Improved Free Fatty Acid Production in Cyanobacteria with Synechococcus sp. PCC 7002 as Host
Open accessAbstract
Microbial free fatty acids (FFAs) have been proposed as a potential feedstock for renewable energy. The ability to directly convert carbon dioxide into FFAs makes cyanobacteria ideal hosts for renewable FFA production. Previous metabolic engineering efforts using the cyanobacterial hosts Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942 have demonstrated this direct conversion of carbon dioxide into FFAs; however, FFA yields in these hosts are limited by the negative impact of FFA production on the host cell physiology. This work investigates the use of Synechococcus sp. PCC 7002 as a cyanobacterial host for FFA production. In comparison to S. elongatus PCC 7942, Synechococcus sp. PCC 7002 strains produced and excreted FFAs at similar concentrations but without the detrimental effects on host physiology. The enhanced tolerance to FFA production with Synechococcus sp. PCC 7002 was found to be temperature-dependent, with physiological effects such as reduced photosynthetic yield and decreased photosynthetic pigments observed at higher temperatures. Additional genetic manipulations were targeted for increased FFA production, including thioesterases and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Overexpression of non-native RuBisCO subunits (rbcLS) from a psbAI promoter resulted in more than a threefold increase in FFA production, with excreted FFA concentrations reaching >130 mg/L. This work illustrates the importance of host strain selection for cyanobacterial biofuel production and demonstrates that the FFA tolerance of Synechococcus sp. PCC 7002 can allow for high yields of excreted FFA.
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Similar findings of thioesterase overexpression stimulating free FA secretion have been observed in Synechococcus elongatus PCC7942 and Synechococcus sp. PCC7002.
“Similar findings have also been observed in Synechococcus elongatus PCC7942 and Synechococcus sp. PCC7002 [191, 192].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [192]
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- Authors
- Ruffing, A. M.
- Affiliations
- Sandia National Laboratories (US)
- Corresponding author
- Ruffing, Anne M.
- ORCID
- Ruffing, Anne M.
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2014-05-26
- Volume
- 2
- Pages
- 17-17
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC4126656
- OpenAlex
- W2101423655
- Cited by
- 181
- References
- 36
- Citations by year
- 2026: 5 · 2025: 17 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 20 · 2020: 16 · 2019: 13 · 2018: 23 · 2017: 16 · 2016: 17 · 2015: 12 · 2014: 2
- FWCI
- 6.43
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- U.S. Department of Energy (DE-ACO4-94AL85000)
- Laboratory Directed Research and Development
- Sandia National Laboratories (94AL85000)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- free fatty acid production, Synechococcus sp. PCC 7002, cyanobacteria, CO2 fixation, thioesterase, photosynthetic yield, photosynthetic pigments, temperature effects, host strain selection, biofuel feedstock, metabolic engineering
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