, T. (2022) · Stresses · doi:10.3390/stresses2040034 · Cited by 53
Functional Genomics Tools for Haemonchus contortus and Lessons From Other Helminths
Britton, C., Roberts, B., & Marks, N. D. (2016) · Advances in Parasitology/Advances in parasitology · doi:10.1016/bs.apar.2016.02.017 · Cited by 53
A simple method for imaging axonal transport in aging neurons using the adult Drosophila wing
Vagnoni, A., & Bullock, S. L. (2016) · Nature Protocols · doi:10.1038/nprot.2016.112 · Cited by 53
Self-citation
Rapid and robust optogenetic control of gene expression in Drosophila
di Pietro, F., Herszterg, S., Huang, A., Bosveld, F., Alexandre, C., Sancéré, L., Pelletier, S., Joudat, A., Kapoor, V., Vincent, J., et al. (2021) · Developmental Cell · doi:10.1016/j.devcel.2021.11.016 · Cited by 52
A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms
Boukhatmi, H., & Bray, S. J. (2018) · eLife · doi:10.7554/elife.35954 · Cited by 52
Untangling a G ordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae
Xue, W., Mermans, C., Papapostolou, K. M., Lamprousi, M., Christou, I., İnak, E., Douris, V., VONTAS, J. G., Dermauw, W., & Leeuwen, T. V. (2020) · Pest Management Science · doi:10.1002/ps.6215 · Cited by 54
State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila
Zirin, J. D., Bosch, J. A., Viswanatha, R., Mohr, S. E., & Perrimon, N. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.11.006 · Cited by 58
The postsynaptic t-SNARE Syntaxin 4 controls traffic of Neuroligin 1 and Synaptotagmin 4 to regulate retrograde signaling
Harris, K. P., Zhang, Y. V., Piccioli, Z. D., Perrimon, N., & Littleton, J. T. (2016) · eLife · doi:10.7554/elife.13881 · Cited by 51
Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time
Sidor, C., Stevens, T. J., Li, J., Jérôme Boulanger, A., & Röper, K. (2020) · Developmental Cell · doi:10.1016/j.devcel.2019.12.003 · Cited by 50
Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machineryPreprint
Munafò, M., Manelli, V., Falconio, F. A., Sawle, A., Kneuss, E., Eastwood, E. L., Seah, J. W. E., Czech, B., & Hannon, G. J. (2019) · Genes & Development · doi:10.1101/gad.325662.119 · Cited by 50
A Genome-Wide CRISPR Library for High-Throughput Genetic Screening in Drosophila Cells
Bassett, A. R., Kong, L., & Liu, J. (2015) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2015.03.011 · Cited by 48
Small-Molecule Control of Super-Mendelian Inheritance in Gene Drives
Amo, V. L. D., Leger, B. S., Cox, K. J., Gill, S., Bishop, A. L., Scanlon, G. D., Walker, J. A., Gantz, V. M., & Choudhary, A. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107841 · Cited by 49
Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management
Komal, J., Desai, H. R., Samal, I., Mastinu, A., Patel, R. D., Kumar, P. V. D., Majhi, P. K., Mahanta, D. K., & Bhoi, T. K. (2023) · Plants · doi:10.3390/plants12233961 · Cited by 49
Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect
Xu, H., & O'Brochta, D. (2015) · Proceedings of the Royal Society of London. Biological Sciences · doi:10.1098/rspb.2015.0487 · Cited by 52
Stem Cell Intrinsic Hexosamine Metabolism Regulates Intestinal Adaptation to Nutrient Content
Mattila, J., Kokki, K., Hietakangas, V., & Boutros, M. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.08.011 · Cited by 49
High-Efficiency CRISPR/Cas9 Mutagenesis of the white Gene in the Milkweed Bug Oncopeltus fasciatus
Reding, K., & Pick, L. (2020) · Genetics · doi:10.1534/genetics.120.303269 · Cited by 49
The Septate Junction Protein Tsp2A Restricts Intestinal Stem Cell Activity via Endocytic Regulation of aPKC and Hippo Signaling
