Cited by 239Microtubule and mitosis dynamicsAlzheimer's disease research and treatmentsGenetic and Kidney Cyst Diseases

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Tubulin requires tau for growth onto microtubule initiating sites.

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Witman, G B, Cleveland, D W, Weingarten, M D, Kirschner, M W·1976·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.73.11.4070PMC PMC431332

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Abstract

Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.

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Authors: Witman, G. B., Cleveland, D. W., Weingarten, M. D., & Kirschner, M. W.
ORCID: Witman, G B
Cleveland, D W
Kirschner, M W
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1976-11
Volume: 73
Issue: 11
Pages: 4070-4074
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.73.11.4070
PMC: PMC431332
OpenAlex: W1996731829
Cited by: 238
References: 27
Citations by year: 2026: 4 · 2025: 9 · 2024: 4 · 2023: 11 · 2022: 11 · 2021: 18 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 10 · 2014: 10 · 2013: 10 · 2012: 6
FWCI: 7.58
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords: tubulin, tau protein, microtubule growth, flagellar microtubules, microtubule assembly, microtubule-associated proteins
MeSH terms: Animals, Flagella, Glycoproteins, Kinetics, Microscopy, Electron, Microtubules, Molecular Weight, Proteins, Tubulin

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Tubulin requires tau for growth onto microtubule initiating sites.

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