The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography.
Open accessAbstract
Implantation of demineralized diaphyseal bone matrix in subcutaneous sites induces a sequence of events resulting in the local differentiation of endochondral bone. Demineralized bovine bone matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the bone-inductive proteins were purified greater than 12,000-fold. The purification steps include affinity chromatography on heparin-Sepharose, hydroxyapatite chromatography, gel filtration, and C18 reverse-phase HPLC. Since the purified protein in conjunction with insoluble collagenous bone matrix induced new bone differentiation in vivo we have designated this component osteogenin. The osteogenic potential is specific for osteogenin and is not exhibited by previously isolated growth factors.
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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Bone: Formation by Autoinduction
Urist, M. R. (1965) · Science · doi:10.1126/science.150.3698.893 · Cited by 5,546
Carpenter, G., & Cohen, S. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.001205 · Cited by 2,909
Transforming Growth Factor-β: Biological Function and Chemical Structure
Sporn, M. B., Roberts, A. B., Wakefield, L. M., & Assoian, R. K. (1986) · Science · doi:10.1126/science.3487831 · Cited by 1,182
(1990) · Elsevier eBooks · doi:10.1016/c2009-0-34738-8 · Cited by 1,069
Extracellular matrix biochemistry
Piez, K. A., & Reddi, A. H. (1984) · Elsevier eBooks · Cited by 1,034
Self-citationHauschka, P. V., Mavrakos, A. E., Iafrati, M. D., Doleman, S. E., & Klagsbrun, M. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67143-1 · Cited by 869
Biochemical Sequences in the Transformation of Normal Fibroblasts in Adolescent Rats
Reddi, A. H., & Huggins, C. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.6.1601 · Cited by 810
Self-citationSampath, T. K., & Reddi, A. H. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.12.7599 · Cited by 588
Self-citationCartilage-inducing factor-A. Apparent identity to transforming growth factor-beta.
Seyedin, S. M., Thompson, A. Y., Bentz, H., Rosen, D. M., McPherson, J. M., Conti, A., Siegel, N. R., Galluppi, G. R., & Piez, K. A. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38436-3 · Cited by 521
Cited by
Recombinant human bone morphogenetic protein induces bone formation.
Wang, E. A., Rosen, V, D'Alessandro, J. S., Bauduy, M., Cordes, P., Harada, T., Israel, D. I., Hewick, R. M., Kerns, K. M., & LaPan, P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.6.2220 · Cited by 1,523
BMP signalling in skeletal development, disease and repair
Salazar, V. S., Gamer, L. W., & Rosen, V. (2016) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.12 · Cited by 822
Yamaguchi, A., Komori, T., & Suda, T. (2000) · Endocrine Reviews · doi:10.1210/edrv.21.4.0403 · Cited by 696
Purification and characterization of other distinct bone-inducing factors.
Wang, E. A., Rosen, V, Cordes, P., Hewick, R. M., Kriz, M. J., Luxenberg, D. P., Sibley, B. S., & Wozney, J. M. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.24.9484 · Cited by 614
In Vivo Stimulation of Bone Formation by Transforming Growth Factor-β
NODA, M., & CAMILLIERE, J. J. (1989) · Endocrinology · doi:10.1210/endo-124-6-2991 · Cited by 578
Metadata
- Authors
- Sampath, T. K., Muthukumaran, N., & Reddi, A. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-10
- Volume
- 84
- Issue
- 20
- Pages
- 7109-7113
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2163788818
- Cited by
- 332
- References
- 19
- FWCI
- 20.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Sampath, T. K., Muthukumaran, N., & Reddi, A. H. (1987). Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.20.7109
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
