Cited by 239Microtubule and mitosis dynamicsAlzheimer's disease research and treatmentsGenetic and Kidney Cyst Diseases

Tubulin requires tau for growth onto microtubule initiating sites.

Open access
Witman, G B, Cleveland, D W, Weingarten, M D, Kirschner, M W·1976·Proceedings of the National Academy of Sciences

Abstract

Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.

Integrity signals

Registry

Crossref · 10.1073/pnas.73.11.4070

Cites

27 of 27 references verified0 citation errors · 0 not checkable

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  • Inappropriate phosphorylation of tau at serine and threonine residues flanking the microtubule binding domain can lower its affinity for tubulin and inhibit its ability to promote microtubule assembly.

    “Therefore, inappropriate phosphorylation of tau at these regions can lower its affinity for tubulin and inhibit its ability to promote microtubule assembly [15].”

    From Glial contributions to neurodegeneration in tauopathies (2017) · cites this paper as [15]

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Authors
Witman, G. B., Cleveland, D. W., Weingarten, M. D., & Kirschner, M. W.
Venue
Proceedings of the National Academy of Sciences
Published
1976-11
Volume
73
Issue
11
Pages
4070-4074
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1996731829
Cited by
238
References
27
Citations by year
2026: 4 · 2025: 9 · 2024: 4 · 2023: 11 · 2022: 11 · 2021: 18 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 10 · 2014: 10 · 2013: 10 · 2012: 6
FWCI
7.58
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
tubulin, tau protein, microtubule growth, flagellar microtubules, microtubule assembly, microtubule-associated proteins
MeSH terms
Animals, Flagella, Glycoproteins, Kinetics, Microscopy, Electron, Microtubules, Molecular Weight, Proteins, Tubulin

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