The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.
Open accessAbstract
We have constructed recombinant vaccinia viruses containing the thymidine kinase gene from herpes simplex virus. The gene was inserted into the genome of a variant of vaccinia virus that had undergone spontaneous deletion as well as into the 120-megadalton genome of the large prototypic vaccinia variant. This was accomplished via in vivo recombination by cotransfection of eukaryotic tissue culture cells with cloned BamHI-digested thymidine kinase gene from herpes simplex virus containing flanking vaccinia virus DNA sequences and infectious rescuing vaccinia virus. Pure populations of the recombinant viruses were obtained by replica filter techniques or by growth of the recombinant virus in biochemically selective medium. The herpes simplex virus thymidine kinase gene, as an insert in vaccinia virus, is transcribed in vivo and in vitro, and the fidelity of in vivo transcription into a functional gene product was detected by the phosphorylation of 5-[125I]iodo-2'-deoxycytidine.
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Cites
Translation of rabbit globin mRNA introduced by liposomes into mouse lymphocytes
DIMITRIADIS, G. J. (1978) · Nature · doi:10.1038/274923a0 · Cited by 212
OSTRO, M. J., GIACOMONI, D., LAVELLE, D., PAXTON, W., & DRAY, S. (1978) · Nature · doi:10.1038/274921a0 · Cited by 154
Cited by
Fuerst, T. R., Niles, E. G., Studier, F. W., & Moss, B. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.21.8122 · Cited by 2,089
The complete DNA sequence of vaccinia virus
Goebel, S. J., Johnson, G. P., Perkus, M. E., Davis, S. W., Winslow, J. P., & Paoletti, E. (1990) · Virology · doi:10.1016/0042-6822(90)90294-2 · Cited by 850
Self-citationExpression of rabies virus glycoprotein from a recombinant vaccinia virus
Kieny, M. P., Lathe, R., Drillien, R., Spehner, D., Skory, S., Schmitt, D., Wiktor, T., Koprowski, H., & Lecocq, J. P. (1984) · Nature · doi:10.1038/312163a0 · Cited by 444
Tratschin, J. D., West, M. H., Sandbank, T., & Carter, B. J. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.10.2072 · Cited by 202
Identification and Cloning of the Fowlpox Virus Thymidine Kinase Gene Using Vaccinia Virus
Boyle, D. B., & Coupar, B. E. H. (1986) · Journal of General Virology · doi:10.1099/0022-1317-67-8-1591 · Cited by 51
Metadata
- Authors
- Panicali, D., & Paoletti, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1982-08
- Volume
- 79
- Issue
- 16
- Pages
- 4927-4931
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1996299849
- Cited by
- 497
- References
- 2
- FWCI
- 12.76
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. https://app.cochat.ai/papers/10.1073/pnas.79.16.4927
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- Panicali, D., & Paoletti, E. (1982). Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.16.4927
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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