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Cited by 497

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.

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Panicali, D, Paoletti, E·1982·Proceedings of the National Academy of Sciences

Abstract

We have constructed recombinant vaccinia viruses containing the thymidine kinase gene from herpes simplex virus. The gene was inserted into the genome of a variant of vaccinia virus that had undergone spontaneous deletion as well as into the 120-megadalton genome of the large prototypic vaccinia variant. This was accomplished via in vivo recombination by cotransfection of eukaryotic tissue culture cells with cloned BamHI-digested thymidine kinase gene from herpes simplex virus containing flanking vaccinia virus DNA sequences and infectious rescuing vaccinia virus. Pure populations of the recombinant viruses were obtained by replica filter techniques or by growth of the recombinant virus in biochemically selective medium. The herpes simplex virus thymidine kinase gene, as an insert in vaccinia virus, is transcribed in vivo and in vitro, and the fidelity of in vivo transcription into a functional gene product was detected by the phosphorylation of 5-[125I]iodo-2'-deoxycytidine.

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Metadata

Authors
Panicali, D., & Paoletti, E.
Venue
Proceedings of the National Academy of Sciences
Published
1982-08
Volume
79
Issue
16
Pages
4927-4931
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1996299849
Cited by
497
References
2
FWCI
12.76
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. https://app.cochat.ai/papers/10.1073/pnas.79.16.4927
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Panicali, D., & Paoletti, E. (1982). Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.16.4927

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.