Xu, C., Tang, H.-W., Hung, R., Hu, Y., Ni, X., Housden, B. E., & Perrimon, N. (2019) · Cell Reports · doi:10.1016/j.celrep.2018.12.079 · Cited by 48
Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR
Li, M., Akbari, O. S., & White, B. J. (2017) · G3 Genes Genomes Genetics · doi:10.1534/g3.117.1134 · Cited by 46
Sestrin is a key regulator of stem cell function and lifespan in response to dietary amino acids
Lu, J., Temp, U., Müller-Hartmann, A., Eßer, J., Grönke, S., & Partridge, L. (2020) · Nature Aging · doi:10.1038/s43587-020-00001-7 · Cited by 46
Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery
Andreev, V. I., Yu, C., Wang, J., Schnabl, J., Tirián, L., Gehre, M., Handler, D., Duchek, P., Novatchkova, M., Baumgartner, L., et al. (2022) · Nature Structural & Molecular Biology · doi:10.1038/s41594-022-00721-x · Cited by 48
MicroRNAs Regulate Sleep and Sleep Homeostasis in Drosophila
Goodwin, P. R., Meng, A., Moore, J. K., Hobin, M., Fulga, T. A., Vactor, D. V., & Griffith, L. C. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.05.078 · Cited by 48
CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model Insect Pyrrhocoris apterus
Kotwica‐Rolinska, J., Chodáková, L., Chvalová, D., Krištofová, L., Fenclova, I., Provazník, J., Bertolutti, M., Wu, B. C.-H., & Doležel, D. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00891 · Cited by 47
Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids
Bosch, J. A., Colbeth, R., Zirin, J. D., & Perrimon, N. (2019) · Genetics · doi:10.1534/genetics.119.302819 · Cited by 47
Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua
Keenan, S. E., Blythe, S. A., Marmion, R. A., Djabrayan, N. J.-V., Wieschaus, E. F., & Shvartsman, S. Y. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.02.004 · Cited by 47
Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci
Lueke, B., Douris, V., Hopkinson, J. E., Maiwald, F., Hertlein, G., Papapostolou, K. M., Bielza, P., Tsagkarakou, A., Leeuwen, T. V., Bass, C., et al. (2020) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2020.104583 · Cited by 47
Short and long sleeping mutants reveal links between sleep and macroautophagy
Bedont, J. L., Toda, H., Shi, M., Park, C. H., Quake, C., Stein, C., Kolesnik, A., & Sehgal, A. (2021) · eLife · doi:10.7554/elife.64140 · Cited by 46
Altering the Temporal Regulation of One Transcription Factor Drives Evolutionary Trade-Offs between Head Sensory Organs
Ramaekers, A., Claeys, A., Kapun, M., Mouchel‐Vielh, E., Potier, D., Weinberger, S., Grillenzoni, N., Dardalhon-Cuménal, D., Yan, J., Wolf, R., et al. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.07.027 · Cited by 44
Blocking promiscuous activation at cryptic promoters directs cell type–specific gene expression
Kim, J., Lu, C., Srinivasan, S., Awe, S., Brehm, A., & Fuller, M. T. (2017) · Science · doi:10.1126/science.aal3096 · Cited by 39
Performance of the Cas9 Nickase System in Drosophila melanogaster
Ren, X., Yang, Z., Mao, D., Chang, Z., Qiao, H.-H., Wang, X., Sun, J., Hu, Q., Cui, Y., Liu, L.-P., et al. (2014) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.114.013821 · Cited by 40
γ-TuRC Heterogeneity Revealed by Analysis of Mozart1
Tovey, C. A., Tubman, C. E., Hamrud, E., Zhu, Z., Dyas, A., Butterfield, A. N., Fyfe, A. J., Johnson, E., & Conduit, P. T. (2018) · Current Biology · doi:10.1016/j.cub.2018.05.044 · Cited by 45
Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm
