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Cited by 721

The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.

Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain

Open access
Laywell, Eric D., Rakic, Pasko, Kukekov, Valery G., Holland, Eric C., Steindler, Dennis A.·2000·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.250471697
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Abstract

The mammalian brain contains a population of neural stem cells (NSC) that can both self-renew and generate progeny along the three lineage pathways of the central nervous system (CNS), but the in vivo identification and localization of NSC in the postnatal CNS has proved elusive. Recently, separate studies have implicated ciliated ependymal (CE) cells, and special subependymal zone (SEZ) astrocytes as candidates for NSC in the adult brain. In the present study, we have examined the potential of these two NSC candidates to form multipotent spherical clones—neurospheres— in vitro . We conclude that CE cells are unipotent and give rise only to cells within the glia cell lineage, although they are capable of forming spherical clones when cultured in isolation. In contrast, astrocyte monolayers from the cerebral cortex, cerebellum, spinal cord, and SEZ can form neurospheres that give rise both to neurons and glia. However, the ability to form neurospheres is restricted to astrocyte monolayers derived during the first 2 postnatal wk, except for SEZ astrocytes, which retain this capacity in the mature forebrain. We conclude that environmental factors, simulated by certain in vitro conditions, transiently confer NSC-like attributes on astrocytes during a critical period in CNS development.

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Metadata

Authors
Laywell, E. D., Rakic, P., Kukekov, V. G., Holland, E. C., & Steindler, D. A.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2000-11-28
Volume
97
Issue
25
Pages
13883-13888
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.250471697
OpenAlex
W2013485257
Cited by
721
References
25
FWCI
9.96
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. https://app.cochat.ai/papers/10.1073/pnas.250471697
The paper
Laywell, E. D., Rakic, P., Kukekov, V. G., Holland, E. C., & Steindler, D. A. (2000). Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.250471697

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.