The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors
Open accessAbstract
Significance Understanding of myelin diseases and development of new treatment options are at least partly hampered by the limited availability of human oligodendrocytes. Induced pluripotent stem cells (iPSC) may be an ideal tool to circumvent this problem; however, rapid and efficient protocols to generate oligodendrocytes from human iPSC are currently lacking. The induction of the transcription factors SOX10, OLIG2, and NKX6.2 in iPSC-derived neural progenitors accelerates oligodendroglial differentiation significantly resulting in up to 70% of O4 + oligodendrocytes within 28 d. These oligodendrocytes myelinate the CNS during development and after demyelination, and are suitable for pharmacological screens and disease modeling. The strategy presented herein will markedly facilitate the studying of human myelin diseases and the development of screening platforms for drug discovery.
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“Somatic sources, such as fibroblasts or peripheral blood cells, can be reprogrammed into iPS cells or directly converted into neural lineages, offering versatile platforms to model MS, PMD and other neuroinflammatory or demyelinating disorders48–55.”
From From insights to innovations: evaluating preclinical paradigms in demyelinating disease therapeutics (2026) · cites this paper as [50]
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“Oligodendrocytes were derived from iPSCs as previously described [17].”
From Selective suppression of oligodendrocyte-derived amyloid beta rescues neuronal dysfunction in Alzheimer’s disease (2024) · cites this paper as [17]
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Cites
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
Irizarry, R. A. (2003) · Biostatistics · doi:10.1093/biostatistics/4.2.249 · Cited by 10,603
Lu, Q. R., Yuk, D.-I., Alberta, J., Zhu, Z.-M., Pawlitzky, I., Chan, J., McMahon, A., Stiles, C., & Rowitch, D. H. (2000) · Neuron · doi:10.1016/s0896-6273(00)80897-1 · Cited by 883
Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette
Sommer, C. A., Stadtfeld, M., Murphy, G. J., Hochedlinger, K., Kotton, D. N., & Mostoslavsky, G. (2009) · Stem Cells · doi:10.1634/stemcells.2008-1075 · Cited by 676
Micropillar arrays as a high-throughput screening platform for therapeutics in multiple sclerosis
Mei, F., Fancy, S. P. J., Shen, Y. A., Niu, J., Zhao, C., Presley, B., Miao, E., Lee, S., Mayoral, S. R., Redmond, S., et al. (2014) · Nature Medicine · doi:10.1038/nm.3618 · Cited by 595
Wang, S., Bates, J., Li, X., Schanz, S., Chandler-Militello, D., Levine, C., Maherali, N., Studer, L., Hochedlinger, K., Windrem, M., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2012.12.002 · Cited by 586
A regenerative approach to the treatment of multiple sclerosis
Deshmukh, V. A., Tardif, V., Lyssiotis, C. A., Green, C. C., Kerman, B., Kim, H. J., Padmanabhan, K., Swoboda, J. G., Ahmad, I., Kondo, T., et al. (2013) · Nature · doi:10.1038/nature12647 · Cited by 531
Reinhardt, P., Glatza, M., Hemmer, K., Tsytsyura, Y., Thiel, C. S., Höing, S., Moritz, S., Parga, J. A., Wagner, L., Bruder, J. M., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0059252 · Cited by 461
Self-citationGhetti, B. F., Oblak, A. L., Boeve, B. F., Johnson, K. A., Dickerson, B. C., & Goedert, M. (2014) · Neuropathology and Applied Neurobiology · doi:10.1111/nan.12213 · Cited by 458
A culture system to study oligodendrocyte myelination processes using engineered nanofibers
Lee, S., Leach, M. K., Redmond, S., Chong, S. Y. C., Mellon, S. H., Tuck, S. J., Feng, Z. Q., Corey, J. M., & Chan, J. R. (2012) · Nature Methods · doi:10.1038/nmeth.2105 · Cited by 455
Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo
Najm, F. J., Madhavan, M., Zaremba, A., Shick, E., Karl, R. T., Factor, D. C., Miller, T. E., Nevin, Z. S., Kantor, C., Sargent, A., et al. (2015) · Nature · doi:10.1038/nature14335 · Cited by 424
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Sorted by citations
Myelin in the Central Nervous System: Structure, Function, and Pathology
Stadelmann, C., Timmler, S., Barrantes‐Freer, A., & Simons, M. (2019) · Physiological Reviews · doi:10.1152/physrev.00031.2018 · Cited by 799
Oligodendrocytes in development, myelin generation and beyond
Kuhn, S., Gritti, L., Crooks, D., & Dombrowski, Y. (2019) · Cells · doi:10.3390/cells8111424 · Cited by 625
Modeling Alzheimer’s disease with iPSC-derived brain cells
Penney, J., Ralvenius, W. T., & Tsai, L.-H. (2020) · Molecular Psychiatry · doi:10.1038/s41380-019-0468-3 · Cited by 471
In vitro Models of Neurodegenerative Diseases
Slanzi, A., Iannoto, G., Rossi, B., Zenaro, E., & Constantin, G. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00328 · Cited by 313
Fard, M. K., van der Meer, F., Sánchez, P., Cantuti-Castelvetri, L., Mandad, S., Jäkel, S., Fornasiero, E. F., Schmitt, S., Ehrlich, M., Starost, L., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aam7816 · Cited by 249
“To extend the analysis to human cells, we generated oligodendrocytes from induced pluripotent human stem cells (iPSCs) derived from healthy human adults (29).”
BackgroundSelf-citation
Metadata
- Authors
- Ehrlich, M., Mozafari, S., Glatza, M., Starost, L., Velychko, S., Hallmann, A.-L., Cui, Q.-L., Schambach, A., Kim, K.-P., Bachelin, C., Marteyn, A., Hargus, G., Johnson, R. M., Antel, J., Sterneckert, J., Zaehres, H., Schöler, H. R., Baron-Van Evercooren, A., & Kuhlmann, T.
- Affiliations
- University Hospital Münster (DE)
- Centre National de la Recherche Scientifique (FR)
- Max Planck Institute for Molecular Biomedicine (DE)
- Montreal Neurological Institute and Hospital (CA)
- Medizinische Hochschule Hannover (DE)
- McGill University (CA)
- Center for Regenerative Therapies Dresden (DE)
- University of Münster (DE)
- INSERM, U1127, F-75013 Paris, France;
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-02-28
- Volume
- 114
- Issue
- 11
- Pages
- E2243-E2252
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5358375
- OpenAlex
- W2592644893
- Cited by
- 257
- References
- 51
- Citations by year
- 2026: 21 · 2025: 27 · 2024: 26 · 2023: 25 · 2022: 39 · 2021: 28 · 2020: 35 · 2019: 31 · 2018: 19 · 2017: 5
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-11-INBS-0011)
- National Institute of Neurological Disorders and Stroke (R25NS070697)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Keywords
- oligodendrocyte differentiation, induced pluripotent stem cells, transcription factors, SOX10, Olig2, NKX6-2, myelination, myelin diseases, demyelination, neural progenitor cells, disease modeling, drug discovery screening
- MeSH terms
- Ectopic Gene Expression, Animals, Brain, Cell Differentiation, Cells, Cultured, Demyelinating Diseases, Disease Models, Animal, Myelin Basic Protein, Humans, Mutation, Myelin Sheath, Oligodendroglia, Spinal Cord, Transcription Factors, Biomarkers, Cluster Analysis, tau Proteins, Cell Death, Oxidative Stress, Cell Lineage, Gene Expression Profiling, Mice, Induced Pluripotent Stem Cells, Neural Stem Cells, Transcriptome
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