Laurinyecz, B., Vedelek, V., Kovács, A., Szilasi, K., Lipinszki, Z., Slezák, C., Darula, Z., Juhász, G., & Sinka, R. (2019) · PLoS Genetics · doi:10.1371/journal.pgen.1007987 · Cited by 45
Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii
Ahmed, H. M. M., Hildebrand, L., & Wimmer, E. A. (2019) · BMC Biotechnology · doi:10.1186/s12896-019-0588-5 · Cited by 45
Notch Mediates Inter-tissue Communication to Promote Tumorigenesis
Boukhatmi, H., Martins, T., Pillidge, Z., Kamenova, T., & Bray, S. J. (2020) · Current Biology · doi:10.1016/j.cub.2020.02.088 · Cited by 42
CAR1deletion by CRISPR/Cas9 reduces formation of ethyl carbamate from ethanol fermentation bySaccharomyces cerevisiae
Chin, Y., Kang, W.-K., Jang, H. W., Turner, T. L., & Kim, H. J. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1831-x · Cited by 45
Harnessing gene drive
Min, J., Smidler, A. L., Najjar, D., & Esvelt, K. M. (2017) · Journal of Responsible Innovation · doi:10.1080/23299460.2017.1415586 · Cited by 44
An efficient platform for generating somatic point mutations with germline transmission in the zebrafish by CRISPR/Cas9-mediated gene editing
Zhang, Y., Zhang, Z., & Ge, W. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra117.001080 · Cited by 44
A chemical-genetics approach to study the role of atypical Protein Kinase C in Drosophila
Hannaford, M. R., Loyer, N., Tonelli, F., Zoltner, M., & Januschke, J. (2019) · Development · doi:10.1242/dev.170589 · Cited by 43
Split versions of Cleave and Rescue selfish genetic elements for measured self limiting gene drive
Oberhofer, G., Ivy, T., & Hay, B. A. (2021) · PLoS Genetics · doi:10.1371/journal.pgen.1009385 · Cited by 44
Functional characterization of Pol III U6 promoters for gene knockdown and knockout in Plutella xylostella
Huang, Y., Wang, Y., Zeng, B., Liu, Z., Xu, X., Meng, Q., Huang, Y., Yang, G., Vasseur, L., Gurr, G. M., et al. (2017) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2017.08.009 · Cited by 43
Phospholipid Homeostasis Regulates Dendrite Morphogenesis in Drosophila Sensory Neurons
Meltzer, S., Bagley, J. A., Perez, G. L., O’Brien, C. E., DeVault, L., Guo, Y., Jan, L. Y., & Jan, Y. N. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.09.089 · Cited by 40
Characterization of the Genetic Architecture Underlying Eye Size Variation Within Drosophila melanogaster and Drosophila simulans
Gaspar, P., Arif, S., Sumner‐Rooney, L. H., Kittelmann, M., Bodey, A. J., Stern, D. L., Nunes, M. D. S., & McGregor, A. P. (2020) · G3 Genes Genomes Genetics · doi:10.1534/g3.119.400877 · Cited by 40
G3’MTMD3 in the insect GABA receptor subunit, RDL, confers resistance to broflanilide and fluralaner
Zhang, Y., Huang, Q., Sheng, C., Liu, G., Zhang, K., Jia, Z., Tang, T., Mao, X., Jones, A. K., Han, Z., et al. (2023) · PLoS Genetics · doi:10.1371/journal.pgen.1010814 · Cited by 42
Genome engineering: Drosophila melanogaster and beyond
Venken, K. J. T., Sarrion‐Perdigones, A., Vandeventer, P. J., Abel, N. S., Christiansen, A. E., & Hoffman, K. L. (2015) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.214 · Cited by 43
Tools and strategies for scarless allele replacement inDrosophilausing CRISPR/Cas9
Lamb, A. M., Walker, E. A., & Wittkopp, P. J. (2016) · Fly · doi:10.1080/19336934.2016.1220463 · Cited by 42
Natural variation in stochastic photoreceptor specification and color preference in Drosophila
Anderson, C., Reiss, I., Zhou, C., Cho, A., Siddiqi, H. F., Mormann, B. H., Avelis, C. M., DeFord, P., Bergland, A. O., Roberts, E., et al. (2017) · eLife · doi:10.7554/elife.29593 · Cited by 53
Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity
