The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Altered selectivity in an Arabidopsis metal transporter
Open accessAbstract
Plants require metals for essential functions ranging from respiration to photosynthesis. These metals also contribute to the nutritional value of plants for both humans and livestock. Additionally, plants have the ability to accumulate nonessential metals such as cadmium and lead, and this ability could be harnessed to remove pollutant metals from the environment. Designing a transporter that specifically accumulates certain cations while excluding others has exciting applications in all of these areas. The Arabidopsis root membrane protein IRT1 is likely to be responsible for uptake of iron from the soil. Like other Fe(II) transporters identified to date, IRT1 transports a variety of other cations, including the essential metals zinc and manganese as well as the toxic metal cadmium. By heterologous expression in yeast, we show here that the replacement of a glutamic acid residue at position 103 in wild-type IRT1 with alanine increases the substrate specificity of the transporter by selectively eliminating its ability to transport zinc. Two other mutations, replacing the aspartic acid residues at either positions 100 or 136 with alanine, also increase IRT1 metal selectivity by eliminating transport of both iron and manganese. A number of other conserved residues in or near transmembrane domains appear to be essential for all transport function. Therefore, this study identifies at least some of the residues important for substrate selection and transport in a protein belonging to the ZIP gene family, a large transporter family found in a wide variety of organisms.
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Cites
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Self-citationMicrocytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene
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Self-citation
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Cited by
IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth
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Self-citationA long way ahead: understanding and engineering plant metal accumulation
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Phylogenetic Relationships within Cation Transporter Families of Arabidopsis
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Iron Transport and Signaling in Plants
Curie, C., & Briat, J.-F. (2003) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.54.031902.135018 · Cited by 570
Metadata
- Authors
- Rogers, E. E., Eide, D. J., & Guerinot, M. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-10-17
- Volume
- 97
- Issue
- 22
- Pages
- 12356-12360
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2158283566
- Cited by
- 470
- References
- 28
- FWCI
- 28.33
- Type
- journal article
- Language
- en
- Open access
- green
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