Tubulin requires tau for growth onto microtubule initiating sites.
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Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.
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Inappropriate phosphorylation of tau at serine and threonine residues flanking the microtubule binding domain can lower its affinity for tubulin and inhibit its ability to promote microtubule assembly.
“Therefore, inappropriate phosphorylation of tau at these regions can lower its affinity for tubulin and inhibit its ability to promote microtubule assembly [15].”
From Glial contributions to neurodegeneration in tauopathies (2017) · cites this paper as [15]
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- Authors
- Witman, G. B., Cleveland, D. W., Weingarten, M. D., & Kirschner, M. W.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1976-11
- Volume
- 73
- Issue
- 11
- Pages
- 4070-4074
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC431332
- OpenAlex
- W1996731829
- Cited by
- 238
- References
- 27
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 4 · 2023: 11 · 2022: 11 · 2021: 18 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 10 · 2014: 10 · 2013: 10 · 2012: 6
- FWCI
- 7.58
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- tubulin, tau protein, microtubule growth, flagellar microtubules, microtubule assembly, microtubule-associated proteins
- MeSH terms
- Animals, Flagella, Glycoproteins, Kinetics, Microscopy, Electron, Microtubules, Molecular Weight, Proteins, Tubulin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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