Tamada, M., Shi, J., Bourdot, K. S., Supriyatno, S., Palmquist, K. H., Gutierrez-Ruiz, O. L., & Zallen, J. A. (2021) · Developmental Cell · doi:10.1016/j.devcel.2021.04.012 · Cited by 42
Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms
Jacquemyn, J., Foroozandeh, J., Vints, K., Swerts, J., Verstreken, P., Gounko, N. V., Gallego, S. F., & Goodchild, R. E. (2021) · The EMBO Journal · doi:10.15252/embj.2020106914 · Cited by 42
Proteolytic activation of Growth-blocking peptides triggers calcium responses through the GPCR Mthl10 during epithelial wound detection
O’Connor, J. T., Stevens, A. C., Shannon, E. K., Akbar, F. B., LaFever, K. S., Narayanan, N. P., Gailey, C. D., Hutson, M. S., & Page, A. W. (2021) · Developmental Cell · doi:10.1016/j.devcel.2021.06.020 · Cited by 42
Transcription factor Acj6 controls dendrite targeting via a combinatorial cell-surface code
Xie, Q., Li, J., Li, H., Udeshi, N. D., Svinkina, T., Orlin, D., Kohani, S., Guajardo, R., Mani, D. R., Xu, C., et al. (2022) · Neuron · doi:10.1016/j.neuron.2022.04.026 · Cited by 42
CrisprGE: a central hub of CRISPR/Cas-based genome editing
Kaur, K., Tandon, H., Gupta, A., & Kumar, M. (2015) · Database · doi:10.1093/database/bav055 · Cited by 41
Genome Editing: From Drosophila to Non-Model Insects and Beyond
Huang, Y., Liu, Z., & Rong, Y. S. (2016) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2016.04.007 · Cited by 42
Minibrain and Wings apart control organ growth and tissue patterning through down-regulation of Capicua
Yang, L., Paul, S., Trieu, K. G., Dent, L., Froldi, F., Forés, M., Webster, K. A., Siegfried, K. R., Kondo, S., Harvey, K. F., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1609417113 · Cited by 41
Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele
Hinaux, H., Bachem, K., Battistara, M., Rossi, M., Xin, Y., Jaenichen, R., Poul, Y. L., Arnoult, L., Kobler, J. M., Kadow, I. C. G., et al. (2018) · Developmental Biology · doi:10.1016/j.ydbio.2018.04.003 · Cited by 39
Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent roles
Jipa, A., Vedelek, V., Merényi, Z., Ürmösi, A., Takáts, S., Kovács, A. L., Horváth, G. V., Sinka, R., & Juhász, G. (2020) · Autophagy · doi:10.1080/15548627.2020.1856494 · Cited by 43
Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration
Williams, A. M., Donoughe, S., Munro, E., & Horne‐Badovinac, S. (2022) · eLife · doi:10.7554/elife.78343 · Cited by 38
CRISPR‐Cas: From the Bacterial Adaptive Immune System to a Versatile Tool for Genome Engineering
Kirchner, M., & Schneider, S. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201504741 · Cited by 39
Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system
Zhou, Y., Liu, Y., Hussmann, D., Brøgger, P., Al-Saaidi, R. A., Tan, S., Lin, L., Petersen, T. S., Zhou, G. Q., Bross, P., et al. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-2128-3 · Cited by 41
Novel functions for integrin-associated proteins revealed by analysis of myofibril attachment in Drosophila
Green, H. J., Griffiths, A. G., Ylänne, J., & Brown, N. H. (2018) · eLife · doi:10.7554/elife.35783 · Cited by 40
TBC1D8B Mutations Implicate RAB11-Dependent Vesicular Trafficking in the Pathogenesis of Nephrotic Syndrome
Kampf, L. L., Schneider, R., Gerstner, L., Thünauer, R., Chen, M., Helmstädter, M., Amar, A., Onuchic-Whitford, A. C., Loza, R., Berdelı, A., et al. (2019) · Journal of the American Society of Nephrology · doi:10.1681/asn.2019040414 · Cited by 40
De novo mutations in TOMM70, a receptor of the mitochondrial import translocase, cause neurological impairment
