The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2).
Open accessAbstract
Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column and then on a Mono P column with fast protein liquid chromatography and reversed-phase HPLC. A 5300-fold enrichment in specific activity of BCDF with about 25% recovery was attained. The homogeneity of purified BCDF was evidenced by the following: (i) the specific activity was 1.7 X 10(7) units/mg of protein, (ii) only two bands, Mr 19,000 and 21,000, were identified by NaDodSO4/PAGE under reduced as well as nonreduced conditions, and (iii) BCDF activity was recovered from the gel after NaDodSO4/PAGE in the fractions corresponding to protein bands of Mr 19,000 or 21,000. Purified BCDF induced Ig secretion in Epstein-Barr virus-transformed cell lines; as little as 3 pM gave 50% of the maximum reaction achieved by 30-80 pM BCDF. Purified BCDF induced Ig production in activated B cells without any effect on cell growth. Purified BCDF did not show any activity of interleukin 1 or 2, B-cell stimulatory factor (BSF)p-1, B-cell growth factor II (BCGF-II), or interferon. Since BCDF was isolated and characterized as described, we propose that the BCDF that induces the final differentiation of B cells into high-rate Ig-secreting cells be designated BSFp-2.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Identification of a T cell-derived b cell growth factor distinct from interleukin 2.
Howard, M., Farrar, J., Hilfiker, M., Johnson, B., Takatsu, K., Hamaoka, T., & Paul, W. E. (1982) · The Journal of experimental medicine · doi:10.1084/jem.155.3.914 · Cited by 1,099
Replacement of T-Cell Function by a T-Cell Product
SCHIMPL, A., & WECKER, E. (1972) · Nature New Biology · doi:10.1038/newbio237015a0 · Cited by 529
T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
Isakson, P. C., Puré, E., Vitetta, E. S., & Krammer, P. H. (1982) · The Journal of experimental medicine · doi:10.1084/jem.155.3.734 · Cited by 442
Regulation of B-Cell Growth and Differentiation by Soluble Factors
Howard, M., & Paul, W. E. (1983) · Annual Review of Immunology · doi:10.1146/annurev.iy.01.040183.001515 · Cited by 430
Muraguchi, A., Kishimoto, T., Miki, Y., Kuritani, T., Kaieda, T., Yoshizaki, K., & Yamamura, Y. (1981) · The Journal of Immunology · doi:10.4049/jimmunol.127.2.412 · Cited by 237
Self-citationEffect of recombinant IL 2 and gamma-IFN on proliferation and differentiation of human B cells.
Nakagawa, T., Hirano, T., Nakagawa, N., Yoshizaki, K., & Kishimoto, T. (1985) · The Journal of Immunology · doi:10.4049/jimmunol.134.2.959 · Cited by 215
Self-citationSwain, S. L., Howard, M., Kappler, J., Marrack, P., Watson, J., Booth, R., Wetzel, G. D., & Dutton, R. W. (1983) · The Journal of experimental medicine · doi:10.1084/jem.158.3.822 · Cited by 200
Yoshizaki, K., Nakagawa, T., Kaieda, T., Muraguchi, A., Yamamura, Y., & Kishimoto, T. (1982) · The Journal of Immunology · doi:10.4049/jimmunol.128.3.1296 · Cited by 199
Self-citation
Cited by
The pro- and anti-inflammatory properties of the cytokine interleukin-6
Scheller, J., Chalaris, A., Schmidt-Arras, D., & Rose-John, S. (2011) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2011.01.034 · Cited by 3,212
Interleukin-6 and the acute phase response
Heinrich, P. C., Castell, J. V., & Andus, T. (1990) · Biochemical Journal · doi:10.1042/bj2650621 · Cited by 2,734
Hirano, T., Yasukawa, K., Harada, H., Taga, T., Watanabe, Y., Matsuda, T., Kashiwamura, S.-I., Nakajima, K., Koyama, K., Iwamatsu, A., et al. (1986) · Nature · doi:10.1038/324073a0 · Cited by 2,139
Self-citationAutocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas
Kawano, M., Hirano, T., Matsuda, T., Taga, T., Horii, Y., Iwato, K., Asaoku, H., Tang, B., Tanabe, O., Tanaka, H., et al. (1988) · Nature · doi:10.1038/332083a0 · Cited by 1,633
Self-citationInterleukin-6 triggers the association of its receptor with a possible signal transducer, gp130
Taga, T., Hibi, M., Hirata, Y., Yamasaki, K., Yasukawa, K., Matsuda, T., Hirano, T., & Kishimoto, T. (1989) · Cell · doi:10.1016/0092-8674(89)90438-8 · Cited by 1,396
Self-citation
Metadata
- Authors
- Hirano, T., Taga, T., Nakano, N., Yasukawa, K., Kashiwamura, S., Shimizu, K., Nakajima, K., Pyun, K. H., & Kishimoto, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-08
- Volume
- 82
- Issue
- 16
- Pages
- 5490-5494
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1971615288
- Cited by
- 677
- References
- 24
- FWCI
- 9.79
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). https://app.cochat.ai/papers/10.1073/pnas.82.16.5490
- The paper
- Hirano, T., Taga, T., Nakano, N., Yasukawa, K., Kashiwamura, S., Shimizu, K., Nakajima, K., Pyun, K. H., & Kishimoto, T. (1985). Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.16.5490
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
