Transformation in Agmenellum quadruplicatum
Open accessAbstract
A DNA-mediated transformation system for the blue-green alga Agmenellum quadruplicatum, strain PR-6, is described and characterized for DNA concentration dependence, dependence on time of exposure to DNA, phenotypic expression, sensitivity to various enzymes, and competence. The stability of the transformants has been investigated, and genetic backcross and selfing experiments have been performed. This system fulfills all of the criteria established for the well-characterized transformation systems in heterotrophic bacteria and demonstrates significant similarities to at least one of these systems for all characteristics examined. The efficiency of transformation is high. This system fills a need for a well-characterized genetic system in an oxygen-evolving photoautotroph. We have used it to transform a strain with a mutational lesion in assimilatory nitrogen metabolism to a wild-type genotype.
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The rapid growth, high-light tolerance, and broad salinity tolerance of Synechococcus sp. PCC 7002 distinguish it from many freshwater cyanobacterial models.
“The rapid growth, high-light tolerance, and broad salinity tolerance of Synechococcus sp. PCC 7002 distinguish it from many freshwater cyanobacterial models.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [65]
Synechococcus sp. PCC 7002 was initially isolated from a marine environment and tolerates high salinity and elevated light intensities.
“This unicellular cyanobacterium was initially isolated from a marine environment and is distinguished by its capacity to tolerate high salinity levels and elevated light intensities [64,65].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [65]
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“This unicellular cyanobacterium was initially isolated from a marine environment and is distinguished by its capacity to tolerate high salinity levels and elevated light intensities [64,65].”
- Authors
- Stevens, S. E., & Porter, R. D.
- Affiliations
- Pennsylvania State University (US)
- ORCID
- Stevens, S. Edward
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1980-10
- Volume
- 77
- Issue
- 10
- Pages
- 6052-6056
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC350211
- OpenAlex
- W2129050237
- Cited by
- 212
- References
- 20
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 3 · 2023: 3 · 2022: 8 · 2021: 4 · 2020: 10 · 2019: 6 · 2018: 6 · 2017: 11 · 2016: 15 · 2015: 7 · 2014: 9 · 2013: 4 · 2012: 4
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- U.S. Public Health Service
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- Agmenellum quadruplicatum, cyanobacteria, DNA-mediated gene transfer, competence, transformation efficiency, self-pollination, nitrogen assimilation
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