Dutta, D., Briere, L. C., Kanca, O., Marcogliese, P. C., Walker, M., High, F. A., Vanderver, A. L., Krier, J. B., Carmichael, N. E., Callahan, C. J., et al. (2020) · Human Molecular Genetics · doi:10.1093/hmg/ddaa081 · Cited by 39
Symmetry breaking in the female germline cyst
Nashchekin, D., Busby, L. C., Jakobs, M. A., Squires, I. J., & Johnston, D. S. (2021) · Science · doi:10.1126/science.abj3125 · Cited by 40
A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila
Jiang, X., Ren, X., Sun, J., Wang, X., Qiao, H., Xu, B., Liu, L., & Ni, J. (2015) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2015.02.007 · Cited by 39
Cell type-specific translational repression of Cyclin B during meiosis in males
Baker, C. C., Gim, B. S., & Fuller, M. T. (2015) · Development · doi:10.1242/dev.122341 · Cited by 39
Opbp is a new architectural/insulator protein required for ribosomal gene expression
Zolotarev, N., Maksimenko, O. G., Kyrchanova, O., Sokolinskaya, E. L., Osadchiy, I. S., Girardot, C., Bonchuk, A. N., Ciglar, L., Furlong, E. E. M., & Georgiev, P. G. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx840 · Cited by 40
CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii
Yan, Y., Ziemek, J., & Schetelig, M. F. (2020) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2020.104091 · Cited by 39
Application of CRISPR/Cas9 Technology to HBV
Lin, G., Zhang, K., & Li, J. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms161125950 · Cited by 41
FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster
Ravi, P., Trivedi, D., & Hasan, G. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007459 · Cited by 38
Disruption of mosGILT in Anopheles gambiae impairs ovarian development and Plasmodium infection
Yang, J., Schleicher, T. R., Dong, Y., Park, H. B., Lan, J.-F., Cresswell, P., Crawford, J. M., Dimopoulos, G., & Fikrig, E. (2019) · The Journal of Experimental Medicine · doi:10.1084/jem.20190682 · Cited by 38
Efficient expression of multiple guide RNAs for CRISPR/Cas genome editing
Hsieh‐Feng, V., & Yang, Y. (2020) · aBIOTECH · doi:10.1007/s42994-019-00014-w · Cited by 39
Perspective on the combined use of an independent transgenic sexing and a multifactorial reproductive sterility system to avoid resistance development against transgenic Sterile Insect Technique approaches
Eckermann, K. N., Dippel, S., Carrami, E. M., Ahmed, H. M. M., Curril, I. M., & Wimmer, E. A. (2014) · BMC Genomic Data · doi:10.1186/1471-2156-15-s2-s17 · Cited by 38
Unlimited Genetic Switches for Cell-Type-Specific Manipulation
García‐Marqués, J., Yang, C.-P., Espinosa-Medina, I., Mok, K., Koyama, M., & Lee, T. (2019) · Neuron · doi:10.1016/j.neuron.2019.07.005 · Cited by 34
Self-citation
The Drosophila chemokine–like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons
Ji, H., Wang, B., Shen, Y., Labib, D., Lei, J., Chen, X., Sapar, M. L., Boulanger, A., Dura, J., & Han, C. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2303392120 · Cited by 35
Daisy quorum drives for the genetic restoration of wild populationsPreprint
Min, J., Noble, C., Najjar, D., & Esvelt, K. M. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/115618 · Cited by 37
A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila
Kaya-Çopur, A., & Schnorrer, F. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6371-3_6 · Cited by 35
Defects in the Neuroendocrine Axis Contribute to Global Development Delay in a Drosophila Model of NGLY1 Deficiency
Rodriguez, T. P., Mast, J. D., Hartl, T. A., Lee, T., Sand, P., & Perlstein, E. O. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.300578 · Cited by 36
