The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Biophysical insight into mechanisms of sonoporation
Open accessAbstract
Significance: Gas-filled microbubbles physically oscillate in an ultrasound field and have been shown to potentiate the delivery of therapeutic payloads. The biophysical mechanisms by which vibrating microbubbles stimulate macromolecule uptake across the cell membrane, however, remain unknown. With a coupled microscopy system capable of bright-field imaging up to 25 million frames per second, we show correlations between microsecond-scale bubble oscillations and second-to-minute–scale macromolecule diffusion, uniquely highlighting that microbubble-induced shear stress is a threshold indicator for membrane pore generation. Further insight into membrane reorganization using real-time confocal microscopy demonstrates that ultrasound-triggered microbubbles create resealing pores through both layers of cellular membrane, and gaps between confluent cells. This work presents mechanistic, biophysical insight into sonoporation as a tool for local delivery of therapeutic macromolecules.
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Self-citationUltrasound-Responsive Cavitation Nuclei for Therapy and Drug Delivery
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A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
Fajrial, A. K., He, Q. Q., Wirusanti, N. I., Slansky, J. E., & Ding, X. (2020) · Theranostics · doi:10.7150/thno.43465 · Cited by 185
Metadata
- Authors
- Helfield, B., Chen, X., Watkins, S. C., & Villanueva, F. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2016-08-22
- Volume
- 113
- Issue
- 36
- Pages
- 9983-9988
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2512809301
- Cited by
- 299
- References
- 19
- FWCI
- 11.25
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Helfield, B., Chen, X., Watkins, S. C., & Villanueva, F. S. (2016). Biophysical insight into mechanisms of sonoporation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1606915113
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