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Cited by 526

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Visualizing lipid structure and raft domains in living cells with two-photon microscopy

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Gaus, Katharina, Gratton, Enrico, Kable, Eleanor P. W., Jones, Allan S., Gelissen, Ingrid, Kritharides, Leonard, Jessup, Wendy·2003·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.2534386100
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Abstract

The lateral organization of cellular membranes is formed by the clustering of specific lipids, such as cholesterol and sphingolipids, into highly condensed domains (termed lipid rafts). Hence such domains are distinct from the remaining membrane by their lipid structure (liquid-ordered vs. -disordered domains). Here, we directly visualize membrane lipid structure of living cells by using two-photon microscopy. In macrophages, liquid-ordered domains are particularly enriched on membrane protrusions (filopodia), adhesion points and cell–cell contacts and cover 10–15% of the cell surface at 37°C. By deconvoluting the images, we demonstrate the existence of phase separation in vivo . We compare the properties of microscopically visible domains (<1 μm 2 ), with those of isolated detergent-resistant membranes and provide evidence that membrane coverage by lipid rafts and their fluidity are principally governed by cholesterol content, thereby providing strong support for the lipid raft hypothesis.

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Metadata

Authors
Gaus, K., Gratton, E., Kable, E. P. W., Jones, A. S., Gelissen, I., Kritharides, L., & Jessup, W.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2003-12-12
Volume
100
Issue
26
Pages
15554-15559
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.2534386100
OpenAlex
W2136795628
Cited by
526
References
34
FWCI
8.79
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Visualizing lipid structure and raft domains in living cells with two-photon microscopy. https://app.cochat.ai/papers/10.1073/pnas.2534386100
The paper
Gaus, K., Gratton, E., Kable, E. P. W., Jones, A. S., Gelissen, I., Kritharides, L., & Jessup, W. (2003). Visualizing lipid structure and raft domains in living cells with two-photon microscopy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2534386100

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.