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A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing

Fajrial, Apresio K., He, Qing Qing, Wirusanti, Nurul I., Slansky, Jill E., Ding, Xiaoyun·2020·Theranostics
DOI 10.7150/thno.43465PMC PMC7196308
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Figure 1 Physical forces responsible for CRISPR transfection. The CRISPR/Cas9 system can be delivered as plasmid DNA, mRNA or RNP. The driving forces for CRISPR delivery include external field such as electrical, acoustic, laser/thermal and magnetic forces. Direct physical contact such as microinjection and passing constriction can also mediate CRISPR delivery.
Figure 1 Physical forces responsible for CRISPR transfection. The CRISPR/Cas9 system can be delivered as plasmid DNA, mRNA or RNP. The driving forces for CRISPR delivery include external field such as electrical, acoustic, laser/thermal and magnetic forces. Direct physical contact such as microinjection and passing constriction can also mediate CRISPR delivery.CC BY · PubMed Central
Figure 2 Mechanical transfection platform deforms cell membrane. A. Cell squeezing device. Reproduced with permission from Proceedings of the National Academy of Sciences USA 86 Copyright 2013. B. Workflow schematic and SEM image of sharp angle constriction made of silicon, scale bar: 30 µm. Reproduced with permission from Oxford University Press 87 Copyright 2017. C. Image of cells being deformed when passing through the constriction. Reproduced with permission from American Association for the Advancement of Science 88 CC BY-NC 4.0. D. Setup of TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF) method for CRISPR transfection. Reproduced with permission from Springer Nature 89 CC BY 4.0.
Figure 2 Mechanical transfection platform deforms cell membrane. A. Cell squeezing device. Reproduced with permission from Proceedings of the National Academy of Sciences USA 86 Copyright 2013. B. Workflow schematic and SEM image of sharp angle constriction made of silicon, scale bar: 30 µm. Reproduced with permission from Oxford University Press 87 Copyright 2017. C. Image of cells being deformed when passing through the constriction. Reproduced with permission from American Association for the Advancement of Science 88 CC BY-NC 4.0. D. Setup of TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF) method for CRISPR transfection. Reproduced with permission from Springer Nature 89 CC BY 4.0.CC BY · PubMed Central
Figure 3 Novel electroporation platform for transfection. A. Schematic of 3D microfluidic electroporation system. Reproduced from Ref. 92 with permission from The Royal Society of Chemistry. B. Design for in situ electroporation microsystem. Reproduced with permission from Springer Nature 93 CC BY 4.0. C. Cuvette design for tube electroporation. Reproduced with permission from Springer Nature 94 CC BY 4.0. D. Nanostructure electroporation system (left) and corresponding mRNA transfection efficiency showing higher uniformity (red) compared to lipofection (gray) (right). Reproduced with permission from American Association for the Advancement of Science 95 CC BY-NC 4.0.
Figure 3 Novel electroporation platform for transfection. A. Schematic of 3D microfluidic electroporation system. Reproduced from Ref. 92 with permission from The Royal Society of Chemistry. B. Design for in situ electroporation microsystem. Reproduced with permission from Springer Nature 93 CC BY 4.0. C. Cuvette design for tube electroporation. Reproduced with permission from Springer Nature 94 CC BY 4.0. D. Nanostructure electroporation system (left) and corresponding mRNA transfection efficiency showing higher uniformity (red) compared to lipofection (gray) (right). Reproduced with permission from American Association for the Advancement of Science 95 CC BY-NC 4.0.CC BY · PubMed Central
Figure 4 Acoustoporation methods for cell transfection.
Figure 4 Acoustoporation methods for cell transfection.CC BY · PubMed Central
Figure 5 Laser optoporation methods for cell transfection. A. Workflow of transfection principles using laser optoporation. Reprinted from 130, Copyright 2013, with permission from Elsevier. B. On demand transfection triggered via laser and responsive particle. Reproduced with permission from 131 Copyright 2019 Wiley.
Figure 5 Laser optoporation methods for cell transfection. A. Workflow of transfection principles using laser optoporation. Reprinted from 130, Copyright 2013, with permission from Elsevier. B. On demand transfection triggered via laser and responsive particle. Reproduced with permission from 131 Copyright 2019 Wiley.CC BY · PubMed Central

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Metadata

Authors
Fajrial, A. K., He, Q. Q., Wirusanti, N. I., Slansky, J. E., & Ding, X.
Venue
Theranostics
Publisher
Ivyspring International Publisher
Published
2020
Volume
10
Issue
12
Pages
5532-5549
ISSN
1838-7640
DOI
10.7150/thno.43465
PMC
PMC7196308
Cited by
185
References
13
Language
en

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CoChat. (2026). Citation check: A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing. https://app.cochat.ai/papers/10.7150/thno.43465
The paper
Fajrial, A. K., He, Q. Q., Wirusanti, N. I., Slansky, J. E., & Ding, X. (2020). A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing. Theranostics. https://doi.org/10.7150/thno.43465