Self-citation
Snail synchronizes endocycling in a TOR-dependent manner to coordinate entry and escape from endoreplication pausing during the Drosophila critical weight checkpoint
Zeng, J., Huynh, N., Phelps, B., & King‐Jones, K. (2020) · PLoS Biology · doi:10.1371/journal.pbio.3000609 · Cited by 33
Cell-type–specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila
Kamiyama, R., Banzai, K., Liu, P., Marar, A., Tamura, R., Jiang, F., Fitch, M., Xie, J., & Kamiyama, D. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2024690118 · Cited by 36
A PROTAC targets splicing factor 3B1
Gama-Brambila, R. A., Chen, J., Zhou, J., Tascher, G., Münch, C., & Cheng, X. (2021) · Cell chemical biology · doi:10.1016/j.chembiol.2021.04.018 · Cited by 37
Cholecystokinin-like peptide mediates satiety by inhibiting sugar attraction
Guo, D., Zhang, Y., Zhang, S., Li, J., Guo, C., Pan, Y., Zhang, N., Liu, C., Jia, Y.-L., Li, C., et al. (2021) · PLoS Genetics · doi:10.1371/journal.pgen.1009724 · Cited by 36
Essential long-range action of Wingless/Wnt in adult intestinal compartmentalization
Tian, A., Duwadi, D., Benchabane, H., & Ahmed, Y. F. (2019) · PLoS Genetics · doi:10.1371/journal.pgen.1008111 · Cited by 35
Kinesin light chain-1 serine-460 phosphorylation is altered in Alzheimer’s disease and regulates axonal transport and processing of the amyloid precursor protein
Mórotz, G. M., Glennon, E. B. C., Greig, J., Lau, D. H. W., Bhembre, N., Mattedi, F., Muschalik, N., Noble, W. J., Vagnoni, A., & Miller, C. C. J. (2019) · Acta Neuropathologica Communications · doi:10.1186/s40478-019-0857-5 · Cited by 35
Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner
Guan, Z., Quiñones-Frías, M. C., Akbergenova, Y., & Littleton, J. T. (2020) · eLife · doi:10.7554/elife.55443 · Cited by 33
Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation
Dobramysl, U., Jarsch, I. K., Inoue, Y., Shimo, H., Richier, B., Gadsby, J. R., Mason, J., Szalapak, A. M., Ioannou, P. S., Correia, G. P., et al. (2021) · The Journal of Cell Biology · doi:10.1083/jcb.202003052 · Cited by 37
Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly
Wong, S., Wilmott, Z. M., Saurya, S., Alvarez‐Rodrigo, I., Zhou, F., Chau, K., Goriely, A., & Raff, J. W. (2022) · The EMBO Journal · doi:10.15252/embj.2022110891 · Cited by 35
Regulation of presynaptic Ca2+ channel abundance at active zones through a balance of delivery and turnover
Cunningham, K. L., Sauvola, C. W., Tavana, S. Z., & Littleton, J. T. (2022) · eLife · doi:10.7554/elife.78648 · Cited by 33
Creating Heritable Mutations in Drosophila with CRISPR-Cas9
Port, F., & Bullock, S. L. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6371-3_7 · Cited by 35
Self-citation
The Proton-Coupled Monocarboxylate Transporter Hermes Is Necessary for Autophagy during Cell Death
Velentzas, P. D., Zhang, L., Das, G., Chang, T.-K., Nelson, C., Kobertz, W. R., & Baehrecke, E. H. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.09.015 · Cited by 31
Drosophila CHIP protects against mitochondrial dysfunction by acting downstream of Pink1 in parallel with Parkin
Chen, J., Xue, J., Ruan, J., Zhao, J., Tang, B., & Duan, R. (2017) · The FASEB Journal · doi:10.1096/fj.201700011r · Cited by 35
SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum
Wang, L., Xu, Y., Yun, S., Yuan, Q., Satpute-Krishnan, P., & Ye, Y. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112028 · Cited by 34
Advances and perspectives in the application of CRISPR/Cas9 in insects.
