The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.
Open accessAbstract
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,249
Organization and Expression of Eucaryotic Split Genes Coding for Proteins
Breathnach, R., & Chambon, P. (1981) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.50.070181.002025 · Cited by 4,548
A membrane-filter technique for the detection of complementary DNA
Denhardt, D. T. (1966) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(66)90447-5 · Cited by 3,885
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,265
Wahl, G. M., Stern, M., & Stark, G. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3683 · Cited by 3,247
A system for shotgun DNA sequencing
Messing, J., Crea, R., & Seeburg, P. H. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.2.309 · Cited by 2,619
Nucleotide sequence of the rightward operator of phage lambda.
Maniatis, T., Jeffrey, A., & Kleid, D. G. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.1184 · Cited by 2,030
Clewell, D. B., & Helinski, D. R. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.62.4.1159 · Cited by 1,820
Radloff, R., Bauer, W., & Vinograd, J. (1967) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.57.5.1514 · Cited by 1,319
Cited by
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
Kozak, M. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.2.857 · Cited by 3,293
Phenotypic and functional separation of memory and effector human CD8+ T cells
Hamann, D., Baars, P. A., Rep, M. H. G., Hooibrink, B., Kerkhof-Garde, S. R., Klein, M. R., & Lier, R. A. W. V. (1997) · The Journal of Experimental Medicine · doi:10.1084/jem.186.9.1407 · Cited by 1,366
Miller, A. D., & Buttimore, C. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.8.2895 · Cited by 1,370
Mutator phenotype of Werner syndrome is characterized by extensive deletions.
Fukuchi, K., Martin, G. M., & Monnat, R. J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.15.5893 · Cited by 419
Translational Suppression by Trinucleotide Repeat Expansion at FMR1
Feng, Y., Zhang, F., Lokey, L. K., Chastain, J. L., Lakkis, L., Eberhart, D., & Warren, S. T. (1995) · Science · doi:10.1126/science.7732383 · Cited by 358
Metadata
- Authors
- Jolly, D. J., Okayama, H., Berg, P., Esty, A. C., Filpula, D., Bohlen, P., Johnson, G. G., Shively, J. E., Hunkapillar, T., & Friedmann, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-01
- Volume
- 80
- Issue
- 2
- Pages
- 477-481
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2060570369
- Cited by
- 324
- References
- 22
- FWCI
- 10.27
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase. https://app.cochat.ai/papers/10.1073/pnas.80.2.477
- The paper
- Jolly, D. J., Okayama, H., Berg, P., Esty, A. C., Filpula, D., Bohlen, P., Johnson, G. G., Shively, J. E., Hunkapillar, T., & Friedmann, T. (1983). Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.80.2.477
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