Chen, L., Wang, G., Zhu, Y., Xiang, H., & Wang, W. (2016) · PubMed · doi:10.13918/j.issn.2095-8137.2016.4.220 · Cited by 34
Non-Mendelian Dominant Maternal Effects Caused by CRISPR/Cas9 Transgenic Components inDrosophila melanogaster
Lin, C.-C., & Potter, C. J. (2016) · G3 Genes Genomes Genetics · doi:10.1534/g3.116.034884 · Cited by 34
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast
Hayashi, A., & Tanaka, K. (2019) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200976 · Cited by 34
The value of existing regulatory frameworks for the environmental risk assessment of agricultural pest control using gene drives
Romeis, J., Collatz, J., Glandorf, B., & Bonsall, M. B. (2020) · Environmental Science & Policy · doi:10.1016/j.envsci.2020.02.016 · Cited by 34
miRNAs and Neural Alternative Polyadenylation Specify the Virgin Behavioral State
Garaulet, D. L., Zhang, B., Lü, W., Li, E., & Lai, E. C. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.06.004 · Cited by 34
Plk4 triggers autonomous de novo centriole biogenesis and maturation
Nabais, C., Pessoa, D., de‐Carvalho, J., van Zanten, T. S., Duarte, P., Mayor, S., Carneiro, J., Telley, I. A., & Bettencourt‐Dias, M. (2021) · The Journal of Cell Biology · doi:10.1083/jcb.202008090 · Cited by 33
Cross-species models of attention-deficit/hyperactivity disorder and autism spectrum disorder
Vecchia, E. D., Mortimer, N., Palladino, V. S., Kittel‐Schneider, S., Lesch, K., Reif, A., Schenck, A., & Norton, W. (2018) · Psychiatric Genetics · doi:10.1097/ypg.0000000000000211 · Cited by 36
miR-7 Buffers Differentiation in the Developing Drosophila Visual System
Caygill, E. E., & Brand, A. H. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.07.047 · Cited by 33
Functional validation of target-site resistance mutations against sodium channel blocker insecticides (SCBIs) via molecular modeling and genome engineering in Drosophila
Samantsidis, G.-R., O’Reilly, A. O., Douris, V., & VONTAS, J. G. (2018) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2018.12.008 · Cited by 33
The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop
Abdusselamoglu, M. D., Eroğlu, E., Burkard, T. R., & Knoblich, J. A. (2019) · eLife · doi:10.7554/elife.46566 · Cited by 31

Authors: Port, F., Chen, H.-M., Lee, T., & Bullock, S. L.
Affiliations: MRC Laboratory of Molecular Biology (GB)
Howard Hughes Medical Institute (US)
ORCID: Port, Fillip
Chen, Hui-Min
Lee, Tzumin
Bullock, Simon L.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2014-07-07
Volume: 111
Issue: 29
Pages: E2967-76
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1405500111
PMC: PMC4115528
OpenAlex: W1998356880
Cited by: 1,046
References: 39
Citations by year: 2026: 57 · 2025: 73 · 2024: 93 · 2023: 98 · 2022: 108 · 2021: 113 · 2020: 125 · 2019: 104 · 2018: 88 · 2017: 67 · 2016: 58 · 2015: 48 · 2014: 13
FWCI: 25.44
Type: journal article
Language: en
Open access: green
doi.org
Licence: cc-by-nc-nd
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion, other-oa)
Funders: Howard Hughes Medical Institute
Medical Research Council (U105178790)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords: CRISPR/Cas9, Drosophila melanogaster, germline editing, somatic genome editing, homology-directed repair, loss-of-function phenotypes, high-throughput genetic screening
MeSH terms: Alleles, Animals, Base Sequence, DNA Repair, Drosophila melanogaster, Genetic Engineering, Genetic Vectors, Germ Cells, Molecular Sequence Data, Mutation, Phenotype, RNA, Genes, Insect, Gene Targeting, Transgenes, Genes, Essential, Animals, Genetically Modified, Genome, Insect, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks